计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
M199培养液起初开发用于鸡胚成纤维细胞的营养研究。它具有广泛的物种适用性,特别适用于非转化细胞的培养。 M199培养液广泛用于病毒学、疫苗生产以及小鼠胰腺上皮和大鼠晶状体组织原代外植体的体外培养。与其他基础培养基相比,M199培养液包含独特的组分,包括腺嘌呤、腺苷、次黄嘌呤、胸腺嘧啶和其他维生素。M199培养液包含 Earle's 盐,用于 CO₂ 培养箱,或包含 Hanks' 盐,用于无 CO₂ 培养箱。本产品为M199培养液 (10×) ,通常是标准工作浓度的10倍。在使用前需要将其稀释到适当的工作浓度,通常是通过与去离子水或无菌蒸馏水混合来实现。这种浓缩形式有助于减少储存空间,并且可以降低运输成本。本品不含蛋白、脂质或生长因子,内毒素≤0.5 EU /mL。
组成成分
| Components | Concentration (mg/L) | mM |
| L‑谷氨酰胺 | 1g/L | 6.8mM |
| D‑葡萄糖 | 10g/L | 55.6mM |
| 丙酮酸钠 | - | - |
| HEPES | - | - |
| 酚红 | 200mg/L | 0.5mM |
| NaHCO₃ | 2200mg/L | 26.19mM |
注意事项
1. 本产品经过0.1μm 过滤除菌,使用时应注意无菌操作,避免污染;
2. 为保持本产品的最佳使用效果,请勿进行冻融处理;
3. 本产品仅用于科研或进一步研究使用,不用于诊断和治疗。
Medium 199 was originally developed for nutritional studies on chick embryo fibroblasts. It has broad‑species applicability and is particularly suitable for the culture of non‑transformed cells. Medium 199 is widely used in virology, vaccine production, and the in‑vitro culture of primary explants derived from mouse pancreatic epithelium and rat lens tissue. Compared with other basal media, this Medium 199 contains unique components including adenine, adenosine, hypoxanthine, thymidine and other vitamins. Medium 199 is available with Earle’s salts for use in a CO₂ incubator or Hanks’ salts for CO₂‑free incubator conditions. This product is Medium 199 (10×), normally ten‑fold of the standard working concentration. It shall be diluted to the appropriate working concentration prior to use, generally by mixing with deionized water or sterile distilled water. This concentrated form helps save storage space and reduce transportation costs. This product contains no proteins, lipids or growth factors, with endotoxin ≤0.5 EU/mL.
Composition
| Components | Concentration (mg/L) | mM |
| L-glutamine | 1g/L | 6.8mM |
| D-glucose | 10g/L | 55.6mM |
| Sodium pyruvate | - | - |
| HEPES | - | - |
| Phenol red | 200mg/L | 0.5mM |
| NaHCO₃ | 2200mg/L | 26.19mM |
Precautions
1. This product is sterile‑filtered through a 0.1 μm membrane. Aseptic manipulation shall be performed during use to avoid contamination.
2. To maintain optimal performance of this product, freeze‑thaw cycles are prohibited.
3. This product is intended solely for scientific research and further investigation; it shall not be used for diagnostic or therapeutic purposes.
| pH | 7.1-7.4 |
|---|
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