计算溶液所需的质量、体积或浓度。
NBT染色液（1mg/mL, pH7.8）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
植物组织在胁迫环境条件下会产生多种活性氧（ROS），ROS活性非常大且极其不稳定，因此ROS的检测通常因其最终产物而定。超氧阴离子是活性氧的一种，属于一种含氧自由基，能将NBT（氮蓝四唑）还原成不溶于水的蓝色甲瓒化合物，从而定位组织中的超氧阴离子。
NBT染色液（1mg/mL, pH7.8）由NBT和磷酸盐组成，用于植物活组织中的超氧阴离子染色。一般应用于较嫩的根尖、叶片等的整体染色，染色后有超氧阴离子聚集的部位呈蓝色至深蓝色。该产品仅用于科研领域，不用于临床诊断或其他用途。
| N1508545 | Component | 100 mL | Storage |
| N1508545A | NBT | 100 mg | 2-8℃. Store in the dark. |
| N1508545B | 磷酸缓冲液（pH7.8） | 100 mL | 2-8℃ |
自备仪器和试剂
- 新鲜的植物叶片或根、自来水、蒸馏水、95%乙醇
- 超声波、磁力搅拌器、电子天平、量筒、滤纸、照相机
- NBT染色样本保存液（可选）
操作步骤（仅供参考）
1、试剂准备：将100mg NBT加入到100mL磷酸缓冲液（pH7.8）中充分溶解，即得NBT染色工作液，4℃避光保存，一月内有效。
注：如果较难溶解，可通过超声、磁力搅拌等方法促溶。
2、样本准备：采集经胁迫（例如重金属）的采集植物幼苗或根尖，自来水稍洗净，置于滤纸上吸干多余水分。
3、染色：将植物幼苗或根尖浸入NBT染色工作液中，常温避光浸染2~6h，至阳性部位出现深蓝色，其余部位为淡蓝色或近无色或呈植物本身的颜色即可。（根据植物幼嫩程度，显色程度调整染色时间。）
4、脱色：用镊子将植物幼苗或者叶片小心取出，浸入蒸馏水中来回漂洗3~5次，置于滤纸上吸干多余水分后，浸入95%乙醇中40℃处理3~16h，目的是脱去植株幼苗或者叶片本身的叶绿素或者淡蓝色背景，处理期间可多次更换新鲜的95%乙醇。
5、观察：用镊子取出植株幼苗或者叶片，浸入蒸馏水中来回漂洗3~5次，置于滤纸上吸干多余水分后，拍照。（吸干水分后可将样本转入NBT染色样本保存液中浸泡30min，样本可置于该保存液中常温保存一周。）
注意事项
1、NBT染色工作液配制好以后需4℃避光保存，一月内使用。存放时间过久，会影响显色。
2、因任何外在因素都可能刺激植物应激产生超氧阴离子，因此植物样本需要新鲜采集，并尽快完成染色。建议做阴性及阳性空白对照组。
3、样本染色完成后尽快拍照保存结果。
4、染色和脱色步骤也可参考如下建议操作：组织放入染液中，抽真空，-0.1MPa保持负压20~30min，再于室温下静置染色60min，弃染色液；加入95%乙醇，于70~80℃水浴锅脱色，每隔10min换一次95%乙醇，待样品绿色全部褪去后可停止脱色。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Plant tissues produce various reactive oxygen species (ROS) under stress conditions. ROS are highly active and extremely unstable, so their detection is usually based on their end products. Superoxide anion is a type of ROS and belongs to a kind of oxygen-containing free radical. It can reduce NBT (nitroblue tetrazolium) into an insoluble blue formazan compound, thereby localizing superoxide anions in tissues.
NBT staining solution (1 mg/mL, pH 7.8) is composed of NBT and phosphate buffer, and is used for staining superoxide anions in living plant tissues. It is generally applied to whole staining of tender root tips, leaves, etc. After staining, areas with superoxide anion accumulation appear blue to dark blue. This product is for research use only and is not intended for clinical diagnosis or other purposes.
| N1508545 | Component | 100 mL | Storage |
| N1508545A | NBT | 100 mg | 2-8℃. Store in the dark. |
| N1508545B | Phosphate buffer (pH 7.8) | 100 mL | 2-8℃ |
Reagents, consumables and Equipments not provided
- Fresh plant leaves or roots, tap water, distilled water, 95% ethanol
- Ultrasonic cleaner, magnetic stirrer, electronic balance, measuring cylinder, filter paper, camera
- NBT staining sample storage solution (optional)
Procedure (For Reference Only)
1. Reagent preparation: Add 100 mg of NBT into 100 mL of phosphate buffer (pH 7.8) and dissolve thoroughly to obtain the NBT staining working solution. Store at 4°C protected from light. It is valid for one month.
Note: If dissolution is difficult, methods such as ultrasonication or magnetic stirring can be used to promote dissolution.
2. Sample preparation: Collect plant seedlings or root tips that have been subjected to stress (e.g., heavy metals). Rinse briefly with tap water and place on filter paper to blot excess moisture.
3. Staining: Immerse the plant seedlings or root tips into the NBT staining working solution. Incubate at room temperature protected from light for 2–6 hours, until positive areas appear dark blue, and the remaining areas are light blue, nearly colorless, or display the plant's original color. (Adjust staining time based on the tenderness and degree of coloration of the plant material.)
4. Decolorization: Carefully remove the plant seedlings or leaves with forceps, immerse them in distilled water and rinse by gently moving back and forth 3–5 times. Place on filter paper to blot excess moisture, then immerse in 95% ethanol and treat at 40°C for 3–16 hours. The purpose is to remove chlorophyll from the plant seedlings or leaves or to reduce the light blue background. During this treatment, replace with fresh 95% ethanol several times.
5. Observation: Remove the plant seedlings or leaves with forceps, immerse them in distilled water and rinse by gently moving back and forth 3–5 times. Place on filter paper to blot excess moisture, then photograph. (After blotting moisture, samples can be transferred into the NBT staining sample storage solution and soaked for 30 minutes. Samples can be stored in this solution at room temperature for up to one week.)
Notes
1. The prepared NBT staining working solution should be stored at 4°C protected from light and used within one month. Prolonged storage may affect coloration.
2. Since any external factors may stimulate plant stress and generate superoxide anions, plant samples should be freshly collected and stained as soon as possible. It is recommended to include negative and positive blank control groups.
3. Photograph and preserve the results promptly after sample staining is completed.
4. Staining and decolorization steps can also be performed with the following suggested modifications: Place the tissue in the staining solution, apply a vacuum, maintain at -0.1 MPa for 20–30 minutes, then allow to stain statically at room temperature for 60 minutes. Discard the staining solution. Add 95% ethanol and decolorize in a water bath at 70–80°C, replacing the 95% ethanol every 10 minutes. Stop decolorization when the green color of the sample has completely faded.
5. For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
别名
超氧阴离子染色液 | 氮蓝四唑染色液
英文别名
NBT Stain
规格或纯度
BioReagent,生物染色剂,用于显微镜,1 mg/mL
英文名称
NBT Staining Solution (1mg/mL, pH7.8)
应用
细胞染色
检测波长
0
敏感性
Light-sensitive
检测时长
0
样本体积
0
储存与运输
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
技术文档
高级数据
产品属性
| pH | 7.8 |
|---|
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0636282 |
分析证书 | N1508545 |
| ZJ26F0332379 |
分析证书 | N1508545 |
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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