计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-甘露聚糖酶(EC 3.2.1.78)广泛存在于动植物及微生物中,在饲料工业中应用广泛,不仅可消除饲料中的抗营养因子甘露聚糖、提高饲料利用率,还能促进有益菌增殖,增强动物免疫功能。本试剂盒采用3,5-二硝基水杨酸(DNS)比色法测定该酶活性:β-甘露聚糖酶水解甘露聚糖生成还原性寡糖和单糖,后者在沸水浴条件下与DNS试剂发生氧化还原反应,生成在540 nm处具有特征吸收峰的棕红色氨基化合物。通过测定该波长下吸光值的增幅,即可精准计算样品中β-甘露聚糖酶的活力大小。
β-Mannanase (EC 3.2.1.78) is widely distributed in animals, plants, and microorganisms, and has broad applications in the feed industry. It not only eliminates the anti-nutritional factor mannan in feed and improves feed utilization, but also promotes the proliferation of beneficial bacteria and enhances animal immune function. This kit employs the 3,5-dinitrosalicylic acid (DNS) colorimetric method for the determination of enzyme activity: β-Mannanase hydrolyzes mannan to generate reducing oligosaccharides and monosaccharides, which undergo an oxidation-reduction reaction with DNS reagent under boiling water bath conditions to form a brownish-red amino compound with a characteristic absorbance peak at 540 nm. By measuring the increase in absorbance at this wavelength, the β-mannanase activity in the sample can be precisely calculated.