明胶 (Gelatin)是一种天然大分子材料，其分子骨架上含有细胞黏附的RGD序列以及酶可降解位点，有利于细胞的粘附、增殖与分化。
GelMA是由明胶 (Gelatin)经甲基丙烯酸酐 (MA)修饰得到的、具有光敏特性的高分子材料，可与蓝光或紫外光引发剂配合使用，在蓝光或紫外光辐照下交联固化。该高分子具有优异的生物相容性，其高分子骨架上含有细胞黏附的RGD序列以及酶可降解位点，有利于细胞的粘附、增殖与分化，且固化简单，现已被广泛应用在细胞2D/3D培养、组织工程、再生医学、类器官芯片、生物3D打印、生物传感器等领域。
产品规格
| 组分 | 外观 | 规格 | 备注 |
| GelMA | 微黄色至黄色粉末或颗粒 | 1 g/瓶、5 g/瓶 | 避光保存 |
使用建议
1、GelMA可与邻硝基苄醇化高分子，如HANB、GelNB联合使用。二者由光引发剂LAP、NAP光照引发聚合并交联，仅需数秒即可获得高强度水凝胶材料。
2、GelMA可与甲基丙烯基修饰的高分子，如CMCMA、HAMA、CSMA、ChMA联合使用，由光引发剂光照引发聚合，构建不同理化性能的水凝胶材料。
3、GelMA单独使用，由光引发剂光照引发聚合并交联。
配制方法
1、配制光引发剂标准液：取一定质量的NAP或LAP配制成浓度为0.1 wt%-0.25 wt%范围内的溶液，2~8 ℃避光保存；
2、取所需质量的GelMA放入离心管，并取所需体积的引发剂标准溶液加入到上述离心管中；
3、将上述样品于37 ℃摇床内避光振荡，直至完全溶解；或于热水浴避光加热至完全溶解，期间涡旋或振荡数次。
注意事项
1、GelMA的标记率越高，配制的浓度越高，固化后形成的凝胶模量越大，固化时间越短。
2、GelMA的建议使用浓度不超过30 wt%。
3、光引发剂标准液浓度越高，GelMA溶液固化速度越快，固化后形成的凝胶越脆。
产品应用
细胞2D/3D培养、组织工程、再生医学、类器官芯片、生物3D打印、生物传感器等领域。
灭菌方式
1、过滤灭菌 (建议)：使用0.22 μm无菌针头过滤器过滤溶液灭菌，需趁热过滤，此方法仅限于低粘度溶液使用，如溶液粘度较高时，请选用巴氏灭菌或湿热灭菌法；
2、巴氏灭菌：将溶液加热到80 ℃，保持30 min；再迅速转移至冰水混合物中冷却至常温。共循环上述操作三次；
3、热灭菌：将溶液于高温高压灭菌锅内，121 ℃下灭菌8 min，手动放气后，将溶液迅速转移至冰水混合物中冷却至常温。需注意，高温条件会导致凝胶性能下降，因此使用该方法灭菌时应严格遵守上述操作步骤。
注：无菌溶液可以2-8 ℃避光暂存，建议7天内使用；非无菌溶液可以2-8 ℃避光暂存，建议48小时内使用。
产品特点
1、提供细胞粘附位点，实现细胞的定向生长和分化
2、具备光交联特性，可用于3D打印复杂的组织结构
典型应用
1、血管再生：细胞在微图案结构内排列和组织，并组装形成有组织的肌动蛋白纤维和圆形/椭圆形横截面的脐带结构。
2、骨修复：结合微流控技术快速制备高强度载细胞微凝胶实现骨缺损的修复
3、3D打印：结合纳米粘土打印具有高形状保真度的复杂支架。
产品优势
1、批次稳定：采用标准化的生产工艺，保证不同批次的GelMA在成分、理化特性和生物学方面具有高度的重复性和可控性。
2、超高纯度：采用独特的生产工艺，有效地去除甲基丙烯酸小分子和工艺杂质，实现超低杂质水平，保证GelMA在生物医学应用的安全性。
3、可靠的安全性GelMA已经第三方机构完成生物相容性评价，产品安全无毒性，加速下游产品从实验室到临床的转化。每批产品均经过严格的标准化的检验后放行，保证 GelMA在生物医学应用的安全性。
4、高稳定性：参照《无源植入性医疗器械稳定性研究指导原则》，经过实时稳定性和加速稳定性试验证明：GelMA产品在标示贮存条件下质量稳定。
Product Introduction
Gelatin is a natural macromolecular material whose molecular backbone contains cell-adhesive RGD sequences and enzyme-degradable sites, which are beneficial for cell adhesion, proliferation, and differentiation.
