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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品用途:用于支原体的培养(中国药典)。 使用方法:称取本品30.5 g,溶于1 L纯化水中,搅拌加热至沸,使完全溶解。分装于适宜容器中,于121 ℃高压灭菌15分钟。灭菌后冷却至约56 ℃,按培养基与灭菌小牛血清体积比8∶2加入血清,并可酌情添加青霉素等抑菌剂。充分摇匀,备用。 配方:/L 猪胃消化物 10.0g 牛肉浸粉 5.0g 酵母浸粉 5.0g 氯化钠 2.5g 磷酸氢二钾 3.3g 葡萄糖 5.0g 琼脂 3.0g PH 7.6±0.2(25℃)
Product application: For the cultivation of Mycoplasma (Chinese Pharmacopoeia). Usage: Weigh 30.5 g of this product, dissolve in 1 L of purified water, stir and heat to boiling until completely dissolved. Dispense into suitable containers and autoclave at 121°C for 15 minutes. After sterilization, cool to approximately 56°C, add serum at a volume ratio of 8:2 (medium:sterile calf serum), and optionally add antibacterial agents such as penicillin. Shake well and set aside for use. Formula: /L Porcine stomach digest 10.0 g Beef extract powder 5.0 g Yeast extract powder 5.0 g Sodium chloride 2.5 g Dipotassium hydrogen phosphate 3.3 g Glucose 5.0 g Agar 3.0 g pH 7.6 ± 0.2 (25°C)