Magbead Swab DNA Kit

Name: Magbead Swab DNA Kit
SKU: M667760
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器常见问题

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为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

产品内容

M667760	Component	96 T	Storage
M667760A	Buffer WL	36 mL	RT
M667760B	Buffer ML	36 mL	RT
M667760C	Buffer GW1 (concentrate)	80 mL	RT
M667760D	Buffer GW2 (concentrate)	50 mL	RT
M667760E	Buffer EB	30 mL	RT
M667760F	Proteinase K	2×1.25 mL	RT
M667760G	Magbeads PN	1 mL	RT

产品简介

该试剂盒提供了一种简单、快速、高效的口腔拭子DNA提取方法，适用于从干燥保存或在保护液中保存的口腔拭子中提取基因组DNA。在高盐存在时， DNA结合于硅基包被的Magbeads表面。漂洗后，高纯度的 DNA被洗脱于 Buffer EB或去离子水中。纯化得到的 DNA纯度好（A260/280的比值在1.7-1.9之间），完整度高（>15 kb），可用于二代测序、定量PCR、芯片检测等下游实验。

该试剂盒可与液体工作站和磁棒法磁珠自动提取系统匹配，简单、快速地进行高通量提取，大大降低了实验者的工作量和实验中的人为误差。

自备仪器、试剂

1. 手动单管提取：

1）恒温混匀仪

2）2/15 mL磁力架

3）异丙醇、无水乙醇

2. 手动96孔深孔板提取：

1）恒温混匀仪

2）废液抽吸系统

3）手动连续分液器

4）电动连续分液器

5）手动连续分液器分液管

6）96孔板磁力架

7）异丙醇、无水乙醇

实验前准备及重要注意事项

1. Magbeads严禁冰冻、离心。冰冻和离心可能会对Magbeads造成不可逆的损害。

2. Magbeads使用前需涡旋震荡20秒使其充分混匀。

3. 首次使用前应按试剂瓶标签在Buffer GW1和Buffer GW2中加入无水乙醇并做好标记。

4. 实验过程中，磁珠在溶液中的充分混匀对于提取的得率与纯度都有很大的影响。实验过程中务必使磁珠与溶液充分混匀。不同厂家生产的恒温混匀仪震荡混匀效果有一定差异，实验过程中请注意观察磁珠状态。如出现磁珠贴壁等未充分混匀的现象，请用移液器吹吸混匀或调整震荡频率。

操作步骤

一、手动单管操作

方案一（用于从干燥保存的口腔拭子中提取基因组DNA）：

1. 将口腔拭子的棉签用剪刀从杆上剪下，置于2.0 mL的离心管中。

2. 向离心管中加入20 μL Proteinase K和300 μL Buffer WL，之后将离心管放于56℃、 1600 rpm 的恒温混匀仪上震荡裂解30分钟。

注意：如无恒温混匀仪，将离心管涡旋震荡10秒钟后放于56℃水浴锅中孵育30分钟，期间每隔5分钟涡旋震荡10秒钟。

3. 将离心管从恒温混合仪上取下，短暂离心后加入300 μL Buffer ML。之后将离心管放于56℃、 1600 rpm的恒温混匀仪上震荡裂解10分钟。

注意：如无恒温混匀仪，将离心管涡旋震荡10秒钟后放于56℃水浴锅中孵育10分钟，期间每隔5分钟涡旋震荡10秒钟。

4. 将步骤3中的离心管短暂离心后室温放置5分钟，之后将裂解产物转移至1.5 mL的离心管中。向离心管中加入200 μL异丙醇和10 μL充分混匀的Magbeads，涡旋震荡5秒钟后将离心管放于 25℃、 1600 rpm的恒温混匀仪上震荡混匀5分钟或将离心管连续颠倒混匀10分钟。

5. 将离心管放于磁力架上静置1分钟，待Magbeads完全吸附于离心管侧壁后彻底弃去溶液（保持离心管固定于磁力架上）。

6. 将离心管从磁力架上取下，加入750 μL Buffer GW1（使用前请检查是否已加入无水乙醇）后涡旋点震1分钟或涡旋震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪上震荡混匀2分钟（震荡过程中确保Magbeads处于混匀状态）。之后将离心管放于磁力架上静置1分钟，待 Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离心管盖上的杂质洗落后彻底弃去溶液（保持离心管固定于磁力架上）。

7. 重复步骤6。

8. 将离心管从磁力架上取下，加入750 μL Buffer GW2（使用前请检查是否已加入无水乙醇）后涡旋点震1分钟或涡旋震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪上震荡混匀2分钟（震荡过程中确保Magbeads处于混匀状态）。之后将离心管放于磁力架上静置1分钟，待 Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离心管盖上的杂质洗落后彻底弃去溶液（保持离心管固定于磁力架上）。

9. 重复步骤8。

10.保持离心管固定于磁力架上，用移液器进一步去除离心管管底和管盖上的溶液，之后室温放置5-10分钟，使乙醇挥发干净。

注意：如果离心管侧壁上有液珠，可向离心管中加入750 μL无水乙醇。盖盖后颠倒离心管（保持离心管固定于磁力架上），之后彻底弃去无水乙醇。

11.将离心管从磁力架上取下，加入30-100 μL Buffer EB。涡旋震荡使磁珠完全悬浮于洗脱液中后将其放于56℃、 1600 rpm的恒温混匀仪上震荡洗脱10分钟，或将离心管放于56℃水浴锅中孵育10分钟，期间每隔3分钟涡旋震荡10秒钟。

