计算溶液所需的质量、体积或浓度。
BioReagent,用于显微镜 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
M 缓冲液又称微丝染色缓冲液,用于细胞骨架样品的制备,能够有效维持微丝结构的稳定。该缓冲液由咪唑、氯化钾、氯化镁、EGTA、EDTA·2Na、β-巯基乙醇、甘油及蒸馏水配制而成。其中,咪唑作为缓冲组分,负责稳定体系的 pH 环境;EGTA 与 EDTA·2Na 协同螯合游离的 Ca²⁺,同时配方中的氯化镁为体系提供必要的 Mg²⁺。在低钙环境下,该缓冲液有助于维持骨架纤维的聚合与舒展状态,减少微丝解聚,从而适用于微丝染色及后续的显微观测。
操作步骤(仅供参考):
根据实验要求操作。
注意事项:
1、尽量避免污染。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
M Buffer, also known as microfilament staining buffer, is designed for the preparation of cytoskeletal samples and effectively maintains the stability of microfilament structures. It is composed of imidazole, potassium chloride, magnesium chloride, EGTA, EDTA·2Na, β-mercaptoethanol, glycerol, and distilled water. In this formulation, imidazole serves as the buffering component to stabilize the pH of the system; EGTA and EDTA·2Na work synergistically to chelate free Ca²⁺, while magnesium chloride supplies the necessary Mg²⁺. Under low-calcium conditions, this buffer helps maintain the polymerized and extended state of cytoskeletal fibers and reduces microfilament depolymerization, making it suitable for microfilament staining and subsequent microscopic observation.
Operating Procedures (For Reference Only):
Operate in accordance with experimental requirements.
Precautions:
1. Avoid contamination as much as possible.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
| pH | 7.2 |
|---|