Name: 磁珠法病毒DNA/RNA提取试剂盒
SKU: M666148
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

产品内容

M666148	Component	96 T	Storage
M666148A	Buffer LB	60 mL	RT
M666148B	Buffer WB1	60 mL	RT
M666148C	Buffer WB2	60 mL	RT
M666148D	RNase-Free water	10 mL	RT
M666148E	Proteinase K	2×25 mg	RT
M666148F	Proteinase K Storage Buffer	2×1.25 mL	RT
M666148G	Magbeads PN	1.5 mL	RT

产品简介

本试剂盒适用于从全血、组织匀浆液、拭子，以及血清、血浆等无细胞体液中，简单、快速、高效地分离并纯化DNA/RNA。独特的缓冲体系使裂解液中的病毒核酸高效特异地结合在磁珠上，获得的病毒核酸纯度高，质量稳定，不含蛋白、核酸酶和其他杂质，可适用于各种常规操作，包括PCR、荧光定量PCR等实验。

自备仪器及试剂

1. 手动单管提取：

1）恒温混匀仪

2）2/15 mL磁力架

2. 与全自动核酸提取仪的匹配：

1）全自动核酸提取仪

2）96孔深孔板、 8联深孔磁套

3. 与全自动核酸提取仪的匹配：

1）全自动核酸提取仪

2）96孔深孔板， 96深孔板磁套

实验前准备及重要注意事项

1. 在实验前，应仔细阅读本说明书。

2. 第一次使用前向25mg Proteinase K中加入1.25mLProteinase K Storage Buffer使其溶解，室温保存。若需长期保存，请放置于-20℃。

3. 使用前请检查Buffer LB是否出现结晶或者沉淀，如有结晶或者沉淀，请将Buffer LB置于56℃ 水浴重新溶解。

操作步骤

1. 手动单管操作

1.1 样本裂解：

取1.5mL离心管（自备），加入20μL蛋白酶K（可选）， 200 μL样本（样本需平衡至室温）， 500 μLBuffer LB，涡旋震荡5秒后，置于80℃, 1200 rpm的恒温混匀仪上震荡混匀4min。

注：湿拭子样本，充分震荡混匀后取200 μL进行提取。干拭子样本浸泡于400μL生理盐水中，充分震荡混匀后静置5min，12000rpm离心1min后，取200 μL进行提取。

1.2 核酸吸附：

（1）向离心管中加入10μL Magbeads PN，涡旋震荡10s，置于室温， 1200rpm的恒温混匀仪上震荡混匀4min。

（2）将离心管置于磁力架，磁珠完全吸附后，小心吸弃所有液体。

1.3 样本漂洗1：

（1）向离心管中加入500μL Buffer WB1，涡旋震荡，置于室温， 1200rpm的恒温混匀仪上震荡混匀2min。

（2）将离心管置于磁力架，磁珠完全吸附后，小心吸弃所有液体。

1.4 样本漂洗2：

（1）向离心管中加入500μL Buffer WB2，涡旋震荡，置于室温， 1200rpm的恒温混匀仪上震荡混匀2min。

（2）将离心管置于磁力架，磁珠完全吸附后，小心吸弃所有液体。

1.5 核酸洗脱：

（1）晾干离心管2-5min，确保无乙醇残留。

（2）向离心管中加入100μL RNase-Free water，涡旋震荡， 56℃, 1200rpm的恒温混匀仪上震荡混匀5min。

（3）离心管置于磁力架上，磁珠吸附后，收集核酸溶液于新离心管中，置于-80℃长期保存。

2. 与仪器1的匹配

2.1 按照下表向96孔深孔板中加入相应试剂（样本需平衡至室温）：

2.2 将加样板放入仪器，按照下表编辑并运行提取程序：

位置	试剂及用量
1&7 列	Proteinase K : 20 μL 样本: 200 μL Buffer LB: 500μL
2&8 列	Buffer WB1: 500 μL
3&9 列	Buffer WB 2: 500 μL Magbeads PN: 10 μL
6&12 列	RNase-Free water :100 μL