12.将离心管放于磁力架上静置2分钟，待Magbeads完全吸附于离心管侧壁后用移液器将洗脱液转移至新的离心管中-20℃保存备用。

方案二（用于从在保护液中保存的口腔拭子中提取基因组DNA）：

1. 将口腔拭子的棉签从杆上剪下，置于2.0 mL的离心管中。

2. 向离心管中加入20 μL Proteinase K、300 μL保存该拭子的保护液和200 μL Buffer ML，之后将离心管放于56℃、 1600 rpm的恒温混合仪上震荡裂解30分钟。

注意：如无恒温混合仪，将离心管涡旋震荡10秒钟后放于56℃水浴锅中孵育30分钟，期间每隔5分钟涡旋震荡10秒钟。

3. 将离心管从从恒温混合仪上取下，室温放置5分钟。短暂离心后，将裂解产物转移至1.5 mL的离心管中。

4. 向离心管中加入300 μL异丙醇和10 μL充分混匀的Magbeads，涡旋震荡5秒钟后将离心管放于25℃、 1600 rpm的恒温混合仪上震荡混匀5分钟或将离心管连续颠倒混匀10分钟。

5. 将离心管放于磁力架上静置1分钟，待Magbeads完全吸附于离心管侧壁后充分弃去溶液（保持离心管固定于磁力架上）。

6. 将离心管从磁力架上取下，加入750 μL Buffer GW1 （使用前请检查是否已加入无水乙醇）后涡旋点震1分钟或涡旋震荡5秒钟后放于25℃、1600 rpm的恒温混匀仪上震荡混匀2分钟（震荡过程中确保Magbeads处于混匀状态）。之后将离心管放于磁力架上静置1分钟，待 Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离心管盖上的杂质洗落后彻底弃去溶液（保持离心管固定于磁力架上）。

7. 重复步骤6。

9. 重复步骤8。

10. 保持离心管固定于磁力架上，用移液器进一步去除离心管管底和管盖上的溶液，之后室温放置5-10分钟，使乙醇挥发干净。

注意：如果离心管侧壁上有液珠，可向离心管中加入750 μL无水乙醇。盖盖后颠倒离心管（保持离心管固定于磁力架上），之后彻底弃去无水乙醇。

12.将离心管放于磁力架上静置2分钟，待Magbeads完全吸附于离心管侧壁后用移液器将洗脱液转移至新的离心管中-20℃保存备用。

二、手动96孔板操作

方案三（用于在96孔深孔板中从干燥保存的口腔拭子中提取基因组DNA）

1.将口腔拭子棉签剪下放入96孔深孔板（简称“深孔板” ）中并记录每个孔中样品名称。

2.向加入口腔拭子的深孔板中用电动连续分液器或8通道移液器加入20 μL Proteinase K和300 μL Buffer WL，之后将深孔板放于100℃（该恒温混匀仪为悬空加热，裂解液实际温度在 50-60℃之间）、 1600 rpm的恒温混匀仪上震荡孵育30分钟。

3.将深孔板从恒温混匀仪上取下，加入300 μL Buffer ML。之后，将深孔板放于100℃（该恒温混匀仪为悬空加热，裂解液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡孵育 10分钟。

4.将深孔板从恒温混合仪上取下，把裂解产物按照顺序转移至新的深孔板中。

5.向新的深孔板中加入210 μL充分混匀的异丙醇（200 μL）与Magbeads（10 μL）混合物。之后将深孔板固定于25℃、 1600 rpm的恒温混合仪上震荡混匀5分钟。

6.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移液器弃去溶液，期间避免枪头接触磁珠。

注意：用废液抽吸系统去除溶液时需将真空泵调节至较小的负压力值，使溶液以一个合适的速度被吸走，速度过快

会造成磁珠的丢失。

7.用手动连续分液器向深孔板中加入500 μL Buffer GW1 （加入前检查是否已加入无水乙醇），之后将深孔板固定于25℃、 1600 rpm的恒温混匀仪上震荡混匀2分钟。

8.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移

液器弃去溶液，期间避免枪头接触磁珠。

9. 重复步骤7-8。

10.用手动连续分液器向深孔板中加入500 μL Buffer GW2（加入前检查是否已加入无水乙醇），之后将深孔板固定于25℃、 1600 rpm的恒温混匀仪上震荡混匀3分钟。

11.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移

液器弃去溶液，期间避免枪头接触磁珠。

12.重复步骤10-11。

13.用手动连续分液器向96孔深孔板中加入500 μL无水乙醇，之后将深孔板固定于25℃、 1600 rpm 的恒温混匀仪上震荡混匀1分钟。

14.将板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移液器弃去溶液，期间避免枪头接触磁珠。

15.保持深孔板固定于96孔板磁力架上，将深孔板倒置于干净的吸水纸上放置2分钟。之后将深孔板从96孔板磁力架上取下放于100℃、 1600 rpm的恒温混匀仪上静置5分钟。

16.用电动连续分液器或8通道移液器向深孔板加入50-100 μL Buffer EB，之后将深孔板放于 100℃（因该恒温混匀仪为悬空加热，洗脱液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡混匀10分钟。

17.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟，用8通道移液器溶液转移至 96孔PCR板中盖盖后-20℃保存备用。

方案四（用于在96孔深孔板中从在保护液中保存的口腔拭子中提取基因组DNA）：

1.将口腔拭子棉签剪下放入96孔深孔板（简称“深孔板”）中并记录每个孔中样品名称。

2.向加入口腔拭子的深孔板中用电动连续分液器或8通道移液器加入20 μL Proteinase K、300 μL 保护该拭子的保护液和200 μL Buffer ML，之后将深孔板放于100℃（该恒温混匀仪为悬空加

