BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
铁是人体必需且含量最高的微量元素，通常以三价铁形式与转铁蛋白结合进入细胞，经还原为二价铁后参与多项生理生化过程。铁缺乏会导致贫血、免疫力下降及神经、消化系统功能异常，但铁过量同样有害。细胞内过量的二价铁可形成不稳定铁池，通过芬顿反应产生羟基自由基等活性氧，引发脂质过氧化，导致细胞铁死亡——这是铁稳态失衡的重要病理机制之一。 该试剂盒通过特定方法排除血红素铁干扰，精准反映组织或细胞内贮存铁的变化。其原理是样本经酸热处理并离心后，非血红素铁释放至上清液，经还原剂作用转化为二价铁，与红菲咯啉形成在500–550 nm有强吸收的红色化合物，可用分光光度法或酶标仪测定。本产品适用于动物组织或细胞提取物中非血红素铁的科研检测，不用于临床诊断。
Iron is an essential trace element and the most abundant micronutrient in the human body. It is typically transported into cells as ferric iron bound to transferrin, where it is reduced to ferrous iron and participates in various physiological and biochemical processes. Iron deficiency can lead to anemia, weakened immunity, and impairments in nervous and digestive system functions, while iron overload is equally harmful. Excess ferrous iron within cells can form an unstable iron pool, which generates hydroxyl radicals and other reactive oxygen species (ROS) via the Fenton reaction. This triggers lipid peroxidation, leading to ferroptosis—a key pathological mechanism resulting from disrupted iron homeostasis. This kit employs a specific method to exclude interference from heme iron, allowing accurate detection of stored iron levels in tissues or cells. The principle involves acid-heat treatment and centrifugation of the sample to release non-heme iron into the supernatant. The iron is then reduced to ferrous iron, which forms a red-colored complex with bathophenanthroline, exhibiting strong absorption between 500–550 nm. The absorbance can be measured using a spectrophotometer or microplate reader. This product is intended for the research detection of non-heme iron in animal tissues or cell extracts and is not designed for clinical diagnosis.
危险品化学品经营许可证（带存储）