| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| M666092-1ml |
1ml |
期货  |
| |
| M666092-5ml |
5ml |
期货 |
|
| 别名 | 多用途 SNP 基因分型 qPCR MIX | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 英文名称 | Multipurpose SNP Genotyping qPCR MIX | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 储存温度 | -20°C储存,避免反复冻融 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 运输条件 | 超低温冰袋运输 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
产品简介
Multipurpose SNP Genotyping qPCR MIX是针对探针法SNP分型的实时
荧光定量2×PCR预混体系，包括Taq DNA Polymerase、PCR Buffer、dNTPs、Mg²+
以及增强剂和稳定剂，操作简单方便。独特的PCR缓冲体系对血液、唾液等复杂模板
有极强的耐受性，不仅可以对提取后的DNA进行高效扩增，而且支持口腔拭子液和终
浓度不高于15%的血液直接扩增，无需繁杂的提取及保存过程。分型结果快速、准
确。
实验前准备及重要注意事项
1.使用前请上下颠倒轻轻混匀，尽量避免起泡，并经短暂离心后使用。 2.避免反复冻融本品，反复冻融可能使产品性能下降。本产品长期保存可置于-20℃
避光保存。如果在短期内需要频繁使用，可在2-8℃保存。
操作步骤
以初次进行分型的引物为例 1. PCR反应体系
2.以需要分型的不同基因型标准品为模板，分别进行退火温度的优化，从而达到更好 的分型效果。 3.模板处理。血液模板可直接使用ddH2O稀释至不同浓度进行扩增，推荐使用终浓度
2%血液作为模板进行分型扩增；口腔拭子模板，可将拭子在口腔内壁轻轻刮拭6次
左右并置于400 μL—1000 μL ddH2O中振荡混匀后直接用作模板。
4. PCR反应程序 本品可采用两步法PCR反应程序
注意： 1）采用两步法PCR反应程序，若因使用Tm值较低的引物等原因导致信号较低或CT值较 大的情况，可尝试三步法PCR扩增。 2）实时采集信号曲线法分型及最终采集信号终点法分型均可。
Products content
Products Introduction CombiVision Multipurpose SNP Genotyping qPCR MIX is a real-time fluorescence quantitative 2×PCR premix system for SNP typing by probe method, which includes Taq DNA Polymerase, PCR Buffer, dNTPs, Mg²+, as well as enhancers and stabilizers, and is easy to operate. The unique PCR buffer system is highly tolerant to complex templates, such as blood and saliva, and can not only efficiently amplify the extracted DNA, but also support direct amplification of buccal swabs and blood with a final concentration of not more than 15%, eliminating the need for complicated extraction and preservation processes. The typing results are fast and accurate.
Pre-experiment Preparation and Important Notes
1. Before use, mix gently by turning up and down, avoid foaming as much as possible, and use after brief centrifugation. 2. Avoid repeated freezing and thawing of the product, which may degrade the performance of the product. This product can be stored at -20℃ for long term storage and protected from light. If frequent use is required within a short period of time, it can be stored at 2-8℃. procedure Take the primers for initial typing as an example: 1. PCR reaction system
2. Take the standards of different genotypes to be typed as templates and optimize the annealing temperatures respectively, so as to achieve better typing results. 3. Template processing. Blood templates can be directly diluted to different concentrations for amplification using ddH2O, and the recommended final concentration is 2% blood was used as template for typing amplification; for oral swab template, the swab could be used directly as template by gently scraping the inner wall of the oral cavity for about 6 times and placed in 400 μL-1000 μL of ddH2O after shaking and mixing. 4. PCR reaction program: This product can be used in a two-step PCR reaction program
Note: 1) Using the two-step PCR reaction program, if the signal is low or the CT value is large due to the use of primers with low Tm values, etc., try the three-step PCR amplification. 2）Real-time acquisition of signal curve method of typing and final acquisition of signal endpoint method of typing are both available. |
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