AlgMA是由海藻酸钠 (Alg)经甲基丙烯酸酐 (MA)修饰得到的、具有光敏特性的高分子材料，可与蓝光或紫外光引发剂配合使用，在蓝光或紫外光辐照下交联固化。该高分子生物相容性优异、固化简单，具有独特的与多价阳离子螯合的能力以及较高的粘度，现已被广泛应用在细胞2D/3D培养、生物打印、药物载体、组织工程支架、细胞微囊化免疫隔离等领域。
产品规格
| 组分 | 外观 | 规格 | 备注 | AlgMA
| 白色至淡黄色粉末颗粒
| 1 g/瓶、5 g/瓶 | 避光保存 |
|
使用建议
1、AlgMA可与邻硝基苄醇化高分子，如PEGNB、HANB、GelNB联合使用。二者由光引发剂LAP、NAP光照引发聚合并交联，仅需数秒即可获得高强度水凝胶材料。
2、AlgMA可与甲基丙烯基修饰的高分子，如CMCMA、GelMA、CSMA、ChMA以及丙烯基修饰的高分子，如F127DA联合使用，由光引发剂光照引发聚合，构建不同理化性能的水凝胶材料。
3、AlgMA单独使用，由光引发剂光照引发聚合并交联。
配制方法
1、配制光引发剂标准液：取一定质量的NAP或LAP配制成浓度为0.1 wt% - 0.25 wt%范围内的溶液，2~8 ℃避光保存；
2、取所需质量的AlgMA放入离心管，并取所需体积的引发剂标准溶液加入到上述离心管中；
3、将上述样品于室温下避光搅拌或于摇床振荡，直至完全溶解。样品如在溶液中结块，可先用药匙碾碎；若样品溶解速度偏慢，可置于50-60 ℃环境加热搅拌助溶。
注意事项
1、AlgMA配制的浓度越高，固化后形成的凝胶模量越大，固化时间越短。
2、AlgMA配制的溶液粘度与原料海藻酸钠的粘度及配置的溶液浓度有关。低粘度的AlgMA建议使用浓度不超过5 wt%；中粘度的AlgMA建议使用浓度不超过2 wt%。
3、光引发剂标准液浓度越高，AlgMA溶液固化速度越快。
产品应用
3D 生物打印、肿瘤模型构建、细胞负载、创面敷料、药物控释以及齿科印模等。
灭菌方式
1、过滤灭菌 (建议)：使用0.22 μm无菌针头过滤器过滤溶液灭菌。此方法仅限于低粘度溶液使用，如溶液粘度较高时，请选用巴氏灭菌或湿热灭菌法；
2、巴氏灭菌：将溶液加热到80 ℃，保持30 min；再迅速转移至冰水混合物中冷却至常温。共循环上述操作三次；
3、湿热灭菌：将溶液于高温高压灭菌锅内，121 ℃下灭菌8 min，手动放气后，将溶液迅速转移至冰水混合物中冷却至常温。需注意，高温条件会导致凝胶性能下降，因此使用该方法灭菌时应严格遵守上述操作步骤。
备注：无菌溶液可以2-8 ℃避光暂存，建议7天内使用；非无菌溶液可以2-8 ℃避光暂存，建议48小时内使用。
产品特点
海藻酸钠的G单元中的Na+可在水溶液中与多价金属阳离子 (Zn²⁺、Fe³⁺、Ba²⁺、Cr³⁺、Ca²⁺等)进行离子交换，使多个G单元与多价金属阳离子相互交联组成“蛋盒”模型，形成含金属离子的海藻酸钠基水凝胶。同时，高分子链上的双键官能团使其可在光引发剂存在下发生光交联反应，从而构建双交联网络水凝胶，提高水凝胶机械性能。
典型应用
1、弹性组织粘合剂制备
2、生物打印墨水制备：生物打印心脏模型用于药物筛选和疾病建模等
3、污水处理
4、制备水凝胶微球用作胃靶向药物载体
产品优势
1、批次稳定：采用标准化的生产工艺，保证不同批次的AlgMA在成分、理化特性和生物学方面具有高度的重复性和可控性。
2、超高纯度：采用独特的生产工艺，有效地去除甲基丙烯酸小分子和工艺杂质，实现超低杂质水平，保证AlgMA在生物医学应用的安全性。
3、可靠的安全性：每批产品均经过严格的标准化的检验后放行，保证AlgMA在生物医学应用的安全性。
4、高稳定性：参照《无源植入性医疗器械稳定性研究指导原则》，经过实时稳定性和加速稳定性试验证明：AlgMA产品在标示贮存条件下质量稳定。
AlgMA is a photosensitive polymer material derived from the modification of sodium alginate (Alg) with methacrylic anhydride (MA). It can be used in conjunction with blue light or UV photoinitiators and undergoes crosslinking and curing under blue light or ultraviolet irradiation. This polymer features excellent biocompatibility and facile curing conditions. It possesses a unique ability to chelate multivalent cations and exhibits high viscosity. Currently, it has been widely applied in 2D/3D cell culture, bioprinting, drug delivery, tissue engineering scaffolds, cell microencapsulation for immunoisolation, and other fields.
