储存缓冲液：100% Isopropyl Alcohol
UltraBio™ NHS琼脂糖磁珠以交联琼脂糖为基质，拥有良好的刚性、磁响应性和单分散性，表面修饰有丰富的NHS（N-羟基琥珀酰亚胺）。通过NHS（N-羟基琥珀酰亚胺）活化基团与氨基反应进行蛋白质、多肽、糖等生物分子偶联是一项被广泛应用的成熟技术。含有伯胺基的配基可以直接与这个活化酯进行偶联，形成一个化学稳定性强的肽键。偶联条件温和、反应迅速、高稳定连接臂及键合方式等优点配合具有良好的刚性、磁响应性和单分散性的基球可以使您的配基充分发挥性能。
本产品每毫升磁珠悬液含约10%琼脂糖磁珠沉淀。
包装规格：10%（v/v）悬液
参数
| 指标
|
基质
| 琼脂糖
|
配基
| NHS（N-羟基琥珀酰亚胺）
|
含量
| Slurry: 10%
|
粒径
| 50 μm (20-80)
|
配基密度
| 30~50 µmol/mL (100%, v/v)
|
蛋白结合量
| 10~20 mg BSA/mL (100%, v/v)
|
保存
| 2-8℃ (100% 异丙醇） |
使用说明：
一、偶联准备
1、离心管、磁力架
2、反应缓冲液：0.1M PB, 0.15M NaCl, pH 8.3
3、清洗缓冲液：50mM Tris-HCl, 0.15M NaCl, pH 7.2
4、封闭液：0.1M Tris-HCl, pH 8.3
5、需要偶联的目标蛋白、抗体等生物配体。
二、操作流程
1、将磁珠混合均匀，取200μL 磁珠 (10%, V/V) 加入到2mL离心管中，磁性分离去除上清液。
注：“磁性分离”指将离心管置于外加磁场中，至磁珠吸附完全，约需要30s。
2、加入1 mL无水乙醇，混合均匀，磁性分离去除上清液（重复2次）。
3、加入500 µL反应缓冲液，混合重悬磁珠，磁性分离去除上清液（重复1次）。
4、加入200~400 µg抗体或者蛋白质溶液（提前用500 µL反应缓冲液溶解），室温旋转混合3h，磁性分离去除上清液。
5、加入1 mL封闭缓冲液，混合重悬磁珠，室温旋转混合2h，磁性分离去除上清液。
6、加入1 mL清洗缓冲液，混合重悬磁珠，磁性分离去除上清液（重复3~5次）。
7、将上述磁珠分散于0.5 mL PBS，pH 7.4短期保存，或分散于PBS，pH 7.4，0.02% NaN3长期保存。
注意事项：
1、偶联过程中不应含有除目标配体外含伯胺基团的物质，如：甘氨酸、BSA、Tris-HCl等，请注意您的样品溶液是否含有上述物质。
2、磁珠使用和保存过程中应避免冻融。
3、UltraBio™ NHS琼脂糖磁珠不可干燥。
4、不同抗体和蛋白与UltraBio™ NHS琼脂糖磁珠的结合能力不同，客户可自行优化加入不同 的抗体或者蛋白量。
5、此磁珠对湿度敏感，用完请及时密封，放置于2~8 ℃保存，使用前请将其恢复至室温后使用。
UltraBio™ NHS Magnetic Agarose Beads utilize cross-linked agarose as the matrix, offering excellent rigidity, magnetic responsiveness, and monodispersity. The beads are surface-modified with abundant NHS (N-Hydroxysuccinimide) groups. Protein, peptide, carbohydrate, and other biomolecule coupling via reaction between the NHS-activated group and amino groups is a mature and widely adopted technology. Ligands containing primary amine groups can directly couple with this activated ester, forming a chemically stable amide bond. The mild coupling conditions, rapid reaction kinetics, highly stable linker, and bonding mechanism—combined with the beads' exceptional rigidity, magnetic responsiveness, and monodispersity—enable optimal ligand performance.
Parameter
| Specification
|
Matrix
| Agarose
|
Ligand
| NHS
|
Content
| Slurry: 10%
|
Particle Size
| 50 μm (20-80)
|
Ligand Density
| 30~50 µmol/mL (100%, v/v)
|
Protein Binding Capacity
| 10~20 mg BSA/mL (100%, v/v)
|
Storage
| 2-8°C (in 100% Isopropanol) |
Instructions for Use:
I. Coupling Preparation
1.Equipment: Microcentrifuge tubes, Magnetic separation rack.
2.Reaction Buffer: 0.1 M PB, 0.15 M NaCl, pH 8.3
3.Wash Buffer: 50 mM Tris-HCl, 0.15 M NaCl, pH 7.2
4.Blocking Solution: 0.1 M Tris-HCl, pH 8.3
5.Target Ligand: Biological ligand for coupling (e.g., target protein, antibody).
II. Coupling Procedure
1.Resuspend the magnetic beads thoroughly. Transfer 200 µL of bead slurry (10%, V/V) to a 2 mL microcentrifuge tube. Perform magnetic separation and discard the supernatant.
Note: "Magnetic separation" refers to placing the tube in an external magnetic field until the beads are fully captured (approximately 30 seconds).
2.Add 1 mL anhydrous ethanol. Mix thoroughly. Perform magnetic separation and discard the supernatant. Repeat this ethanol wash step once more (2 washes total).
3.Add 500 µL Reaction Buffer. Resuspend the beads thoroughly. Perform magnetic separation and discard the supernatant.
4.Add 200-400 µg of antibody or protein solution (pre-dissolved in 500 µL Reaction Buffer). Incubate with end-over-end rotation at room temperature for 3 hours. Perform magnetic separation and discard the supernatant.
5.Add 1 mL Blocking Solution. Resuspend the beads thoroughly. Incubate with end-over-end rotation at room temperature for 2 hours. Perform magnetic separation and discard the supernatant.
6.Add 1 mL Wash Buffer. Resuspend the beads thoroughly. Perform magnetic separation and discard the supernatant. Repeat this wash step 3-5 times.
7.Resuspend the coupled beads in 0.5 mL PBS, pH 7.4 for short-term storage OR in PBS, pH 7.4, containing 0.02% NaN₃ for long-term storage.
Precautions:
1.Avoid Primary Amines: The coupling reaction should NOT contain substances with primary amine groups other than the target ligand (e.g., glycine, BSA, Tris-HCl). Please verify if your sample solution contains such interfering agents.
2.Avoid Freeze-Thaw: Beads should be protected from freeze-thaw cycles during use and storage.
3.Do Not Dry: UltraBio™ NHS Magnetic Agarose Beads must not be allowed to dry out.
4.Ligand Optimization: The binding capacity of UltraBio™ NHS Magnetic Agarose Beads varies for different antibodies and proteins. Users may need to optimize the amount of antibody or protein added.
5.Humidity Sensitivity & Storage: These beads are moisture-sensitive. Reseal the container immediately after use and store at 2-8°C. Allow the beads to equilibrate to room temperature before use.
ZJ26F0434480
危险品化学品经营许可证（带存储）