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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
山梨醇脱氢酶（SDH）是调控生物体内山梨醇代谢的关键酶，依据辅酶依赖性分为NADP⁺依赖型（NADP⁺-SDH，EC 1.1.1.B60）和NAD⁺依赖型（NAD⁺-SDH）。本试剂盒特异性测定NADP⁺-SDH活力。 检测原理：NADP⁺-SDH催化山梨醇脱氢生成葡萄糖，同时将NADP⁺还原为NADPH。NADPH在340 nm处具有特征吸收峰，通过连续监测该波长下吸光度的增加速率（其与NADPH生成量及NADP⁺-SDH活力成正比），即可定量计算样品中NADP⁺-SDH的酶活力。
Sorbitol dehydrogenase (SDH) is a key enzyme regulating sorbitol metabolism in organisms, and is classified into NADP⁺-dependent (NADP⁺-SDH, EC 1.1.1.B60) and NAD⁺-dependent (NAD⁺-SDH) types based on coenzyme specificity. This kit specifically measures the activity of NADP⁺-SDH. Principle of the assay: NADP⁺-SDH catalyzes the dehydrogenation of sorbitol to glucose, while simultaneously reducing NADP⁺ to NADPH. NADPH exhibits a characteristic absorbance peak at 340 nm. By continuously monitoring the rate of absorbance increase at this wavelength—which is proportional to the amount of NADPH generated and thus to NADP⁺-SDH activity—the enzyme activity in the sample can be quantitatively calculated.
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