计算溶液所需的质量、体积或浓度。
盐酸新品红溶液（4%）
有货
为什么选择此级别
BioReagent,生物染色剂 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
酸性α-乙酸萘酚酯酶（ANAE）染色又称非特异性酯酶染色，其原理是酸性条件下细胞中的酸性酯酶将α-乙酸萘酚水解产生α-萘酚，α-萘酚再与六偶氮副品红偶联，生成不溶性红色沉淀，定位于细胞质。
盐酸新品红溶液（4%）由盐酸和新品红等配制而成，与4%亚硝酸盐溶液等比例混合即产生六偶氮付品红（重氮盐），可用于酸性α-乙酸萘酚酯酶（ANAE）的染色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备仪器和试剂
- 显微镜、载玻片、湿盒
- 4%亚硝酸盐溶液等实验需要的其他配套试剂
操作步骤（仅供参考）
1、血液、骨髓或细胞涂片、冰冻切片用甲醛-丙酮缓冲液固定30~60s。水洗5min。
2、配制相关染色孵育液，根据操作流程进行操作。亦可参考酸性α-乙酸萘酚酯酶染色液（ANAE 法）进行操作。
染色结果
阳性部位呈暗红色或棕色。
注意事项
1、血液或骨髓细胞涂片应新鲜，薄厚适宜，一般2天内染色，否则会影响酶的活性。
2、盐酸副品红溶液含有盐酸，有一定的腐蚀性，应小心操作。
3、盐酸副品红溶液和亚硝酸盐溶液应临用前等比例混合，1min后再进行其他操作。二者混合时有刺激性气体产生。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Acid α-Naphthyl Acetate Esterase (ANAE) Staining, also known as non-specific esterase staining, is based on the principle that under acidic conditions, acid esterase in cells hydrolyzes α-naphthyl acetate to produce α-naphthol. The α-naphthol then couples with hexazotized pararosaniline to form an insoluble red precipitate, localized in the cytoplasm.
Neutral Red Hydrochloride Solution (4%) is prepared from hydrochloric acid and new fuchsin, among other components. When mixed in equal proportions with a 4% nitrite solution, it produces hexazotized pararosaniline (a diazonium salt), which can be used for Acid α-Naphthyl Acetate Esterase (ANAE) staining. This reagent is intended for scientific research only and is not suitable for clinical diagnosis or other purposes.
Reagents, consumables and Equipments not provided
- Microscope, glass slides, wet box,
- 4% nitrite solution, and other necessary supporting reagents for the experiment.
Operating Procedure (for reference only)
1. Fix blood, bone marrow, or cell smears, or frozen sections with formaldehyde-acetone buffer for 30–60 seconds. Rinse with water for 5 minutes.
2. Prepare the relevant staining incubation solution and proceed according to the operational workflow. Alternatively, refer to the Acid α-Naphthyl Acetate Esterase Staining Solution (ANAE method) for guidance.
Staining Results
Positive areas appear dark red or brown.
Precautions
1. Blood or bone marrow cell smears should be fresh and of appropriate thickness. Staining is generally recommended within 2 days to avoid affecting enzyme activity.
2. Hydrochloric acid pararosaniline solution contains hydrochloric acid and is somewhat corrosive. Handle with care.
3. Hydrochloric acid pararosaniline solution and nitrite solution should be mixed in equal proportions immediately before use. Wait for 1 minute before proceeding with further steps. Note that mixing these solutions produces irritating gases.
4. For safety and health, wear a lab coat and disposable gloves during operation.
5. After opening the reagent, use it as soon as possible to prevent affecting subsequent experimental results.
规格
别名
非特异性酯酶染色剂
英文别名
Neutral Red Hydrochloride Solution (4% w/v) | Neutral Red HCl Solution (4%)
规格或纯度
BioReagent, 生物染色剂, 4%
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
英文名称
Neutral Red Hydrochloride Solution (4%)
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332849 |
分析证书 | N1510329 |
技术文档和文章
溶液计算器
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