计算溶液所需的质量、体积或浓度。
BioReagent,无菌,用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 164 篇同行评审文献引用。
甲基纤维素(Methylcellulose,CAS号9004-67-5),平均分子量1万~22万。本品在无水乙醇、乙醚、丙酮等有机溶剂中几乎不溶;在80~90℃热水中迅速分散、溶胀,降温后溶解,形成澄清或微乳白色溶液。其水溶液具有温敏可逆凝胶特性:高温时形成凝胶,降温后恢复为溶液;并具有优良的润湿性、分散性、粘接性、增稠性和乳化性。
本品为经高压灭菌处理的2×浓缩无菌溶液(1.8%),建议工作浓度为1×。使用时加入少量于悬浮培养基中,或按具体实验要求调整用量,以增加培养基粘稠度。所用甲基纤维素原料在2%浓度下粘度不低于450 cps。主要用于美希索体系中的细胞克隆培养、悬浮培养基配制、细胞集落形成及集落分离等科研实验。
本品仅用于科研领域,不适用于临床诊断或其他用途。
操作步骤(仅供参考):
根据实验具体要求操作。一般工作浓度为 1×。
注意事项:
1、该试剂为无菌溶液,请注意无菌操作。
2、试剂开封后请尽快使用,以防影响后续实验效果。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Methylcellulose (CAS: 9004-67-5), average molecular weight 10,000–220,000. It is practically insoluble in organic solvents such as anhydrous ethanol, diethyl ether and acetone. It disperses and swells rapidly in hot water at 80–90 ℃ and dissolves upon cooling to form a clear or slightly opalescent solution. Its aqueous solution exhibits thermoreversible gelation: it forms a gel at elevated temperature and reverts to liquid upon cooling. It also possesses excellent wettability, dispersibility, adhesiveness, thickening and emulsifying properties.
This product is an autoclaved sterile 2× concentrated solution (1.8%). The recommended working concentration is 1×. Add an appropriate amount into suspension medium or adjust the dosage according to specific experimental requirements to modulate medium viscosity. The viscosity of the methylcellulose raw material is no less than 450 cP at a concentration of 2%. It is mainly used for research experiments such as cell colony culture in Methocult system, preparation of suspension medium, cell colony formation and colony isolation.
For research use only. Not for clinical diagnosis or other applications.
Operating Procedures (For reference only):
Operate according to experimental requirements. The commonly used working concentration is 1×.
Precautions:
1. This is a sterile solution. Aseptic operation is required.
2. Use promptly after opening to avoid adverse effects on subsequent experiments.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
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