热，裂解液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡孵育30分钟。

3.将深孔板从恒温混匀仪上取下，把裂解产物按照顺序转移至新的深孔板中。

4.向新的深孔板中加入310 μL充分混匀的异丙醇（300 μL）与Magbeads（10 μL）混合物。之后将深孔板固定于25℃、 1600 rpm的恒温混合仪上震荡混匀5分钟。

5.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移液器弃去溶液，期间避免枪头接触磁珠。

注意：用废液抽吸系统去除溶液时需将真空泵调节至较小的负压力值，使溶液以一个合适的速度被吸走,速度过快会

造成磁珠的丢失。

6.用手动连续分液器向深孔板中加入500 μL Buffer GW1 （加入前检查是否已加入无水乙醇），之后将深孔板固定于25℃、 1600 rpm的恒温混匀仪上震荡混匀2分钟。

7.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移液器弃去溶液，期间避免枪头接触磁珠。

8. 重复步骤6-7。

9. 用手动连续分液器向深孔板中加入500 μL Buffer GW2（加入前检查是否已加入无水乙醇），之后将深孔板固定于25℃、 1600 rpm的恒温混匀仪上震荡混匀3分钟。

10.将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移液器弃去溶液，期间避免枪头接触磁珠。

11. 重复步骤9-10。

12. 用手动连续分液器向96孔深孔板中加入500 μL无水乙醇，之后将深孔板固定于25℃、 1600 rpm 的恒温混匀仪上震荡混匀1分钟。

13. 将板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟。用废液抽吸系统或8通道移液器弃去溶液，期间避免枪头接触磁珠。

14. 保持深孔板固定于96孔板磁力架上，将深孔板倒置于干净的吸水纸上放置2分钟。之后将深孔板从96孔板磁力架上取下放于100℃、 1600 rpm的恒温混匀仪上静置5分钟。

15. 用电动连续分液器或8通道移液器向深孔板加入50-100 μL Buffer EB，之后将深孔板放于 100℃（因该恒温混匀仪为悬空加热，洗脱液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡混匀10分钟。

16. 将深孔板从恒温混匀仪上取下后放于96孔板磁力架上静置2分钟，用8通道移液器溶液转移至 96孔PCR板中盖盖后-20℃保存备用。

三、与KingFisher Duo匹配

方案五（与KingFisher Duo匹配从干燥保存的口腔拭子中提取基因组DNA）：

1.将口腔拭子的棉签用剪刀从杆上剪下，置于2.0 mL的离心管中。

2.向离心管中加入20 μL Proteinase K和300 μL Buffer WL，之后将离心管放于56℃、 1600 rpm的恒温混匀仪上震荡裂解30分钟。

注意：如无恒温混匀仪，将离心管涡旋震荡10秒钟后放于56℃水浴锅中孵育30分钟，期间每隔5分钟涡旋震荡10秒钟。

3.将离心管从恒温混合仪上取下，短暂离心后加入300 μL Buffer ML。之后将离心管放于56℃、 1600 rpm的恒温混匀仪上震荡裂解10分钟。

注意：如无恒温混匀仪，将离心管涡旋震荡10秒钟后放于56℃水浴锅中孵育10分钟，期间每隔5分钟涡旋震荡10秒钟。

4.将离心管从恒温混匀仪上取下，短暂离心后把裂解产物转移至DW 96深孔板的A1-A12位置。 5. 按下表将试剂加入DW 96深孔板的相应位置：

名称	位置	试剂及用量
DW 96 深孔板	A1-A12	Lysate: 600 μL
DW 96 深孔板	B1-B12	KF Duo 12 道磁套
DW 96 深孔板	C1-C12	Buffer GW1: 750 μL
DW 96 深孔板	D1-D12	Buffer GW1: 750 μL
DW 96 深孔板	E1-E12	Buffer GW2: 750 μL
DW 96 深孔板	F1-F12	Buffer GW2: 750 μL
KF Duo 洗脱条	A1-A12	Buffer EB: 70 μL

方案六（与KingFisher Duo匹配从在保护液中保存的口腔拭子中提取基因组DNA）：
1. 将口腔拭子的棉签从杆上剪下，置于2.0 mL的离心管中。
2. 向离心管中加入20 μL Proteinase K、300 μL保存该拭子的保护液和200 μL Buffer ML，之后将离心管放于56℃、 1600 rpm的恒温混合仪上震荡裂解30分钟。
注意：如无恒温混合仪，将离心管涡旋震荡10秒钟后放于56℃水浴锅中孵育30分钟，期间每隔5分钟涡旋震荡10秒钟。
3. 将离心管从恒温混匀仪上取下，短暂离心后把裂解产物转移至DW 96深孔板的A1-A12位置。 4. 按下表将试剂加入DW 96深孔板的相应位置：

名称	位置	试剂及用量
DW 96 深孔板	A1-A12	Lysate: 500 μL
DW 96 深孔板	B1-B12	KF Duo 12 道磁套
DW 96 深孔板	C1-C12	Buffer GW1: 750 μL
DW 96 深孔板	D1-D12	Buffer GW1: 750 μL
DW 96 深孔板	E1-E12	Buffer GW2: 750 μL
DW 96 深孔板	F1-F12	Buffer GW2: 750 μL
KF Duo 洗脱条	A1-A12	Buffer EB: 70 μL

