计算溶液所需的质量、体积或浓度。
BioReagent,适用于植物细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
MS 培养基是当前植物组织培养领域应用最为广泛的基础培养基。该培养基具备高浓度无机盐体系,能够充足供给植物离体组织生长发育所需的各类矿质营养,可有效促进愈伤组织的增殖生长,为植物外植体分化、再生提供稳定的营养支撑。 本款培养基为改良型纯无机 MS 基础干粉,严格剔除有机元素、琼脂以及蔗糖,仅保留 MS 标准配方全套大量元素、微量元素与铁盐无机离子体系。配方精准复刻经典 MS 基础盐配比,无各类维生素等有机添加物,无外源碳源与凝固基质,基质背景极度干净,最大程度规避外源杂质对植物胁迫实验、精准组培实验的干扰。
产品成分:
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使用方法
1、配制溶解:精确称取本品4.63 g,加入1 L蒸馏水或去离子水中,充分搅拌至粉末完全溶解,配制成均匀的培养基溶液。
2、pH调节(可选):若需调节pH值,可在灭菌前使用稀NaOH或者稀HCl溶液进行微调。植物组织培养常用pH范围为5.5~5.8,具体参照实验方案执行。调节时需逐滴添加、边加边搅拌并实时检测pH,避免过度反复调节。
3、灭菌处理:将配制好的培养基分装至合适培养容器,于116 ℃高压蒸汽灭菌30 min。
4、冷却使用:灭菌后置于洁净无菌环境自然冷却至室温,全程保持无菌操作,即可用于植物组织培养实验。
注意事项
1、本品为植物组织培养全无机盐培养基,建议采用室温水溶解后再进行高压灭菌,不建议加热溶解;灭菌后溶液若出现少量沉淀为正常现象,不影响实验使用。
2、产品需置于室温、避光环境密封保存,有效期3年。
3、本产品仅供科研实验使用,严禁用于医药、临床诊断与治疗、食品、化妆品等相关领域,请勿存放于普通住宅区。
4、因用户非正常操作、违规使用本产品所产生的一切意外与损失,本公司不承担相关责任。
MS medium is the most widely used basal medium in the field of plant tissue culture. Featuring a high-concentration inorganic salt system, this medium fully supplies all mineral nutrients required for the growth and development of isolated plant tissues. It can effectively boost callus proliferation and growth, and provides stable nutritional support for the differentiation and regeneration of plant explants.The inorganic nutrient components of MS medium are scientifically formulated with balanced proportions, which perfectly match the nutritional demands and physiological characteristics of plant cell growth. For routine plant tissue culture experiments, no extra organic additives such as amino acids, casein hydrolysate, yeast extract or coconut water are required, as the basal nutrients can satisfy the in vitro culture demands of the vast majority of plant species.In terms of application scenarios, solid MS medium has versatile uses: it can be applied for callus induction and subculture, and is compatible with the in vitro cultivation of various plant explants including embryos, stem segments, shoot tips and anthers. The corresponding liquid MS medium is directly used for plant cell suspension culture; it efficiently sustains normal cellular growth and metabolism, delivering excellent culture success rate and stability.
Product Components:
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Instructions for Use:
1. Preparation and Dissolution: Accurately weigh 4.63 g of this product, add it to 1 L of distilled or deionized water, and stir thoroughly until the powder dissolves completely to prepare a homogeneous medium solution.
2. pH Adjustment (Optional): If pH adjustment is required, finely tune the pH with dilute NaOH or dilute HCl solution before sterilization. The commonly applicable pH range for plant tissue culture is 5.5~5.8, subject to specific experimental protocols. Add the solution dropwise while stirring and monitor the pH in real time to avoid over-adjustment.
3. Sterilization Treatment: Dispense the prepared medium into appropriate culture vessels, then autoclave at 116 °C under high-pressure steam for 30 minutes.
4. Cooling and Application: After sterilization, let the medium cool naturally to room temperature in a clean sterile environment, mix gently, and maintain aseptic technique throughout all operations. The medium is then ready for plant tissue culture experiments.
Precautions:
1. This product is a full inorganic salt medium for plant tissue culture. It is recommended to dissolve the powder in room-temperature water prior to autoclaving; heating for dissolution is not advised. Minor precipitation after sterilization is normal and will not interfere with experimental results.
2. Store the product hermetically sealed at room temperature away from light. The shelf life is 3 years.
3. This product is for research laboratory use only. It is strictly prohibited for medical purposes, clinical diagnosis and treatment, food, cosmetics and other related fields. Do not store it in residential areas.
4. Our company shall not be liable for any accidents or losses arising from improper operation or unauthorized use of this product by users.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | M1524318 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for plant cell culture 级别指南 →