BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酸脱氢酶（GDH）广泛分布于生物体中，在氨同化及有机氮化合物代谢中发挥重要作用。该酶以NADPH或NADH为辅酶，动植物中两种辅酶形式均有存在，但酵母中主要为NADPH依赖性谷氨酸脱氢酶（NADPH-GDH，EC 1.4.1.4）。本试剂盒提供一种快速、灵敏的检测方法：NADPH-GDH特异性催化谷氨酸脱氢生成α-酮戊二酸和NADPH（逆向反应），生成的NADPH与特异显色剂反应，产生在450 nm处具有最大吸收峰的黄色产物。通过动力学模式连续监测单位时间内OD₄₅₀的初始线性增幅（ΔOD₄₅₀/min），即可精准计算样品中NADPH-GDH的酶活力大小。 反应方程式： L-谷氨酸 + NADP⁺ + H₂O ⟶ α-酮戊二酸 + NH₄⁺ + NADPH + H⁺
Glutamate dehydrogenase (GDH) is widely distributed in organisms and plays an important role in ammonia assimilation and the metabolism of organic nitrogen compounds. This enzyme utilizes NADPH or NADH as coenzymes; both coenzyme forms exist in animals and plants, but in yeast, the NADPH-dependent glutamate dehydrogenase (NADPH-GDH, EC 1.4.1.4) is predominant. This kit provides a rapid and sensitive assay: NADPH-GDH specifically catalyzes the oxidative deamination of glutamate to α-ketoglutarate and NADPH (reverse reaction). The generated NADPH reacts with a specific chromogenic reagent to produce a yellow product with a maximum absorbance peak at 450 nm. By continuously monitoring the initial linear increase in OD₄₅₀ per unit time (ΔOD₄₅₀/min) in kinetic mode, the NADPH-GDH enzyme activity in the sample can be precisely calculated. Reaction equation: L-Glutamate + NADP⁺ + H₂O ⟶ α-Ketoglutarate + NH₄⁺ + NADPH + H⁺
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