编号	孔位	名称	等待时间（min）	混合时间（min）	磁吸时间（sec）	混合速度	体系 (μL）	温度 (℃)
1	3	收集	0	0	5	/	500	0
2	1	混合	0	4	0	快	700	80
3	1	混合	0	4	10	快	700	0
4	2	混合	0	1	5	快	500	0
5	3	混合	0	1	5	快	500	0
6	3	干燥	2	0	0	/	500	0
7	6	洗脱	0	4	10	快	100	56
8	2	释放	/	/	/	/	500	0

2.3 程序运行结束后，取出96深孔板，将第6、12列中的洗脱液转移至新离心管中， -80℃长期保存。

3. 与仪器2的匹配

3.1 按照下表向96孔深孔板中加入相应试剂（样本需平衡至室温）：

3.2 将加样板放入CWE9600仪器，按照下表编辑并运行提取程序：

位置	试剂及用量
磁套板	96深孔板磁套
样本板	Proteinase K : 20 μL 样本: 200 μL Buffer LB: 500μL
漂洗板1	Buffer WB1: 500 μL
漂洗板2	Buffer WB 2: 500 μL Magbeads PN: 10 μL
洗脱板	RNase-Free water :100 μL

盘位	温度1	温度2	温度3	4	5	温度6	7	8
体积μL	700	500	500	/	/	100	/	/
恒温温度	0	0	0	0	0	56	/	/
动作	正转	正转	正转	/	/	正转	/	/
名称	LB	WB1	WB2	/	/	EB	/	TIP

步序	盘位	温度	搅拌时间 min	搅拌速度 rpm	磁吸时间	风干时间 min	暂停
1	3	0	0	0	60	0	off
2	1	80	4	3000	0	0	off
3	1	0	4	3000	60	0	off
4	2	0	1	3000	60	0	off
5	3	0	1	3000	60	2	off
6	6	56	4	3000	60	0	off

3.3 程序运行结束后，取出96深孔板，将洗脱液转移至新离心管中， -80℃长期保存。

4. 与仪器3的匹配

4.1 按照下表向96孔深孔板中加入相应试剂（样本需平衡至室温）：

位置	试剂及用量
样本板	Proteinase K: 20 μL 样本: 200μL Buffer LB: 500 μL
漂洗板1	Buffer WB1: 500μL
漂洗板2	Buffer WB 2: 500μL Magbeads PN: 10 μL
洗脱板	无RNA酶的水:100μL

4.2 将加样板放入仪器3，按照下表编辑并运行提取程序：

编号	板位	名称	等待时间（min）	混合时间（min）	磁吸时间（sec）	混合速度	体系 (μL）	温度 (℃)
1	3	装磁套	/	/	/	/	/	/
2	3	收集	0	0	5	/	500	0
3	1	裂解	0	4	0	快	700	80
4	1	结合	0	4	10	快	700	0
5	2	漂洗	0	1	5	快	500	0
6	3	漂洗	0	1	5	快	500	0
7	3	干燥	2	0	0	/	500	0
8	6	洗脱	0	5	10	快	100	56
9	2	放磁套	/	/	/	/	/	/

4.3 程序运行结束后，取出96深孔板，将洗脱液转移至新离心管中， -80℃长期保存。

Product content

M666148	Component	96 T	Storage
M666148A	Buffer LB	60 mL	RT
M666148B	Buffer WB1	60 mL	RT
M666148C	Buffer WB2	60 mL	RT
M666148D	RNase-Free water	10 mL	RT
M666148E	Proteinase K	2×25 mg	RT
M666148F	Proteinase K Storage Buffer	2×1.25 mL	RT
M666148G	Magbeads PN	1.5 mL	RT

Product Introduction
This reagent kit is suitable for simple, rapid, and efficient separation and purification of DNA/RNA from whole blood, tissue homogenate, swabs, serum, plasma, and other cell-free body fluids. The unique buffer system enables the viral nucleic acid in the lysis solution to efficiently and specifically bind to magnetic beads, resulting in high purity and stable quality of viral nucleic acid. It does not contain proteins, nucleases, or other impurities and can be used for various routine operations, including PCR, fluorescence quantitative PCR, and other experiments.