Product Specifications
| Component | Appearance | Specification | Remarks | AlgMA
| White to pale yellow powder or granules
| 1 g/bottle, 5 g/bottle | Store in the dark
|
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Suggestions for Use
1. AlgMA can be used in combination with o-nitrobenzyl alcohol-modified polymers, such as PEGNB, HANB, and GelNB. Both can be polymerized and crosslinked by photoinitiators LAP or NAP under light irradiation, and high-strength hydrogel materials can be obtained within seconds.
2. AlgMA can be used in combination with methacryl-modified polymers (such as CMCMA, GelMA, CSMA, ChMA) and allyl-modified polymers (such as F127DA). Polymerization is initiated by photoinitiators under light irradiation to construct hydrogel materials with different physicochemical properties.
3. AlgMA can be used alone, polymerized and crosslinked by photoinitiators under light irradiation.
Preparation Method
1. Preparation of photoinitiator stock solution: Dissolve an appropriate amount of NAP or LAP to prepare a solution with a concentration in the range of 0.1 wt%-0.25 wt%. Store at 2-8 °C away from light.
2. Weigh the required amount of AlgMA into a centrifuge tube, then add the required volume of initiator stock solution into the tube.
3. Stir the sample in the dark at room temperature or shake on an orbital shaker until completely dissolved. If the sample clumps, crush it with a spatula first; if dissolution is slow, heat and stir at 50-60 °C to assist dissolution.
Precautions
1. The higher the concentration of the prepared AlgMA solution, the higher the modulus of the cured gel and the shorter the curing time.
2. The viscosity of the AlgMA solution is related to the viscosity of the raw material sodium alginate and the solution concentration. For low-viscosity AlgMA, the recommended concentration is ≤ 5 wt%; for medium-viscosity AlgMA, the recommended concentration is ≤ 2 wt%.
3. The higher the concentration of the photoinitiator stock solution, the faster the curing speed of the AlgMA solution.
Applications
3D bioprinting, tumor model construction, cell loading, wound dressings, drug controlled release, dental impressions, etc.
Sterilization Methods
1. Filter sterilization (recommended): Sterilize the solution using a 0.22 μm sterile syringe filter. This method is only suitable for low-viscosity solutions. For high-viscosity solutions, pasteurization or moist heat sterilization is recommended.
2. Pasteurization: Heat the solution to 80 °C and hold for 30 min, then quickly cool to room temperature in an ice-water mixture. Repeat the cycle three times.
3. Moist heat sterilization: Sterilize the solution in an autoclave at 121 °C for 8 min. After manual pressure release, quickly cool the solution to room temperature in an ice-water mixture. Note: High temperature may reduce hydrogel performance; strictly follow the above procedure when using this method.
Note: Sterile solutions can be stored temporarily at 2-8 °C away from light and are recommended to be used within 7 days; Non-sterile solutions can be stored temporarily at 2-8 °C away from light and are recommended to be used within 48 hours.
Features
Na⁺ in the G-units of sodium alginate can undergo ion exchange with multivalent metal cations (Zn²⁺, Fe³⁺, Ba²⁺, Cr³⁺, Ca²⁺, etc.) in aqueous solution, allowing multiple G-units to crosslink with multivalent cations to form an “egg-box” structure, producing metal-ion-containing sodium alginate-based hydrogels.Meanwhile, double bond functional groups on the polymer chains enable photocrosslinking in the presence of photoinitiators, forming a dual-crosslinked network hydrogel with improved mechanical properties.
Typical Applications
1. Preparation of elastic tissue adhesives
2. Preparation of bioprinting inks: Bioprinted cardiac models for drug screening, disease modeling, etc.
3. Wastewater treatment
4. Preparation of hydrogel microspheres as gastric-targeted drug carriers
Advantages
1. Batch-to-batch stability: Standardized production ensures high repeatability and consistency in composition, physicochemical properties, and biological performance among different batches of AlgMA.
2. Ultra-high purity: Unique production process effectively removes methacrylic acid monomers and process impurities, achieving ultra-low impurity levels and ensuring safety for biomedical applications.
3. Reliable safety: Each batch is released after strict standardized testing to guarantee safety in biomedical use.
4. High stability: In accordance with the Guidelines for Stability Study of Non-Invasive Implantable Medical Devices, real-time and accelerated stability tests confirm that AlgMA remains stable under labeled storage conditions.
ZJ26F0636564
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