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1/2 MS培养基 (含琼脂、蔗糖, 39.45g/L)
有货
为什么选择此级别
BioReagent,适用于植物细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
1/2 MS 培养基是植物组织培养中应用极为广泛的改良型基础培养基，在标准 MS 培养基配方基础上，将大量元素无机盐浓度减半，完整保留原配方微量元素、铁盐、维生素、甘氨酸等核心有机与微量营养组分，同时配比添加蔗糖与琼脂，为植物离体培养提供温和、均衡的生长营养环境。广泛用于植物无菌苗培育、组培苗生根培养、幼苗壮苗复壮、茎段/芽体离体培养、原生质体与花药组织培养等，是植物生根培养阶段的主流专用培养基。
产品组分：
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使用方法
1、配制溶解：精确称取本品39.45 g，加入1 L蒸馏水或去离子水中，充分搅拌至粉末完全溶解，配制成均匀的培养基溶液。
2、pH调节（可选）：若需调节pH值，可在灭菌前使用稀NaOH或者稀HCl溶液进行微调。植物组织培养常用pH范围为5.5～5.8，具体参照实验方案执行。调节时需逐滴添加、边加边搅拌并实时检测pH，避免过度反复调节。
3、灭菌处理：将配制好的培养基分装至合适培养容器，于116 ℃高压蒸汽灭菌30 min。
4、冷却使用：灭菌后置于洁净无菌环境自然冷却至室温，全程保持无菌操作，即可用于植物组织培养实验。
注意事项
1、本品为植物组织培养全无机盐培养基，建议采用室温水溶解后再进行高压灭菌，不建议加热溶解；灭菌后溶液若出现少量沉淀为正常现象，不影响实验使用。
2、产品需置于室温、避光环境密封保存，有效期3年。
3、本产品仅供科研实验使用，严禁用于医药、临床诊断与治疗、食品、化妆品等相关领域，请勿存放于普通住宅区。
4、因用户非正常操作、违规使用本产品所产生的一切意外与损失，本公司不承担相关责任。
1/2 MS medium is a widely used modified basal medium for plant tissue culture. Based on the standard MS medium formula, the concentration of macroelement inorganic salts is halved, while core organic and micro-nutrient components including trace elements, iron salts, vitamins and glycine from the original formula are fully retained. Sucrose and agar are added in matched proportions to provide a mild and balanced nutritional environment for the growth of in vitro plant cultures.It is extensively applied in sterile seedling cultivation, rooting culture of tissue-cultured seedlings, seedling hardening and rejuvenation, in vitro culture of stem segments and buds, protoplast culture, anther tissue culture and other procedures, serving as the mainstream dedicated medium for plant rooting stages.
Product Composition：
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Instructions for Use
1. Preparation and Dissolution: Accurately weigh 39.45 g of this product, add it to 1 L of distilled or deionized water, and stir thoroughly until the powder dissolves completely to prepare a homogeneous medium solution.
2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5～5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.
3. Sterilization Treatment: Dispense the prepared medium into appropriate culture vessels, then autoclave at 116 °C under high-pressure steam for 30 minutes.
4. Cooling and Application: After sterilization, let the medium cool naturally to room temperature in a clean sterile environment, mix gently, and maintain aseptic technique throughout all operations. The medium is then ready for plant tissue culture experiments.
Precautions：
1. This product is a full inorganic salt medium for plant tissue culture. It is recommended to dissolve the powder in room-temperature water prior to autoclaving; heating for dissolution is not advised. Minor precipitation after sterilization is normal and will not interfere with experimental results.
2. Store the product hermetically sealed at room temperature away from light. The shelf life is 3 years.
3. This product is for research laboratory use only. It is strictly prohibited for medical purposes, clinical diagnosis and treatment, food, cosmetics and other related fields. Do not store it in residential areas.
4. Our company shall not be liable for any accidents or losses arising from improper operation or unauthorized use of this product by users.
规格
别名
减半 MS 培养基
英文别名
1/2 Murashige&Skoog Medium
规格或纯度
BioReagent, 适用于植物细胞培养
稳定性与储存
Store at room temperature long term (36months). Store in the dark.
英文名称
1/2 MS Medium (with agar, sucrose, 39.45g/L)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838778 |
分析证书 | M1524309 |
技术文档和文章
溶液计算器
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