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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
5'-核苷酸酶能够催化核苷-5'-磷酸水解生成核苷和无机磷，无机磷在酸性条件下能够与钼酸铵反应生成磷钼酸铵，经还原后生成蓝色磷钼蓝，产物在660 nm处具有特征吸收峰，通过吸光值变化即可表征5'-核苷酸酶的活性。
5'-Nucleotidase (5'-NT) catalyzes the hydrolysis of nucleoside-5'-monophosphate to nucleoside and inorganic phosphate (Pi). Under acidic conditions, the released inorganic phosphate reacts with ammonium molybdate to form ammonium phosphomolybdate, which is subsequently reduced to blue molybdenum blue. The resulting product exhibits a characteristic absorbance maximum at 660 nm. The activity of 5'-nucleotidase can be quantified by monitoring the change in absorbance at this wavelength.
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