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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酸合成酶（GOGAT）广泛分布于植物中，是氮素同化过程的关键酶。植物吸收的无机氮经硝酸还原酶（NR）和亚硝酸还原酶（NiR）还原为NH₄⁺后，通过谷氨酰胺合成酶（GS）与GOGAT协同参与的GS/GOGAT途径完成氮素的同化与利用。GOGAT一般分为两类：一类是多存在于叶绿体（叶片）中的Fd-GOGAT（EC 1.4.7.1），另一类是多存在于非绿色组织（根）前质体中的NADH-GOGAT（EC 1.4.1.14）。本试剂盒测定的是NADH-GOGAT的酶活性，其催化谷氨酰胺的氨基转移至α-酮戊二酸，生成两分子谷氨酸，同时将NADH氧化为NAD⁺。通过监测340 nm处NADH特征吸收峰的下降速率，在底物饱和条件下，单位时间内OD₃₄₀的初始线性降幅（ΔOD₃₄₀/min）与NADH-GOGAT酶活性呈正比，据此即可精准计算样品中的酶活力大小。 反应方程式： L-谷氨酰胺 + α-酮戊二酸 + NADH + H⁺ ⟶ 2 L-谷氨酸 + NAD⁺
Glutamate synthase (GOGAT) is widely distributed in plants and is a key enzyme in the nitrogen assimilation process. After inorganic nitrogen absorbed by plants is reduced to NH₄⁺ via nitrate reductase (NR) and nitrite reductase (NiR), it is assimilated and utilized through the GS/GOGAT pathway involving glutamine synthetase (GS) and GOGAT in a coordinated manner. GOGAT is generally classified into two types: Fd-GOGAT (EC 1.4.7.1), which is predominantly found in chloroplasts (leaves), and NADH-GOGAT (EC 1.4.1.14), which is mainly present in proplastids of non-green tissues (roots). This kit measures the activity of NADH-GOGAT, which catalyzes the transfer of the amino group from glutamine to α-ketoglutarate, generating two molecules of glutamate while oxidizing NADH to NAD⁺. By monitoring the rate of decrease in the characteristic absorbance peak of NADH at 340 nm, under substrate-saturated conditions, the initial linear decrease in OD₃₄₀ per unit time (ΔOD₃₄₀/min) is proportional to NADH-GOGAT activity. Accordingly, the enzyme activity in the sample can be precisely calculated. Reaction equation: L-Glutamine + α-Ketoglutarate + NADH + H⁺ ⟶ 2 L-Glutamate + NAD⁺
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