5. 向DW 96深孔板的A1-A12位置加入310 μL充分混匀的异丙醇（300 μL）与Magbeads（10 μL）混合物。

6. 打开KingFisher Duo仪器，运行CW1514_Duo程序。按照仪器提示将DW 96深孔板和KF Duo洗脱条放入仪器。

7. 约26分钟后程序运行结束，取出DW 96深孔板和KF Duo洗脱条，将洗脱条中的DNA溶液转移至

1.5 mL离心管中， -20℃保存。

四、与KingFisher Flex匹配

方案七（与KingFisher Flex匹配从干燥保存的口腔拭子中提取基因组DNA）：

1. 将口腔拭子棉签剪下放入96孔深孔板（简称“深孔板” ）中并记录每个孔中样品名称。 2 ．向加 2. 入口腔拭子的深孔板中用电动连续分液器或8通道移液器加入20μL Proteinase K

和 300 μL Buffer WL，之后将深孔板放于100℃（该恒温混匀仪为悬空加热，裂解液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡孵育30分钟。

3. 将深孔板从恒温混匀仪上取下，加入300 μL Buffer ML。之后，将深孔板放于100℃（该恒温混匀仪为悬空加热，裂解液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡孵育 10分钟。

4. 将深孔板从恒温混合仪上取下，把裂解产物按照顺序转移至“Sample Plate”中。

5. 按下表将试剂加入相应板中：

名称	类型	试剂及用量
Sample Plate	DW 96 深孔板	Lysate: 600 μL
Wash Plate I	DW 96 深孔板	Buffer GW1: 750 μL KF 96 DW 磁套
Wash Plate II	DW 96 深孔板	Buffer GW1: 750 μL
Wash Plate III	DW 96 深孔板	Buffer GW2: 750 μL
Wash Plate IV	DW 96 深孔板	Buffer GW2: 750 μL
Elution Plate	DW 96 深孔板	Buffer EB: 70 μL

6. 向“Sample Plate”中加入210 μL充分混匀的异丙醇（200 μL）与Magbeads（10 μL）混合物。

7. 打开KingFisher Flex仪器，运行CW2514_Flex程序。按照仪器提示将DW 96深孔板放入仪器中。

8. 约26分钟后程序运行结束，将DW 96深孔板取出。用封口膜将“Elution Plate”封闭， -20℃保存。

方案八（与KingFisher Flex匹配从在保护液中保存的口腔拭子中提取基因组DNA）：

1. 将口腔拭子棉签剪下放入96孔深孔板（简称“深孔板” ）中并记录每个孔中样品名称。

2. 向加入口腔拭子的深孔板中用电动连续分液器或8通道移液器加入20 μL Proteinase K、

300 μL保护该拭子的保护液和200 μL Buffer ML，之后将深孔板放于100℃（该恒温混匀仪为悬空加热，裂解液实际温度在50-60℃之间）、 1600 rpm的恒温混匀仪上震荡孵育30分钟。

3. 将深孔板从恒温混匀仪上取下，把裂解产物按照顺序转移至“Sample Plate”中。

4. 按下表将试剂加入相应板中：

名称	类型	试剂及用量
Sample Plate	DW 96 深孔板	Lysate: 500 μL
Wash Plate I	DW 96 深孔板	Buffer GW1: 750 μL KF 96 DW 磁套
Wash Plate II	DW 96 深孔板	Buffer GW1: 750 μL
Wash Plate III	DW 96 深孔板	Buffer GW2: 750 μL
Wash Plate IV	DW 96 深孔板	Buffer GW2: 750 μL
Elution Plate	DW 96 深孔板	Buffer EB: 70 μL

5. 向“Sample Plate”中加入310 μL充分混匀的异丙醇（300 μL）与Magbeads（10 μL）混合物。

6. 打开KingFisher Flex仪器，运行CW2514_Flex程序。按照仪器提示将DW 96深孔板放入仪器中。

7. 约26分钟后程序运行结束，将DW 96深孔板取出。用封口膜将“Elution Plate”封闭， -20℃ 保存。

Product content

M667760	Component	96 T	Storage
M667760A	Buffer WL	36 mL	RT
M667760B	Buffer ML	36 mL	RT
M667760C	Buffer GW1 (concentrate)	80 mL	RT
M667760D	Buffer GW2 (concentrate)	50 mL	RT
M667760E	Buffer EB	30 mL	RT
M667760F	Proteinase K	2×1.25 mL	RT
M667760G	Magbeads PN	1 mL	RT

Product Introduction
This kit provides a simple, fast, and efficient method for extracting genomic DNA from oral swabs that have been dried or preserved in protective solution. In the presence of high salt, DNA binds to the surface of silica coated Magheads. After rinsing, high-purity DNA is eluted in Buffer EB or deionized water. The purified DNA has good purity (A260/280 ratio between 1.7-1.9) and high integrity (>15 kb), and can be used for downstream experiments such as second-generation sequencing, quantitative PCR, and chip detection.
This reagent kit can be matched with liquid workstations and magnetic bead automatic extraction systems, allowing for simple and rapid high-throughput extraction, greatly reducing the workload of experimenters and human errors in experiments.

