NF-κB-IN-14 (compound 5e) significantly inhibits nitric oxide production in LPS-induced macrophages ( IC 50 : 6.4 μM). NF-κB-IN-14 disrupts the TLR4-MyD88 protein interaction, leading to the suppression of the NF-κB signaling pathway suppression. NF-κB-IN-14 reduces ear edema and inflammation in an atopic dermatitis mouse model.