BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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氧自由基攻击脂质中的不饱和脂肪酸，生成过氧化脂质；过氧化脂质进一步分解为丙二醛（MDA）等一系列复杂产物。因此，通过检测MDA的含量可反映样本的脂质过氧化水平。本试剂盒的检测原理为：在酸性和高温条件下，MDA与硫代巴比妥酸（TBA）发生缩合反应，生成棕红色的三甲川（3,5,5-三甲基恶唑-2,4-二酮），该产物在532 nm处具有最大吸收峰。同时测定600 nm处的吸光度以消除蔗糖的干扰，利用532 nm与600 nm吸光度差值计算样本中MDA的含量。
Oxygen free radicals attack unsaturated fatty acids in lipids, generating lipid peroxides, which are further decomposed into a series of complex products, including malondialdehyde (MDA). Therefore, the MDA content can be used to reflect the level of lipid peroxidation in a sample. The principle of this kit is based on the condensation reaction between MDA and thiobarbituric acid (TBA) under acidic and high-temperature conditions, forming a brownish-red trimethine chromophore (3,5,5-trimethyloxazolidine-2,4-dione), which exhibits a maximum absorbance peak at 532 nm. Simultaneously, the absorbance at 600 nm is measured to correct for interference from sucrose. The MDA content in the sample is calculated from the difference in absorbance between 532 nm and 600 nm.
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