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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
线粒体复合体Ⅴ，又称F₁F₀-ATP合酶，广泛存在于动物、植物、微生物及培养细胞的线粒体中，由F₁和F₀两个亚基组成。该酶利用呼吸链产生的质子电化学梯度催化ATP合成，也可逆水解ATP。此外，复合体Ⅴ还存在于叶绿体、异养菌和光合细菌中，是线粒体氧化磷酸化和叶绿体光合磷酸化合成ATP的关键酶。本试剂盒的测定原理为：复合体Ⅴ水解ATP生成ADP和无机磷（Pi），通过测定Pi的生成速率，即可定量计算复合体Ⅴ的酶活性。
Mitochondrial complex V, also known as F₁F₀-ATP synthase, is widely distributed in the mitochondria of animals, plants, microorganisms and cultured cells, and consists of two subunits, F₁ and F₀. This enzyme utilizes the proton electrochemical gradient generated by the respiratory chain to catalyze ATP synthesis, and can also reversibly hydrolyze ATP. In addition, complex V is also found in chloroplasts, heterotrophic bacteria and photosynthetic bacteria, serving as a key enzyme responsible for ATP synthesis via mitochondrial oxidative phosphorylation and chloroplast photophosphorylation. The detection principle of this kit is as follows: complex V hydrolyzes ATP to produce ADP and inorganic phosphorus (Pi). The enzymatic activity of complex V can be quantitatively calculated by measuring the production rate of Pi.
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