GelMA is a photosensitive polymer obtained by modifying gelatin with methacrylic anhydride (MA). It can be used in combination with blue light or UV photoinitiators to crosslink and solidify under blue light or UV irradiation. This polymer exhibits excellent biocompatibility; its backbone contains cell-adhesive RGD sequences and enzyme-degradable sites, which facilitate cell adhesion, proliferation, and differentiation. Additionally, its simple curing process has led to its wide application in fields such as 2D/3D cell culture, tissue engineering, regenerative medicine, organoid chips, 3D bioprinting, and biosensors.
Product Specifications
| Component | Appearance | Specification | Remarks |
| GelMA | Pale yellow to yellow powder or granules | 1 g/bottle, 5 g/bottle | Store in the dark |
Usage Recommendations
1. GelMA can be used in combination with o-nitrobenzyl alcohol-modified polymers, such as HANB and GelNB. Both can be polymerized and crosslinked by photoinitiators LAP or NAP under light irradiation, yielding high-strength hydrogel materials in just a few seconds.
2. GelMA can be used in combination with methacryloyl-modified polymers, such as CMCMA, HAMA, CSMA, and ChMA, and polymerized under light irradiation with photoinitiators to construct hydrogel materials with different physicochemical properties.
3. GelMA can be used alone, polymerized and crosslinked under light irradiation with photoinitiators.
Preparation Method
1. Prepare a photoinitiator stock solution: Dissolve a specific mass of NAP or LAP to prepare a solution with a concentration in the range of 0.1 wt%–0.25 wt%, and store at 2–8 ℃ away from light.
2. Transfer the required mass of GelMA into a centrifuge tube, and add the required volume of the initiator stock solution to the tube.
3. Incubate the sample in a 37 ℃ shaker in the dark with shaking until completely dissolved; alternatively, heat it in a hot water bath in the dark until completely dissolved, with vortexing or shaking several times during the process.
Precautions
1. The higher the degree of methacryloylation of GelMA and the higher the concentration of the prepared solution, the greater the modulus of the cured gel and the shorter the curing time.
2. The recommended working concentration of GelMA should not exceed 30 wt%.
3. The higher the concentration of the photoinitiator stock solution, the faster the curing speed of the GelMA solution and the more brittle the resulting cured gel.
Product Applications
2D/3D cell culture, tissue engineering, regenerative medicine, organoid chips, 3D bioprinting, biosensors, and other fields.
Sterilization Methods
1. Filtration sterilization (recommended): Sterilize the solution by filtration using a 0.22 μm sterile syringe filter while the solution is still warm. This method is only applicable to low-viscosity solutions. For solutions with high viscosity, use pasteurization or autoclaving.
2. Pasteurization: Heat the solution to 80 ℃ and hold for 30 min; then quickly transfer it to an ice-water mixture to cool to room temperature. Repeat this cycle three times.
3. Autoclaving: Sterilize the solution in an autoclave at 121 ℃ for 8 min. After manual pressure release, quickly transfer the solution to an ice-water mixture to cool to room temperature. Note that high temperatures can reduce gel performance, so strict adherence to the above steps is required when using this method.
Note: Sterile solutions can be stored temporarily at 2–8 ℃ away from light and are recommended for use within 7 days. Non-sterile solutions can be stored temporarily at 2–8 ℃ away from light and are recommended for use within 48 hours.
Product Features
1. Provides cell adhesion sites to enable directed cell growth and differentiation.
2. Exhibits photocrosslinking properties, suitable for 3D printing of complex tissue structures.
Typical Applications
1. Vascular regeneration: Cells align and organize within micro-patterned structures and assemble to form organized actin fibers and umbilical cord-like structures with circular/oval cross-sections.
2. Bone repair: Combined with microfluidic technology to rapidly prepare high-strength cell-laden microgels for bone defect repair.
3. 3D printing: Combined with nanoclay to print complex scaffolds with high shape fidelity.
Product Advantages
1. Batch-to-batch stability: Standardized production processes ensure high repeatability and controllability of GelMA in composition, physicochemical properties, and biological performance across different batches.
2. Ultra-high purity: Unique production processes effectively remove methacrylic acid small molecules and process impurities, achieving ultra-low impurity levels and ensuring the safety of GelMA in biomedical applications.
3. Reliable safety: GelMA has undergone biocompatibility evaluation by third-party institutions, confirming its safety and non-toxicity, accelerating the translation of downstream products from the laboratory to the clinic. Each batch is released only after strict, standardized testing to ensure safety in biomedical applications.
4. High stability: In accordance with the Guiding Principles for Stability Studies of Non-Invasive Implantable Medical Devices, real-time and accelerated stability tests have demonstrated that GelMA maintains stable quality under the indicated storage conditions.
ZJ26F0939609
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