计算溶液所需的质量、体积或浓度。
用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 8 篇同行评审文献引用。
MEM培养基(Minimum Essential Medium)也称最低必需培养基、最小基本培养基或低限量Eagle培养基,由Harry Eagle在Eagle基本培养基(BEM)的基础上发展而来的,是一种最基本、适用范围最广的培养基,是动物细胞培养中最常用的培养基之一。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素,成分简单,主要用于贴壁细胞的培养,配方修改后也可用于其他类型细胞培养。
MEM含NEAA(非必须氨基酸)的培养基,是在MEM培养基的基础上添加L-丙氨酸、L-谷氨酸、L-天(门)冬酰胺、L-天(门)冬氨酸、L-脯氨酸、L-丝氨酸和甘氨酸7种NEAA,能降低细胞培养时细胞自身生产非必须氨基酸的副作用,有效促进细胞增殖代谢。
本产品含有多类细胞培养所需的氨基酸、维生素、无机盐等多种成分,但不含蛋白质、脂类或任何生长因子,故此产品需搭配血清或无血清添加物使用。
成分说明:
平衡盐:Earle’s盐
NEAA:含
L-谷氨酰胺:2mM
D-葡萄糖:1000mg/L
HEPES缓冲剂:无
酚红指示剂:10mg/L
制备说明:
【1】 于一容器中倒入约90%超纯水,将本品一袋或者称取 1L 用量,全部倒入该容器中,用少量超纯水将袋内残留粉末洗出。
【2】搅拌30min使所有成分完全溶解,待溶液澄清后,加入2.2g碳酸氢钠(分析纯),继续搅拌5-10min至溶解。
【3】加超纯水定容至1L。
【4】必要时用1mol/L氢氧化钠溶液或1mol/L盐酸溶液调整PH值至7.20-7.30,由于过滤会使培养基PH值稍微偏高,因此此处比目的PH值(7.20-7.40)要低一些。
【5】用孔径为0.2μm的滤膜正压过滤除菌(注意无菌操作)。
【6】过滤结束可以取少许液体培养基进行菌检,待合格后再使用。此时液体培养基保质期为1年,储存条件为2~8℃。
备注:该使用方法以1L规格为例,其他规格使用方法按照对应规格加入对应量的碳酸氢钠以及定容到对应规格的体积即可。
Earle’s salts: yes
NEAA: yes
L-glutamine: 2mM
D-glucose:1000mg/L
HEPES:no
Phenol red: 10mg/L
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