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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖转化酶(Invertase,Ivr)催化蔗糖不可逆地分解为果糖和葡萄糖,是高等植物蔗糖代谢关键酶之一。根据最适 pH,将高等植物Ivr分为酸性转化酶(AI)和中性转化酶(NI)两种类型。NI主要存在于细胞质中,负责分解细胞质中蔗糖为果糖和葡萄糖。 测定原理:NI催化蔗糖分解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,在510nm有特征光吸收,在一定范围内510nm光吸收值与还原糖生成量成正比。通过光吸收增加速率来计算NI活性。
Sucrose invertase (Ivr) catalyzes the irreversible decomposition of sucrose into fructose and glucose and is one of the key enzymes in sucrose metabolism in higher plants. Based on optimal pH, Ivr in higher plants is divided into two types: acid invertase (AI) and neutral invertase (NI). NI is mainly present in the cytoplasm and is responsible for the breakdown of cytoplasmic sucrose into fructose and glucose. Determination principle: NI catalyzes the decomposition of sucrose to produce reducing sugars, which further react with 3,5‑dinitrosalicylic acid to form a reddish‑brown amino compound with characteristic light absorption at 510 nm. Within a certain range, the absorbance at 510 nm is proportional to the amount of reducing sugars produced. NI activity is calculated by measuring the rate of increase in absorbance.