计算溶液所需的质量、体积或浓度。
用于细胞培养 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
营养混合物Ham#8242;s F-12由Ham开发,其含有较高水平的氨基酸、维生素和微量元素。其将腐胺和亚油酸加入配方中。该营养培养基最初设计用于中国仓鼠卵巢和肺细胞的无血清生长。当与全血或透析血清一起使用或与激素和转铁蛋白组合使用时,它可广泛用于哺乳动物和杂交瘤细胞的生长。 配方中每升培养基含有11.1克的粉末。
应用
营养混合物F-12 Ham可用于以下应用:HeLa(人子宫颈腺癌细胞)细胞在补充有10%热灭活胎牛血清的该培养基(营养混合物F-12 Ham)进行中生长在维持和培养期间用于不同细胞的细胞培养(在营养混合物F-12 HAM培养基与其他组分中,A549细胞系保持为单层)用作Cowdria ruminantium繁殖的培养基用于制备初级混合胶质细胞培养物Kaighn#8242;s 改良的Ham#8242;s F-12和Coon#8242;s F-12增加了氨基酸和丙酮酸,以及改性盐(Konigsbergs)的浓度。该培养基被设计用于支持分化的大鼠和鸡细胞以及原代人肝细胞的生长。
适用性
原理
制备说明
外形
重悬
并补充2.5 g/L碳酸氢钠。
组分
The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.Nutrient Mixture F-12 Ham is used for the following applications:HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serumUsed in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)Used in media for the propagation ofCowdria ruminantiumUsed during preparation of primary mixed glial culturesKaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.Quantity: Formulated to contain 11.1 grams of powder per liter of medium.Reconstitution: Supplement with 2.5 g/L sodium bicarbonate.