BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
线粒体呼吸链复合体V（ATP合成酶，F₁F₀-ATP酶）是氧化磷酸化的终末酶，负责合成细胞所需的大部分ATP，其功能异常与心肌及神经系统疾病密切相关。本试剂盒采用偶联酶法测定该酶活性：复合体V催化ADP与Pi生成ATP，ATP经己糖激酶和G6PDH偶联反应将NADP⁺还原为NADPH，于340 nm处测定NADPH生成速率，其与复合体V活力成正比，据此定量酶活性。
Mitochondrial respiratory chain complex Ⅴ (ATP synthase, F₁F₀-ATPase) is the terminal enzyme of oxidative phosphorylation, responsible for synthesizing the majority of ATP required by cells. Its dysfunction is closely associated with myocardial and neurological diseases. This kit employs a coupled enzyme method for the determination of this enzyme activity. The principle is as follows: Complex Ⅴ catalyzes the formation of ATP from ADP and inorganic phosphate (Pi). The generated ATP subsequently drives a coupled reaction system consisting of hexokinase (HK) and glucose‑6‑phosphate dehydrogenase (G6PDH), which reduces NADP⁺ to NADPH. The rate of NADPH production is monitored at 340 nm. This rate is directly proportional to the complex Ⅴ activity, allowing for quantitative determination of the enzyme activity accordingly.
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