计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,用于细胞培养,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
支原体染色液 (DNA荧光染色法) 是用于在培养细胞中原位染色检测支原体或其它原核生物的试剂。主要用于检测培养细胞中是否存在支原体污染。
培养细胞中的细菌污染、酵母污染或霉菌污染都在光学显微镜下可见,但支原体污染在光学显微镜下不可见,必须通过特定的方法进行检测。检测支原体污染的方法包括支原体分离培养、支原体特异酶检测、RT-PCR检测以及DNA荧光染色检测。本品是通过DNA荧光染色来检测支原体的。可快速、有效、高灵敏度地检测支原体污染。
操作步骤:
1. 按2滴/ml培养基的比例滴加支原体检测染色液。
2. 室温孵育15-30分钟。
3. 荧光显微镜观察 (波长:360~400nm)。
案例分析:

1. 无污染 2. 轻度污染 3. 严重污染
本试剂盒的检测效果图参考上图。
图1. 为无支原体污染情况,图2. 为支原体轻度污染情况,箭头所指为微粒状的一些支原体,图3. 为支原体重度污染情况,可见大量微粒状的支原体。
注意事项:
1、本染色试剂对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。
2、荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
3、本产品仅限于专业人员的科学研究用,为了您的安全和健康,请穿实验服并戴手套操作。
Mycoplasma Staining Solution (DNA Fluorescent Staining Method) is a reagent for in‑situ staining to detect mycoplasma or other prokaryotes in cultured cells. It is mainly used to verify mycoplasma contamination in cultured cells.
Bacterial, yeast and mold contamination in cultured cells can be observed under an optical microscope. However, mycoplasma contamination cannot be visualized under an optical microscope and must be examined by dedicated methods. Common mycoplasma detection approaches include mycoplasma isolation and culture, mycoplasma‑specific enzyme assay, RT‑PCR assay, and DNA fluorescent staining. This product detects mycoplasma via DNA fluorescent staining, enabling rapid, effective and highly‑sensitive identification of mycoplasma contamination.
Operating Procedures:
1. Add Mycoplasma Detection Staining Solution at a ratio of 2 drops per mL of culture medium.
2. Incubate at room temperature for 15‑30 minutes.
3. Observe under a fluorescence microscope (wavelength: 360~400 nm).
Case Analysis:

1. No contamination 2. Mild contamination 3. Severe contamination
Refer to the figure above for reference detection results of this kit.
Figure 1 shows the condition without mycoplasma contamination. Figure 2 shows mild mycoplasma contamination; particulate mycoplasma particles are indicated by arrows. Figure 3 shows severe mycoplasma contamination, where abundant particulate mycoplasma can be observed.
Precautions:
1. This staining reagent is hazardous to human body. Handle with caution and take adequate protective measures to avoid direct physical contact or inhalation.
2. Fluorescent dyes are susceptible to photobleaching. It is recommended to complete detection on the same day after staining.
3. This product is for scientific research use by professionals only. Wear a lab coat and gloves during operation for your safety and health.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 → 查看 for Cell culture 级别指南 →