计算溶液所需的质量、体积或浓度。
for western blot,适用于电泳 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本产品由10种重组蛋白质(10~235 kDa)组成,条带锐利,分子量精准,分布合理,存放稳定。
使用说明
1、加样前在室温放置数分钟,解冻后,轻轻摇匀,以确保溶液混合均匀;
2、取用适量体积加样于凝胶孔,推荐用量如下:
厚度0.75~1.0 mm凝胶,上样5 μL/孔;厚度1.5 mm凝胶,上样10 μL/孔。也可根据情况酌情加样。
3、取用结束,盖上管盖,放回4℃或-20℃。
注:以上样5 μL为例,每个条带蛋白含量约为0.5 μg~1 μg。
注意事项
1、本产品可以加热煮沸;
2、在低浓度凝胶上,低分子量条带将会与前沿共迁移,而无法分出;
3、为避免反复冻融及污染,可将本产品分装后,-20℃保存;
4、本品含有多种化学成分,请勿吞食,避免和眼睛皮肤接触,为了您的安全和健康,请穿实验服并戴一次性手套操作;
5、本产品仅限科研使用。
This product consists of 10 recombinant proteins spanning a molecular weight range of 10–235 kDa. The marker provides sharp bands, accurate molecular weights, well-distributed size ranges, and excellent storage stability.
Protocol
Thaw the marker at room temperature for a few minutes before use. Gently mix to ensure homogeneity.
Load an appropriate volume into the gel wells:
Gel thickness 0.75–1.0 mm: Load 5 μL/well.
Gel thickness 1.5 mm: Load 10 μL/well.
Adjust the volume as needed based on experimental conditions.
After use, close the tube and store at 4°C or –20°C.
Note: For a 5 μL loading volume, each band contains approximately 0.5–1 μg of protein.
Precautions
This product can be heated/boiled (e.g., for denaturing gels).
In low-percentage gels, low-molecular-weight bands may co-migrate with the dye front and become unresolved.
To avoid repeated freeze-thaw cycles and contamination, aliquot and store at –20°C.
This product contains multiple chemical components. Do not ingest and avoid contact with eyes or skin. Wear a lab coat and disposable gloves during handling.
For research use only.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1491669 | |
| 分析证书 | N1491669 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Suitable for electrophoresis 级别指南 → 查看 for Western blot 级别指南 →