计算溶液所需的质量、体积或浓度。
EnzymoPure™,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Xpa6Man是一种α-1,6-甘露糖苷酶,基因来源于Xanthomonas phaseoli,可用于各种糖链非还原端为α1,6连接甘露糖的水解。本产品为大肠杆菌重组表达。
反应式

使用方法
1.按照反应体系加入底物,可适当加入缓冲盐调节pH为6.0,加入适量Xpa6Man(根据反应需求);
2.混匀后,将样品置于37℃孵育4h或者过夜。
【注】体系放大时,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
【注】多数常见的生物缓冲液适用,比如Tris或PBS,不在此pH范围的缓冲液(如醋酸缓冲液)可能适用,但是孵育时间或酶量需要根据实际情况进行优化。
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
Xpa6Man is an α‑1,6‑mannosidase whose gene is derived from Xanthomonas phaseoli. It can be used for the hydrolysis of various glycan chains that have an α1,6‑linked mannose at their non‑reducing end. This product is recombinantly expressed in E. coli.
Reaction Scheme

Usage Method
1.Add the substrate according to the reaction system. An appropriate buffer salt may be added to adjust the pH to 6.0, and then add an appropriate amount of Xpa6Man (depending on the reaction requirements).
2.After mixing, incubate the sample at 37 °C for 4 hours or overnight.
Notes
1.When scaling up the reaction system, the incubation time or enzyme amount should be adjusted according to the actual conditions; low‑speed shaking may be used to ensure mixing.
2.Most common biological buffers (e.g., Tris or PBS) are suitable. Buffers outside this pH range (such as acetate buffer) may also be applicable, but the incubation time or enzyme amount may need to be optimized according to the actual situation.
Precautions
For your safety and health, please wear a lab coat and disposable gloves when handling. This product is for research use only.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | M1525150 | |
| 分析证书 | M1525150 |
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