BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
柠檬酸（CA）是线粒体三羧酸循环（TCA循环）的第一个中间产物，由柠檬酸合酶催化乙酰CoA与草酰乙酸缩合而成，其含量是衡量TCA循环强度的核心指标之一。本试剂盒可配合丙酮酸含量、丙酮酸脱氢酶（PDH）活性、乙酰CoA含量及柠檬酸合酶（CS）活性的测定，进行系统性的代谢分析：（1）丙酮酸含量与PDH活性变化可反映糖酵解途径的进行程度；（2）综合丙酮酸、PDH及乙酰CoA的数据，可评估脂肪酸β-氧化途径对乙酰CoA的供给情况；（3）整合乙酰CoA含量、CS活性及CA含量，可全面反映TCA循环的运转状况。本试剂盒的测定原理为：CA在柠檬酸裂解酶作用下生成α-酮酸（草酰乙酸）；在弱酸性条件下，α-酮酸与苯肼反应生成对应的α-酮酸苯腙，该产物在330 nm处具有特征吸收峰。通过测定该波长下吸光度的变化，即可定量计算样品中CA的含量。
Citric acid (CA) is the first intermediate product of the mitochondrial tricarboxylic acid (TCA) cycle, synthesized by the condensation of acetyl‑CoA and oxaloacetate catalyzed by citrate synthase. Its content is one of the core indicators for evaluating the intensity of the TCA cycle. This kit can be used in conjunction with the determination of pyruvate content, pyruvate dehydrogenase (PDH) activity, acetyl‑CoA content, and citrate synthase (CS) activity to perform systematic metabolic analysis: ① Changes in pyruvate content and PDH activity reflect the progression of the glycolytic pathway; ② Integrating the data of pyruvate, PDH, and acetyl‑CoA allows for the assessment of the acetyl‑CoA supply from the fatty acid β‑oxidation pathway; ③ Integrating acetyl‑CoA content, CS activity, and CA content provides a comprehensive reflection of the operational status of the TCA cycle. The assay principle of this kit is as follows: CA is converted to α‑keto acid (oxaloacetate) by citrate lyase. Under weakly acidic conditions, the α‑keto acid reacts with phenylhydrazine to form the corresponding α‑keto acid phenylhydrazone, which exhibits a characteristic absorption peak at 330 nm. By measuring the change in absorbance at this wavelength, the CA content in the sample can be quantitatively calculated.
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