Name: 重组麦芽糖磷酸化酶 (MPL-EP)
SKU: M755092
Availability: InStock

重组麦芽糖磷酸化酶 (MPL-EP)

CAS: 9030-19-7 货号: M755092 EC号: 232-856-8

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,生物活性,重组级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

PROPERTIES

Molecular weight: ca. 220 kDa (gel filtration)

Structure: 2 subunits of 90 kDa (SDS-PAGE)

Michaelis constants: 1.9×10^−3 M (maltose); 3.4×10 ^ −3 M (phosphate); 8.3×10 ^ −3 M (arsenate)

pH Optimum: 6.5–7.5 (Fig. 1)

pH Stability: 5.5–8.0 (Fig. 2)

Optimum temperature: 45–50°C (Fig. 3)

Thermal stability: below 55°C (Fig. 4)

Stability (liquid form): stable at 37°C for at least one week (Fig. 5)

Stability (powder form): stable at 30°C for at least four weeks (Fig. 6)

Inhibitors: Hg ²⁺ , Ag ⁺ , Zn ²⁺ , Cu ²⁺

STABILIZER: lactose, EDTA

ASSAY PROCEDURE

Principle

The appearance of d-glucose is measured spectrophotometrically at 505 nm.

Reagents

A. HEPES–NaOH buffer, 50 mM; pH 7.0: dissolve 2.38 g of HEPES in 160 ml of distilled water, adjust to pH 7.0 with 1 N NaOH and dilute with distilled water to 200 ml.

B. Maltose solution, 0.2 M: 3.60 g of maltose monohydrate/50 ml of HEPES–NaOH buffer (Reagent A).

C. Phosphate solution, 0.2 M: dissolve 1.36 g of KH ₂ PO₄ in 40 ml of HEPES–NaOH buffer (Reagent A), adjust to pH 7.0 with 4 N NaOH and dilute with Reagent A to 50 ml.

D. HCl solution, 5 N: 43 ml of concentrated HCl/100 ml of distilled water.

E. pH Adjusting solution (NaOH solution, 1 n): 4.0 g of NaOH/100 ml of distilled water.

F. Glucose assay kit: “GLUCOSE C II-TESTWAKO” (Wako Pure Chemical), or a similar glucose assay kit.

Sample: dissolve the lyophilized enzyme to a volume activity of 0.15–0.55 U/ml with ice-cold HEPES–NaOH buffer (Reagent A) immediately before measurement.

Procedure

1. Pipette the following reagents into a test tube.

0.2 ml HEPES–NaOH buffer (Reagent A)

0.1 ml Maltose solution (Reagent B)

0.1 ml Phosphate solution (Reagent C)

2. Equilibrate at 30°C for about 5 min.

3. Add 0.1 ml of sample and incubate for 10 min at 30°C [test].The blank solution is prepared by adding HEPES–NaOH buffer (Reagent A) instead of sample [blank].

4. Add 0.1 ml of HCl solution (Reagent D) to stop the reaction.

5. Add 0.5 ml of pH adjusting solution (Reagent E).

6. Pipette 0.3 ml of the test and blank mixture into respective test tubes.

7. Add 3.0 ml of glucose assay kit (Reagent D) and incubate for about 5 min at 37°C.

8. Read the absorbance at 505 nm in a cuvette (light path: 1 cm) [test: AS, blank: A0].

Calculation

Activity can be calculated by using the following formula:

6.24: Millimolar extinction coefficient of quinoneimine dye under the assay conditions (cm ² /μmol)

1/10: A factor for correction of the reaction volume

df: Dilution factor

C: Content of maltose phosphorylase preparation in sample (mg/ml)

APPLICATIONS

The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

EXPERIMENTAL DATA

规格

产品名称

重组麦芽糖磷酸化酶 (MPL-EP)

英文别名

Maltose:orthophosphate 1-β-D-Glucosyltransferase | MALP

规格或纯度

生物活性, 重组, ActiBioPure™, EnzymoPure™, expressed in E.coli,≥10 U/mg powder

生化机理

Catalyzes the phosphorolysis of maltose, leading to the formation of glucose and glucose 1-P. Does not have maltose 6'-P phosphorylase activity. Plays an important role in the metabolism of maltose taken up via the bacterial phosphotransferase system (PTS).

生物活性

≥10 U/mg powder

表达系统

E. coli

来源

重组表达

预测分子量

220 kDa

CAS编号和信息

9030-19-7

酶学委员会编号

EC 2.4.1.8

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

expressed in E.coli,≥10 U/mg powder

复溶

Reconstitute in 50mM HEPES–NaOH buffer (pH 7.0) to a volume activity of 0.15-0.55 U/mL before use.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

长期储存-20℃（2年）；避免反复冻融。

单位定义

One unit (U) is defined as the amount of enzyme which produces 1 μmol of d-glucose per min at 30°C and pH 7.0 under the conditions described below.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到4个结果

批号(Lot Number)	证书类型	货号
ZJ26F0434469	分析证书	M755092
ZJ25F0926868	分析证书	M755092
ZJ25F0926867	分析证书	M755092
ZJ25F0926776	分析证书	M755092

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

需要帮助选择级别？

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查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 EnzymoPure™ 级别指南 →

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重组麦芽糖磷酸化酶 (MPL-EP)

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

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