Self provided instruments and reagents
1. Manual single tube extraction:
1) Constant temperature mixer
2) 2/15 mL magnetic rack
3) Isopropanol and anhydrous ethanol
2. Manual 96 hole deep hole plate extraction:
1) Constant temperature mixer
2) Waste liquid suction system
3) Manual continuous liquid separator
4) Electric continuous liquid separator
5) Manual continuous liquid separator separation pipe
6) 96 hole plate magnetic bracket
7) Isopropanol and anhydrous ethanol

Preparation and important precautions before the experiment
1. Magheads are strictly prohibited from freezing or centrifugation. Freezing and centrifugation may cause irreversible damage to Magheads.
2. Before using Magheads, vortex and shake for 20 seconds to thoroughly mix.
3.Before first use, anhydrous ethanol should be added to Buffer GW1 and Buffer GW2 according to the label of the reagent bottle and labeled accordingly.
4.During the experiment, the thorough mixing of magnetic beads in the solution has a significant impact on the extraction yield and purity. During the experiment, it is essential to thoroughly mix the magnetic beads with the solution. There are certain differences in the shaking and mixing effects of constant temperature mixers produced by different manufacturers. Please pay attention to the state of the magnetic beads during the experiment. If there is insufficient mixing such as magnetic beads sticking to the wall, please use a pipette to blow and suck the mixture or adjust the oscillation frequency.
Operation steps
1、 Manual single tube operation
Option 1 (for extracting genomic DNA from dry preserved oral swabs):
1. Cut the cotton swab of the oral swab from the rod with scissors and place it in a 2.0 mL centrifuge tube.
2. Add 20 to the centrifuge tube μ L Protein K and 300 μ L Buffer WL, then place the centrifuge tube on a constant temperature mixer at 56 ℃ and 1600 rpm, shake and crack for 30 minutes.
Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 56 ℃ water bath for 30 minutes. During this period, vortex every 5 minutes for 10 seconds.
3. Remove the centrifuge tube from the constant temperature mixer, centrifuge briefly, and then add 300 μ L Buffer ML. Afterwards, place the centrifuge tube on a constant temperature mixer at 56 ℃ and 1600 rpm, shake and crack for 10 minutes.
Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 56 ℃ water bath for 10 minutes. During this period, vortex every 5 minutes for 10 seconds.
4. Centrifuge the centrifuge tube in step 3 briefly and let it stand at room temperature for 5 minutes. Then transfer the cracking product to a 1.5 mL centrifuge tube. Add 200 to the centrifuge tube μ L isopropanol and 10 μ Mix the Magheads thoroughly, vortex for 5 seconds, then place the centrifuge tube on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 5 minutes, or invert the centrifuge tube continuously for 10 minutes.

5. Place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
6. Remove the centrifuge tube from the magnetic frame and add 750 μ L Buffer GW1 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, then place it on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
7. Repeat step 6.
8. Remove the centrifuge tube from the magnetic frame and add 750 μ L Buffer GW2 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, then place it on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
9. Repeat step 8.
10. Keep the centrifuge tube fixed on the magnetic frame, use a pipette to further remove the solution from the bottom and cover of the centrifuge tube, and then leave it at room temperature for 5-10 minutes to allow the ethanol to evaporate completely.
Note: If there are liquid droplets on the sidewall of the centrifuge tube, 750 can be added to the centrifuge tube μ L anhydrous ethanol. After covering, invert the centrifuge tube (keeping it fixed on the magnetic frame), and then completely discard anhydrous ethanol.
11. Remove the centrifuge tube from the magnetic frame and add 30-100 μ L Buffer EB. Vortex oscillation causes the magnetic beads to completely suspend in the eluent and then place them on a constant temperature mixer at 56 ℃ and 1600 rpm for 10 minutes of shaking and elution, or incubate the centrifuge tube in a 56 ℃ water bath for 10 minutes, with vortex oscillation every 3 minutes for 10 seconds.
12. Place the centrifuge tube on a magnetic stand and let it stand for 2 minutes. After Magheads are completely adsorbed on the side wall of the centrifuge tube, use a pipette to transfer the eluent to a new centrifuge tube and store it at -20 ℃ for later use.

Option 2 (for extracting genomic DNA from oral swabs preserved in protective solution):
1. Cut the cotton swab of the oral swab from the rod and place it in a 2.0 mL centrifuge tube.
2. Add 20 to the centrifuge tube μ L Protein K, 300 μ Save the protective solution and 200 for the swab μ L Buffer ML, then place the centrifuge tube on a constant temperature mixer at 56 ℃ and 1600 rpm, shake and crack for 30 minutes.
Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 56 ℃ water bath for 30 minutes. During this period, vortex every 5 minutes for 10 seconds.
3. Remove the centrifuge tube from the constant temperature mixer and let it sit at room temperature for 5 minutes. After brief centrifugation, transfer the cracking product to a 1.5 mL centrifuge tube.
4. Add 300 to the centrifuge tube μ L isopropanol and 10 μ Mix the Magheads thoroughly, vortex for 5 seconds, and then place the centrifuge tube on a constant temperature mixer at 25 ℃ and 1600 rpm to shake and mix for 5 minutes or invert the centrifuge tube continuously for 10 minutes.
5. Place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, discard the solution thoroughly (keep the centrifuge tube fixed on the magnetic stand).
6. Remove the centrifuge tube from the magnetic frame and add 750 μ L Buffer GW1 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, then place it on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).

