α-来源于洋刀豆 ( 刀豆 ) 的甘露糖苷酶已用于:中性多糖的消化。从重组葡糖苷酶去除 O-聚糖抑制肾成纤维细胞增殖α-甘露糖苷酶可用于从各种合成和天然 α-甘露糖苷中释放甘露糖。它也被用于探究 mucolipidosis II ′ 敲入小鼠神经退化的原因。 从各种合成和天然 α-甘露糖苷中释放甘露糖。应用:
α−来源于洋刀豆的甘露糖苷酶是由两个亚基组成的三聚体,每个亚基含有 44 和 66kDa 的两个组成部分。 α-甘露糖苷酶是一种外糖苷酶和金属酶。溶酶体α-甘露糖苷酶是 II 类甘露糖苷酶,属于糖苷水解酶家族 38,在降解天冬酰胺链碳碳水化合物的糖蛋白中起重要作用。
α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.
α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.
Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.
Application:
α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.
α-Mannosidase from
Canavalia ensiformis (Jack bean) has been used:
- for the digestion of neutral glycans.
- to remove O-glycans from recombinant β-glucosidase
- for the inhibition of renal fibroblasts proliferation
Liberates mannose from a variety of synthetic and natural α-mannosides.