BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转氢酶（TH）位于线粒体的内膜上，又称为线粒体复合体六，催化 NADH+NADP⁺和 NAD⁺+NADPH 相互转化，调节线粒体NAD(H)和 NADP(H)平衡。把正向反应称为 TH-1，线粒体 NADH 含量增加时会导致线粒体膜的 H⁺ 电化学梯度升高，因而促进了电子传递链上 ROS 的产生。TH-1 促进 NADH 转换为 NADPH，从而提高线粒体的抗氧化能力。该产品可检测动植物组织生物样本。在该试剂盒中，NADH 和 NADPH 均在 340 nm 有特征吸收，因此TH催化的转氢反应不能导致 340 nm 吸光度发生变化。用人工合成底物 3-乙酰吡啶腺嘌呤二核苷酸（APADP⁺）替代NADP⁺，TH-2 催化APADP⁺还原生成 APADPH，APADPH 在 375 nm 有特征光吸收，测定 375 nm 光吸收的增加速率，来计算TH-1 活性。
Transhydrogenase (TH) is located on the inner mitochondrial membrane, also known as mitochondrial Complex VI. It catalyzes the interconversion between NADH + NADP⁺ and NAD⁺ + NADPH, regulating the balance of NAD(H) and NADP(H) in mitochondria. The forward reaction is termed TH-1. An increase in mitochondrial NADH content leads to an elevation of the H⁺ electrochemical gradient across the inner mitochondrial membrane, thereby promoting the production of ROS on the electron transport chain. TH-1 facilitates the conversion of NADH to NADPH, thus enhancing the antioxidant capacity of mitochondria. This product is suitable for detecting biological samples from animal and plant tissues. In this kit, both NADH and NADPH have characteristic absorption at 340 nm; therefore, the transhydrogenation reaction catalyzed by TH does not result in a change in absorbance at 340 nm. Using the synthetic substrate 3-acetylpyridine adenine dinucleotide phosphate (APADP⁺) as a substitute for NADP⁺, TH-1 catalyzes the reduction of APADP⁺ to generate APADPH, which has a characteristic light absorption at 375 nm. The TH-1 activity is calculated by measuring the rate of increase in absorbance at 375 nm.
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