硫酸软骨素 (CS)是一类硫酸化糖胺聚糖，糖链由交替的葡糖醛酸和N-乙酰半乳糖胺二糖单元组成，存在于结缔组织的细胞外基质中，例如骨，软骨，皮肤，韧带和肌腱，具有良好的生物相容性和可生物降解的特性。CS表现出抗氧化、抗血栓、抗炎和免疫调节作用，可用于骨关节炎、皮肤伤口、中枢神经系统疾病、癌症、心脑血管疾病及眼科疾病的预防和治疗。
CSMA是由硫酸软骨素 (CS)经甲基丙烯酸酐 (MA)修饰得到的、具有光敏特性的高分子材料，可与蓝光或紫外光引发剂配合使用，在蓝光或紫外光辐照下交联固化。该高分子具有优异的生物相容性、可生物降解、抗氧化、抗血栓、抗炎和免疫调节作用，且固化简单，可用于生物打印、细胞2D/3D培养、组织工程器官构建、软骨再生、创伤敷料等领域。
产品规格
| 组分 | 外观 | 规格 | 备注 |
CSMA
| 白色或类白色粉末颗粒
| 1 g/瓶、5 g/瓶 | 避光保存 |
使用建议
1、CSMA可与邻硝基苄醇化高分子，如PEGNB、HANB、GelNB联合使用。二者由光引发剂LAP、NAP光照引发聚合并交联，仅需数秒即可获得高强度水凝胶材料。
2、CSMA可与甲基丙烯基修饰的高分子，如CMCMA、GelMA、HAMA、ChMA以及丙烯基修饰的高分子，如F127DA联合使用，由光引发剂光照引发聚合，构建不同理化性能的水凝胶材料。
3、CSMA单独使用，由光引发剂光照引发聚合并交联。
配制方法
1、配制光引发剂标准液：取一定质量的NAP或LAP配制成浓度为0.1 wt% - 0.25 wt%范围内的溶液，2~8 ℃避光保存；
2、取所需质量的CSMA放入离心管，并取所需体积的引发剂标准溶液加入到上述离心管中；
3、将上述样品于室温下避光搅拌或于摇床振荡，直至完全溶解。样品如在溶液中结块，可先用药匙碾碎；若样品溶解速度偏慢，可置于50-60 ℃环境加热搅拌助溶。
注意事项
1、CSMA配制的浓度越高，固化后形成的凝胶模量越大，固化时间越短。
2、CSMA的建议使用浓度不超过20 wt%。
3、光引发剂标准液浓度越高，CSMA溶液固化速度越快，固化后形成的凝胶越脆。
灭菌方式
1、过滤灭菌 (建议)：使用0.22 μm无菌针头过滤器过滤溶液灭菌，此方法仅限于低粘度溶液使用，如溶液粘度较高时，请选用巴氏灭菌或湿热灭菌法；
2、巴氏灭菌：将溶液加热到80 ℃，保持30 min；再迅速转移至冰水混合物中冷却至常温。共循环上述操作三次；
3、热灭菌：将溶液于高温高压灭菌锅内，121 ℃下灭菌8 min，手动放气后，将溶液迅速转移至冰水混合物中冷却至常温。需注意，高温条件会导致凝胶性能下降，因此使用该方法灭菌时应严格遵守上述操作步骤。
产品应用
1、生物打印、细胞2D/3D培养、组织工程器官构建、软骨再生、创伤敷料等。
2、构建多孔管道支架材料
产品优势
1、批次稳定：采用标准化的生产工艺，保证不同批次的CSMA在成分、理化特性和生物学方面具有高度的重复性和可控性。
2、超高纯度：采用独特的生产工艺，有效地去除甲基丙烯酸小分子和工艺杂质，实现超低杂质水平，保证CSMA在生物医学应用的安全性。
3、可靠的安全性：每批产品均经过严格的标准化的检验后放行，保证CSMA在生物医学应用的安全性。
4、高稳定性：参照《无源植入性医疗器械稳定性研究指导原则》，经过实时稳定性和加速稳定性试验证明：CSMA产品在标示贮存条件下质量稳定。
Chondroitin Sulfate (CS) is a type of sulfated glycosaminoglycan. Its carbohydrate chain consists of alternating disaccharide units of glucuronic acid and N-acetylgalactosamine. It exists in the extracellular matrix of connective tissues, such as bone, cartilage, skin, ligaments, and tendons, and exhibits excellent biocompatibility and biodegradability. CS demonstrates antioxidant, antithrombotic, anti-inflammatory, and immunomodulatory effects, and can be used in the prevention and treatment of osteoarthritis, skin wounds, central nervous system diseases, cancer, cardiovascular and cerebrovascular diseases, and ophthalmic diseases.
