计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
平衡盐溶液(Balanced Salt Solution,BSS)与细胞生长状态下的pH值、渗透压等环境状态一致,具有维持渗透压、控制酸碱平衡、供给细胞生存代谢所必需的能量和无机盐成分等作用,可满足体外实验中细胞生存并维持一定代谢的基本需要。
改良台氏液粉剂也属于平衡盐溶液的一种,主要由氯化钠、磷酸盐、氯化钙、葡萄糖等组成,含HEPES,缓冲能力较常规台式液粉剂(阿拉丁货号:T1529491)强,本产品将氯化钙与其他成分分开存储,使用时分开溶解再混合即可使用,避免因一起加入出现不溶性沉淀的问题,经常用于哺乳机体灌流、清洗组织,并维持离体肠肌的正常生理功能。
该试剂仅用于科研领域,不适用于临床诊断或其他用途。
组分与存储
| 组分货号 | 组分名称 | 物理外观 | 1L | 储存 |
| M1529492A | 改良台式液粉剂 (无钙) | 粉末 | 1EA | 2-8℃. |
| M1529492B | 钙盐溶液 | 液体 | 1EA | 2-8℃. |
操作步骤(仅供参考):
1、一般取1 L包装的改良台氏液粉剂(无钙)充分溶解于800 mL去离子水(1升烧杯),再加入钙盐溶液,混匀并定容至1000 mL,用于保存或清洗离体组织。
2、一般不需要调整pH值,如有需要,可自行调整,4℃保存,6个月有效。
注意事项:
1、由于改良台氏液含钙离子,容易产生沉淀,如果产生少许沉淀,可以加热溶解或过滤后使用,产生大量沉淀应弃用。
2、该试剂含有葡萄糖和HEPES,溶解后保存不当可能长菌,使用时应注意溶液的状态。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Balanced Salt Solution (BSS) matches the pH, osmotic pressure and other environmental conditions under cell‑growth status. It can maintain osmotic pressure, regulate acid‑base balance, and supply energy and inorganic salts essential for cell survival and metabolism, satisfying the basic requirements for cell survival and certain metabolic activities in in‑vitro experiments.
Modified Tyrode's solution powder is one type of balanced salt solution. It is mainly composed of sodium chloride, phosphate, calcium chloride, glucose, etc., and contains HEPES. Its buffering capacity is stronger than that of conventional Tyrode's solution powder (Aladdin Cat. No.: T1529491). In this product, calcium chloride is stored separately from other components. Dissolve them respectively and mix prior to use to avoid insoluble precipitates caused by simultaneous dissolution. It is frequently used for mammalian organ perfusion, tissue rinsing, and maintaining the normal physiological functions of isolated intestinal muscle.
This reagent is for research use only and shall not be used for clinical diagnosis or other purposes.
Contents & Storage
| Component No. | Component Name | Appearance | 1L | Storage |
| M1529492A | Calcium‑free modified Tyrode's solution powder | Powder | 1EA | 2-8℃. |
| M1529492B | Calcium salt solution | Liquid | 1EA | 2-8℃. |
Operating Procedures (For reference only):
1. Fully dissolve 1‑L‑packaged calcium‑free modified Tyrode's solution powder in 800 mL deionized water in a 1‑L beaker. Add calcium salt solution, mix thoroughly and make up the volume to 1000 mL for preservation or rinsing of isolated tissues.
2. pH adjustment is generally unnecessary; adjust pH manually if needed. Valid for 6 months when stored at 4 °C.
Precautions:
1. Precipitates are liable to form due to calcium ions in modified Tyrode’s solution. Minor precipitates can be dissolved by heating or removed by filtration before use. Discard the solution in case of massive precipitates.
2. This reagent contains glucose and HEPES. Bacterial growth may occur if the prepared solution is improperly stored. Check the condition of the solution before use.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.