Self provided instruments and reagents
1. Manual single tube extraction:
1) Constant temperature mixer
2) 2/15 mL magnetic rack
2. Matching with fully automatic nucleic acid extractor:
1) Fully automatic nucleic acid extractor
2) 96 hole deep hole plate, 8-pin deep hole magnetic sleeve
3. Matching with fully automatic nucleic acid extractor:
1) Fully automatic nucleic acid extractor
2) 96 hole deep hole plate, 96 deep hole plate magnetic sleeve
Preparation and important precautions before the experiment
1.Before the experiment, carefully read this manual.
2. Add 1.25mL Protein K Storage Buffer to 25mg Protein K before the first use to dissolve it, and store at room temperature. If long-term storage is required, please place it at -20 ℃.
3. Before use, please check if there is any crystallization or precipitation in the Buffer LB. If there is any crystallization or precipitation, please dissolve the Buffer LB again in a 56 ℃ water bath.

Operation steps
1. Manual single tube operation
1.1 Sample lysis:
Take 1.5mL centrifuge tube (self prepared) and add 20 μ L protease K (optional), 200 μ L sample (sample needs to be balanced to room temperature), 500 μ LBuffer LB, after vortex oscillation for 5 seconds, place it on a constant temperature mixer at 80 ℃ and 1200 rpm to shake and mix for 4 minutes.
Note: For wet swab samples, shake thoroughly and mix well before taking 200 samples μ L for extraction. Soak the dry swab sample in 400 μ In L physiological saline, thoroughly shake and mix, let stand for 5 minutes, centrifuge at 12000 rpm for 1 minute, and then take 200 μ L for extraction.
1.2 Nucleic acid adsorption:
(1) Add 10 to the centrifuge tube μ L Magheads PN, vortex oscillate for 10 seconds, place at room temperature, on a constant temperature mixer at 1200rpm, shake and mix for 4 minutes.
(2) Place the centrifuge tube on a magnetic frame, fully adsorb the magnetic beads, and carefully discard all liquids.
1.3 Sample rinsing 1:
(1) Add 500 to the centrifuge tube μ L Buffer WB1, vortex shake, place at room temperature, on a constant temperature mixer at 1200rpm, shake and mix for 2 minutes.
(2) Place the centrifuge tube on a magnetic frame, fully adsorb the magnetic beads, and carefully discard all liquids.
1.4 Sample rinsing 2:
(1) Add 500 to the centrifuge tube μ L Buffer WB2, vortex shake, place at room temperature, on a constant temperature mixer at 1200rpm, shake and mix for 2 minutes.
(2) Place the centrifuge tube on a magnetic frame, fully adsorb the magnetic beads, and carefully discard all liquids.
1.5 Nucleic acid elution:
(1) Dry the centrifuge tube for 2-5 minutes to ensure no ethanol residue.
(2) Add 100 to the centrifuge tube μ L RNase Free water, vortex oscillation, shake and mix on a constant temperature mixer at 56 ℃ and 1200rpm for 5 minutes.

