计算溶液所需的质量、体积或浓度。
β-甘露糖苷酶(EC 3.2.1.25, β-Man)属于外切水解酶类,又称 β-D-露糖苷甘露糖水解酶、外切-β-D-甘露聚糖酶,它能够催化结合甘露寡聚糖末端非还原性的 β-1,4-D-糖苷键,并切下一个甘露糖分子,是甘露聚糖完全降解所必需的水解酶。 β-甘露糖苷酶(β-Man)催化对硝基苯酚-β-D-吡喃甘露糖苷产生对硝基苯酚(PNP),该产物在405nm处有特征吸收峰,通过测定405nm光吸收增加速率,即可计算β-甘露糖苷酶活性。
β-Mannosidase (EC 3.2.1.25, β-Man) belongs to the class of exo-hydrolases. It is also known as β-D-mannoside mannohydrolase or exo-β-D-mannanase. This enzyme catalyzes the cleavage of non-reducing terminal β-1,4-D-glycosidic bonds in manno-oligosaccharides, releasing a single mannose molecule. It is an essential hydrolase for the complete degradation of mannan. β-Mannosidase (β-Man) catalyzes the hydrolysis of p-nitrophenyl-β-D-mannopyranoside to produce p-nitrophenol (PNP), which has a characteristic absorption peak at 405 nm. The β-mannosidase activity can be calculated by measuring the increase rate of absorbance at 405 nm.