7. Repeat step 6.
8. Remove the centrifuge tube from the magnetic frame and add 750 μ L Buffer GW2 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, then place it on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
9. Repeat step 8.
10. Keep the centrifuge tube fixed on the magnetic frame, use a pipette to further remove the solution from the bottom and cover of the centrifuge tube, and then leave it at room temperature for 5-10 minutes to allow the ethanol to evaporate completely.
Note: If there are liquid droplets on the sidewall of the centrifuge tube, 750 can be added to the centrifuge tube μ L anhydrous ethanol. After covering, invert the centrifuge tube (keeping it fixed on the magnetic frame), and then completely discard anhydrous ethanol.
11. Remove the centrifuge tube from the magnetic frame and add 30-100 μ L Buffer EB. Vortex oscillation causes the magnetic beads to completely suspend in the eluent and then place them on a constant temperature mixer at 56 ℃ and 1600 rpm for 10 minutes of shaking and elution, or incubate the centrifuge tube in a 56 ℃ water bath for 10 minutes, with vortex oscillation every 3 minutes for 10 seconds.
12. Place the centrifuge tube on a magnetic stand and let it stand for 2 minutes. After Magheads are completely adsorbed on the side wall of the centrifuge tube, use a pipette to transfer the eluent to a new centrifuge tube and store it at -20 ℃ for later use.

2、 Manual 96 well plate operation
Option 3 (for extracting genomic DNA from dried oral swabs in a 96 well deep well plate)
1. Cut the oral swab cotton swab and place it into a 96 well deep well plate (referred to as the "deep well plate"), and record the name of the sample in each well.
2. Add 20 to the deep well plate where oral swabs are added using an electric continuous separator or 8-channel pipette μ L Protein K and 300 μ L Buffer WL, then place the deep well plate on a constant temperature mixer at 100 ℃ (the mixer is heated in the air, and the actual temperature of the cracking solution is between 50-60 ℃) and 1600 rpm for 30 minutes of shaking incubation.
3. Remove the deep hole plate from the constant temperature mixer and add 300 μ L Buffer ML. Afterwards, place the deep well plate on a constant temperature mixer at 100 ℃ (the constant temperature mixer is heated in the air, and the actual temperature of the cracking solution is between 50-60 ℃) and 1600 rpm, shake and incubate for 10 minutes.
4. Remove the deep well plate from the constant temperature mixer and transfer the cracking products in order to the new deep well plate.
5. Add 210 to the new deep hole plate μ Fully mixed isopropanol (200) μ L) And Magheads (10 μ L) Mix. Afterwards, fix the deep hole plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix well for 5 minutes.
6. Remove the deep well plate from the constant temperature mixer and place it on the 96 well plate magnetic stand for 2 minutes. Discard the solution using a waste liquid suction system or an 8-channel pipette, while avoiding contact between the gun tip and the magnetic beads.
Attention: When using the waste liquid suction system to remove the solution, the vacuum pump needs to be adjusted to a smaller negative pressure value to allow the solution to be sucked away at an appropriate speed, which is too fast will cause the loss of magnetic beads.
7. Use a manual continuous separator to add 500 to the deep orifice plate μ L Buffer GW1 (check if anhydrous ethanol has been added before adding), then fix the deep well plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes.
8. Remove the deep well plate from the constant temperature mixer and place it on the 96 well plate magnetic stand for 2 minutes. Use a waste liquid suction system or 8-channel transfer
Discard the solution from the liquid container and avoid the gun head coming into contact with the magnetic beads during the process.
9. Repeat steps 7-8.

10. Use a manual continuous liquid separator to add 500 to the deep orifice plate μ L Buffer GW2 (check if anhydrous ethanol has been added before adding), then fix the deep well plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 3 minutes.
11. Remove the deep well plate from the constant temperature mixer and place it on the 96 well plate magnetic stand for 2 minutes. Use a waste liquid suction system or 8-channel transfer
Discard the solution from the liquid container and avoid the gun head coming into contact with the magnetic beads during the process.
12. Repeat steps 10-11.
13. Use a manual continuous liquid separator to add 500 to the 96 well deep well plate μ L anhydrous ethanol, then fix the deep well plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 1 minute.
14. Remove the plate from the constant temperature mixer and place it on a 96 well plate magnetic stand for 2 minutes. Discard the solution using a waste liquid suction system or an 8-channel pipette, while avoiding contact between the gun tip and the magnetic beads.
15. Keep the deep hole plate fixed on the 96 hole plate magnetic bracket, invert the deep hole plate on clean absorbent paper and let it stand for 2 minutes. Afterwards, take the deep well plate from the 96 well plate magnetic holder and place it on a constant temperature mixer at 100 ℃ and 1600 rpm, and let it stand for 5 minutes.
16. Use an electric continuous separator or 8-channel pipette to add 50-100 to the deep orifice plate μ L Buffer EB, then place the deep well plate on a 100 ℃ (as the constant temperature mixer is heated in the air, the actual temperature of the eluent is between 50-60 ℃) and a 1600 rpm constant temperature mixer, shake and mix for 10 minutes.
17. Remove the deep well plate from the constant temperature mixer and place it on a 96 well plate magnetic stand for 2 minutes. Use an 8-channel pipette to transfer the solution to the 96 well PCR plate and cover it. Store at -20 ℃ for later use.