(3) The centrifuge tube is placed on a magnetic frame, and after the magnetic beads are adsorbed, the nucleic acid solution is collected in a new centrifuge tube and stored at -80 ℃ for a long time.
2. Matching with instruments
2.1 Add the corresponding reagents to the 96 well deep well plate according to the table below (the sample needs to be balanced to room temperature):
2.2 Place the sample plate into the instrument, edit and run the extraction program according to the following table:

position	Reagent and dosage
1&7 列	Proteinase K : 20 μL sample: 200 μL Buffer LB: 500μL
2&8 列	Buffer WB1: 500 μL
3&9 列	Buffer WB 2: 500 μL Magbeads PN: 10 μL
6&12 列	RNase-Free water :100 μL

number	Hole position	name	waiting time （min）	mixing time （min）	Magnetic attraction time （sec）	mixing speed	system (μL）	temperature (℃)
1	3	collect	0	0	5	/	500	0
2	1	mix	0	4	0	fast	700	80
3	1	mix	0	4	10	fast	700	0
4	2	mix	0	1	5 fast	fast	500	0
5	3	mix	0	1	5	fast	500	0
6	3	dry	2	0	0	/	500	0
7	6	elution	0	4	10 fast	fast	100	56
8	2	release	/	/	/	/	500	0

2.3 After the program runs, remove the 96 well plate and transfer the eluent from columns 6 and 12 to a new centrifuge tube for long-term storage at -80 ℃.
3. Matching with Instrument 2
3.1 Add the corresponding reagents to the 96 well deep well plate according to the table below (the sample needs to be balanced to room temperature):
3.2 Place the sample plate into the CWE9600 instrument, edit and run the extraction program according to the following table:

position	Reagent and dosage
Magnetic sleeve plate	96 deep hole plate magnetic sleeve
Sample board	Proteinase K : 20 μL Sample : 200 μL Buffer LB: 500μL
Rinse plate 1	Buffer WB1: 500 μL
Rinse plate 2	Buffer WB 2: 500 μL Magbeads PN: 10 μL
Elution plate	RNase-Free water :100 μL

Drawer	temperature1	temperature2	temperature3	4	5	temperature6	7	8
volume μL	700	500	500	/	/	100	/	/
constant temperature	0	0	0	0	0	56	/	/
action	foreward	foreward	foreward	/	/	foreward	/	/
name	LB	WB1	WB2	/	/	EB	/	TIP

Step sequence	Drawer	temperature	mix time min	stirring speed rpm	Magnetic attraction time	Air drying time min	suspend
1	3	0	0	0	60	0	off
2	1	80	4	3000	0	0	off
3	1	0	4	3000	60	0	off
4	2	0	1	3000	60	0	off
5	3	0	1	3000	60	2	off
6	6	56	4	3000	60	0	off

After the program runs, remove the 96 well plate and transfer the eluent to a new centrifuge tube for long-term storage at -80 ℃.

4. Matching with Instrument 3
4.1 Add the corresponding reagents to the 96 well deep well plate according to the table below (the sample needs to be balanced to room temperature):

position	Reagent and dosage
Sample board	Proteinase K: 20 μL Sample : 200μL Buffer LB: 500 μL
Rinse plate 1	Buffer WB1: 500μL
Rinse plate 2	Buffer WB 2: 500μL Magbeads PN: 10 μL
Elution plate	Water without RNA enzyme:100μL

4.2 Place the sample plate into instrument 3, edit and run the extraction program according to the following table:

number	Plate position	name	waiting time （min）	mix time （min）	Magnetic attraction time （sec）	Mixing speed	system (μL）	temperature (℃)
1	3	Install magnetic sleeve	/	/	/	/	/	/
2	3	collect	0	0	5	/	500	0
3	1	splitting	0	4	0	Fast	700	80
4	1	combine	0	4	10	Fast	700	0
5	2	potch	0	1	5	Fast	500	0
6	3	potch	0	1	5	Fast	500	0
7	3	dry	2	0	0	/	500	0
8	6	elution	0	5	10	Fast	100	56
9	2	Magnetic discharge sleeve	/	/	/	/	/	/

After the program runs, remove the 96 well plate and transfer the eluent to a new centrifuge tube for long-term storage at -80 ℃.

规格

英文名称

Magbead Viral DNA/RNA Kit

储存与运输

储存条件

室温

运输条件

常规运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

m666148_magbead viral dnarna kit.pdf

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浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

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