CSMA is a photosensitive polymer material obtained by modifying chondroitin sulfate (CS) with methacrylic anhydride (MA). It can be used in combination with blue light or UV photoinitiators and undergoes cross-linking and curing under blue light or UV irradiation. This polymer possesses excellent biocompatibility, biodegradability, antioxidant, antithrombotic, anti-inflammatory, and immunomodulatory properties, with simple curing conditions. It can be applied in fields such as bioprinting, 2D/3D cell culture, tissue engineering and organ construction, cartilage regeneration, and wound dressings.
Product Specifications
| Component | Appearance | Specification | Remarks |
CSMA
| White or off-white powder or granules
| 1 g/bottle, 5 g/bottle | Store in the dark |
Instructions for Use
1. CSMA can be used in combination with o-nitrobenzyl alcohol-modified polymers such as PEGNB, HANB, and GelNB. Polymerization and cross-linking are initiated by photoinitiators LAP or NAP under light irradiation, and high-strength hydrogel materials can be obtained within seconds.
2. CSMA can be used in combination with methacryl-modified polymers (e.g., CMCMA, GelMA, HAMA, ChMA) and allyl-modified polymers (e.g., F127DA). Polymerization is initiated by photoinitiators under light irradiation to fabricate hydrogel materials with diverse physicochemical properties.
3. CSMA can be used alone, with polymerization and cross-linking initiated by photoinitiators under light irradiation.
Preparation Method
1. Preparation of photoinitiator stock solution: Dissolve an appropriate amount of NAP or LAP to prepare a solution with a concentration ranging from 0.1 wt% to 0.25 wt%. Store at 2-8 °C away from light.
2. Weigh the required amount of CSMA into a centrifuge tube, then add the required volume of the initiator stock solution into the tube.
3. Stir the sample in the dark at room temperature or shake on an orbital shaker until completely dissolved. If the sample clumps in the solution, crush it with a spatula first; if dissolution is slow, heat and stir at 50-60 °C to assist dissolution.
Precautions
1. The higher the concentration of the prepared CSMA solution, the greater the modulus of the cured gel and the shorter the curing time.
2. The recommended working concentration of CSMA shall not exceed 20 wt%.
3. The higher the concentration of the photoinitiator stock solution, the faster the curing speed of the CSMA solution, and the more brittle the cured gel.
Sterilization Methods
1. Filter sterilization (recommended): Sterilize the solution using a 0.22 μm sterile syringe filter. This method is only applicable to low-viscosity solutions. For high-viscosity solutions, pasteurization or moist heat sterilization is recommended.
2. Pasteurization: Heat the solution to 80 °C and hold for 30 min, then quickly cool to room temperature in an ice-water bath. Repeat the cycle three times.
3. Moist heat sterilization: Sterilize the solution in an autoclave at 121 °C for 8 min. After manual pressure release, quickly cool the solution to room temperature in an ice-water bath.
Note: High temperatures may degrade gel performance; strictly follow the above procedure when using this sterilization method.
Product Application
1. Bioprinting, 2D/3D cell culture, tissue engineering and organ construction, cartilage regeneration, wound dressings, etc.
2. Fabrication of porous tubular scaffold materials.
Product Advantages
1. Batch-to-batch consistency: Standardized manufacturing ensures high repeatability and controllability of composition, physicochemical properties, and biological performance among different CSMA batches.
2. Ultra-high purity: Unique production process effectively removes small-molecule methacrylic acid and process impurities, achieving an ultra-low impurity level and ensuring safety for biomedical applications.
3. Reliable safety: Each batch is released after strict standardized testing to guarantee safety in biomedical applications.
4. High stability: In accordance with the Guidelines for Stability Studies of Non-Invasive Implantable Medical Devices, real-time and accelerated stability tests confirm that CSMA remains stable under labeled storage conditions.
ZJ26F0434416
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