Option 4 (for extracting genomic DNA from oral swabs preserved in protective solution in a 96 well deep well plate):
1. Cut the oral swab cotton swab and place it into a 96 well deep well plate (referred to as the "deep well plate"), and record the name of the sample in each well.
2. Add 20 to the deep well plate where oral swabs are added using an electric continuous separator or 8-channel pipette μ L Protein K, 300 μ Protect the swab with protective solution and 200 μ L Buffer ML, then place the deep well plate at 100 ℃ (the constant temperature mixer is suspended and added)
3.Heat, the actual temperature of the cracking solution is between 50-60 ℃, and incubate with shaking on a constant temperature mixer at 1600 rpm for 30 minutes. 3. Remove the deep well plate from the constant temperature mixer and transfer the cracking products in order to the new deep well plate.
4. Add 310 to the new deep hole plate μ L Fully mixed isopropanol (300 μ L) And Magheads (10 μ L) Mix. Afterwards, fix the deep hole plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix well for 5 minutes.
5. Remove the deep well plate from the constant temperature mixer and place it on the 96 well plate magnetic stand for 2 minutes. Discard the solution using a waste liquid suction system or an 8-channel pipette, while avoiding contact between the gun tip and the magnetic beads.
Attention: When using the waste liquid suction system to remove the solution, the vacuum pump needs to be adjusted to a smaller negative pressure value to allow the solution to be sucked away at an appropriate speed. If the speed is too fast, it will causing the loss of magnetic beads.
6. Use a manual continuous separator to add 500 to the deep orifice plate μ L Buffer GW1 (check if anhydrous ethanol has been added before adding), then fix the deep well plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes.
7. Remove the deep well plate from the constant temperature mixer and place it on the 96 well plate magnetic stand for 2 minutes. Discard the solution using a waste liquid suction system or an 8-channel pipette, while avoiding contact between the gun tip and the magnetic beads.
8. Repeat steps 6-7.
9. Use a manual continuous liquid separator to add 500 to the deep orifice plate μ L Buffer GW2 (check if anhydrous ethanol has been added before adding), then fix the deep well plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 3 minutes.
10. Remove the deep well plate from the constant temperature mixer and place it on the 96 well plate magnetic stand for 2 minutes. Discard the solution using a waste liquid suction system or an 8-channel pipette, while avoiding contact between the gun tip and the magnetic beads.
11. Repeat steps 9-10.

12. Use a manual continuous liquid separator to add 500 to the 96 well deep well plate μ L anhydrous ethanol, then fix the deep well plate on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 1 minute.
13. Remove the plate from the constant temperature mixer and place it on a 96 well plate magnetic stand for 2 minutes. Discard the solution using a waste liquid suction system or an 8-channel pipette, while avoiding contact between the gun tip and the magnetic beads.
14. Keep the deep hole plate fixed on the 96 hole plate magnetic bracket, invert the deep hole plate on clean absorbent paper and let it stand for 2 minutes. Afterwards, take the deep well plate from the 96 well plate magnetic holder and place it on a constant temperature mixer at 100 ℃ and 1600 rpm, and let it stand for 5 minutes.
15. Use an electric continuous separator or 8-channel pipette to add 50-100 to the deep orifice plate μ L Buffer EB, then place the deep well plate on a 100 ℃ (as the constant temperature mixer is heated in the air, the actual temperature of the eluent is between 50-60 ℃) and a 1600 rpm constant temperature mixer, shake and mix for 10 minutes.
16. Remove the deep well plate from the constant temperature mixer and place it on a 96 well plate magnetic stand for 2 minutes. Use an 8-channel pipette to transfer the solution to the 96 well PCR plate and cover it. Store at -20 ℃ for later use.
3、 Match with KingFisher Duo
Option 5 (extracting genomic DNA from dry preserved oral swabs matched with KingFisher Duo):
1. Cut the cotton swab of the oral swab from the rod with scissors and place it in a 2.0 mL centrifuge tube.
2. Add 20 to the centrifuge tube μ L Protein K and 300 μ L Buffer WL, then place the centrifuge tube on a constant temperature mixer at 56 ℃ and 1600 rpm, shake and crack for 30 minutes.
Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 56 ℃ water bath for 30 minutes. During this period, vortex every 5 minutes for 10 seconds.
3. Remove the centrifuge tube from the constant temperature mixer, centrifuge briefly, and then add 300 μ L Buffer ML. Afterwards, place the centrifuge tube on a constant temperature mixer at 56 ℃ and 1600 rpm, shake and crack for 10 minutes.
Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 56 ℃ water bath for 10 minutes. During this period, vortex every 5 minutes for 10 seconds.
4. Remove the centrifuge tube from the constant temperature mixer, briefly centrifuge, and transfer the cracking product to the A1-A12 position on the DW 96 deep well plate. 5. Add the reagents to the corresponding positions of the DW 96 deep well plate according to the following table:

name	position	Reagent and dosage
DW 96 deep well plate	A1-A12	Lysate: 600 μL
DW 96 deep well plate	B1-B12	KF Duo 12 Magnetic sleeve
DW 96 deep well plate	C1-C12	Buffer GW1: 750 μL
DW 96 deep well plate	D1-D12	Buffer GW1: 750 μL
DW 96 deep well plate	E1-E12	Buffer GW2: 750 μL
DW 96 deep well plate	F1-F12	Buffer GW2: 750 μL
KF Duo Washout strip	A1-A12	Buffer EB: 70 μL

Option 6 (Extract genomic DNA from oral swabs preserved in protective solution, matched with KingFisher Duo):
1. Cut the cotton swab of the oral swab from the rod and place it in a 2.0 mL centrifuge tube.
2. Add 20 to the centrifuge tube μ L Protein K, 300 μ Save the protective solution and 200 for the swab μ L Buffer ML, then place the centrifuge tube on a constant temperature mixer at 56 ℃ and 1600 rpm, shake and crack for 30 minutes.

Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 56 ℃ water bath for 30 minutes. During this period, vortex every 5 minutes for 10 seconds.
3. Remove the centrifuge tube from the constant temperature mixer, briefly centrifuge, and transfer the cracking product to the A1-A12 position on the DW 96 deep well plate. 4. Add the reagents to the corresponding positions of the DW 96 deep well plate according to the following table:

name	position	Reagent and dosage
DW 96 deep well plate	A1-A12	Lysate: 500 μL
DW 96 deep well plate	B1-B12	KF Duo 12 Magnetic sleeve
DW 96 deep well plate	C1-C12	Buffer GW1: 750 μL
DW 96 deep well plate	D1-D12	Buffer GW1: 750 μL
DW 96 deep well plate	E1-E12	Buffer GW2: 750 μL
DW 96 deep well plate	F1-F12	Buffer GW2: 750 μL
KF Duo Washout strip	A1-A12	Buffer EB: 70 μL

5. Add 310 to the A1-A12 position of the DW 96 deep hole plate μ L Fully mixed isopropanol (300 μ L) And Magheads (10 μ L) Mix.
6. Open the KingFisher Duo instrument and run the CW1514-Duo program. Insert the DW 96 deep well plate and KF Duo elution strip into the instrument according to the instructions provided.
7.After about 26 minutes, the program runs and ends. Remove the DW 96 deep well plate and KF Duo elution strip, transfer the DNA solution from the elution strip to a 1.5 mL centrifuge tube, and store at -20 ℃.
4、 Matching with KingFisher Flex
Option 7 (Extract genomic DNA from dry preserved oral swabs matched with KingFisher Flex):
1. Cut the oral swab cotton swab and place it into a 96 well deep well plate (referred to as the "deep well plate"), and record the name of the sample in each well.
2. Add 2 to it Add 20 to the deep well plate of the oral swab using an electric continuous pipette or 8-channel pipette μ L Protein K and 300 μ L Buffer WL, then place the deep well plate on a constant temperature mixer at 100 ℃ (the mixer is heated in the air, and the actual temperature of the cracking solution is between 50-60 ℃) and 1600 rpm for 30 minutes of shaking incubation.
3. Remove the deep hole plate from the constant temperature mixer and add 300 μ L Buffer ML. Afterwards, place the deep well plate on a constant temperature mixer at 100 ℃ (the constant temperature mixer is heated in the air, and the actual temperature of the cracking solution is between 50-60 ℃) and 1600 rpm, shake and incubate for 10 minutes.
4. Remove the deep well plate from the constant temperature mixer and transfer the cracking products in order to the "Sample Plate".

5. Add the reagents to the corresponding plates according to the following table:

name	position	Reagent and dosage
Sample Plate	DW 96 deep well plate	Lysate: 600 μL
Wash Plate I	DW 96 deep well plate	Buffer GW1: 750 μL KF 96 DW Magnetic sleeve
Wash Plate II	DW 96 deep well plate	Buffer GW1: 750 μL
Wash Plate III	DW 96 deep well plate	Buffer GW2: 750 μL
Wash Plate IV	DW 96 deep well plate	Buffer GW2: 750 μL
Elution Plate	DW 96 deep well plate	Buffer EB: 70 μL

6. Add 210 to "Sample Plate" μ Fully mixed isopropanol (200) μ L) And Magheads (10 μ L) Mix.
7. Open the KingFisher Flex instrument and run the CW2514-Flex program. Insert the DW 96 deep well plate into the instrument according to the instrument prompts.
After about 26 minutes, the program ends and the DW 96 deep well plate is removed. Seal the "Elution Plate" with sealing film and store at -20 ℃.
Option 8 (Extract genomic DNA from oral swabs preserved in protective solution matched with KingFisher Flex):
1. Cut the oral swab cotton swab and place it into a 96 well deep well plate (referred to as the "deep well plate"), and record the name of the sample in each well.
2. Add 20 to the deep well plate where oral swabs are added using an electric continuous separator or 8-channel pipette μ L Protein K, 300 μ Protect the swab with protective solution and 200 μ L Buffer ML, then place the deep well plate on a constant temperature mixer at 100 ℃ (the mixer is heated in the air, and the actual temperature of the cracking solution is between 50-60 ℃) and 1600 rpm for 30 minutes of shaking incubation.
3. Remove the deep well plate from the constant temperature mixer and transfer the cracking products in order to the "Sample Plate".
4. Add the reagents to the corresponding plates according to the following table:

name	position	Reagent and dosage
Sample Plate	DW 96 deep well plate	Lysate: 500 μL
Wash Plate I	DW 96 deep well plate	Buffer GW1: 750 μL KF 96 DW Magnetic sleeve
Wash Plate II	DW 96 deep well plate	Buffer GW1: 750 μL
Wash Plate III	DW 96 deep well plate	Buffer GW2: 750 μL
Wash Plate IV	DW 96 deep well plate	Buffer GW2: 750 μL
Elution Plate	DW 96 deep well plate	Buffer EB: 70 μL

5. Add 310 to "Sample Plate" μ L Fully mixed isopropanol (300 μ L) And Magheads (10 μ L) Mix.
6. Open the KingFisher Flex instrument and run the CW2514-Flex program. Insert the DW 96 deep well plate into the instrument according to the instrument prompts.
7.After about 26 minutes, the program will finish running and the DW 96 deep well plate will be removed. Seal the "Elution Plate" with sealing film and store at -20 ℃.

规格

别名

Magbead 拭子 DNA 试剂盒

英文名称

Magbead Swab DNA Kit

储存与运输

储存条件

室温

运输条件

常规运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

m667760_magbead swab dna kit.pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

常见问题

本产品应如何储存？

请于室温条件下保存。

本产品如何运输？

本产品按常温标准条件运输，无需温控包装。

如何获取批次专用COA？

每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。

试剂盒包含哪些组分？

完整组分列表见PDP和试剂盒说明书。个别组分的替换品可单独订购——请联系您的客户代表。

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。