新霉素溶液

货号: N485930
有货
级别和纯度: ~10 mg/mL in 0.9% NaCl,经0.1μm滤膜过滤,生物试剂,适用于细胞培养
储存条件
2-8°C储存
运输条件
低温运输
★
规格
库存
价格
数量
20ml
N485930-20ml
期货 Stock Image
¥269.90
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概述

化学结构:氨基糖苷类
应用:
新霉素溶液可用于:
作为骨髓移植用高压灭菌酸化水的补充剂
筛选稳定的克隆
作为人工海水(ASWO)培养基的组分,以从 O. tauri 培养物中去除细菌
免责声明
将该溶液配制为含有10 mg/mL新霉素的0.9%氯化钠溶液。在37 °C下可稳定保存5天,否则应在2-8°C下保存。
Chemical structure: aminoglycoside
Application
Neomycin solution has been used:
as a supplement of autoclaved acidified water for bone marrow transplantation
for the selection of stable clones
as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures
Biochem/physiol Actions
Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria
Disclaimer
This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

规格

规格或纯度
~10 mg/mL in 0.9% NaCl,经0.1μm滤膜过滤,生物试剂,适用于细胞培养
英文名称
Neomycin solution
生化机理
新霉素是一种氨基糖苷类抗生素,由链霉菌产生,含有至少85%的新霉素B。它可用作新选择标记基因转化的原核细胞的筛选剂。三硫酸新霉素可用于研究抗生素的细胞毒性副作用、某些成纤维细胞血小板衍生生长因子的应答以及提取核磷脂酰肌醇4,5-二磷酸互作蛋白。该产品的推荐细胞培养使用浓度为5 mL/L。作用机制:该产品通过和30S以及50S亚基结合,引起错编,抑制蛋白质合成过程的起始和延长。 新霉素也可阻断电压敏感性Ca2+通道,而不会影响神经元中的Na+/Ca2+逆向转运蛋白。抗菌谱:新霉素能够作用于革兰氏阳性和阴性细菌。
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
未知

高级数据

化学和物理性质
敏感性
将该溶液配制为含有10 mg/mL新霉素的0.9%氯化钠溶液。在37 °C下可稳定保存5天,否则应在2-8°C下保存。
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批号(Lot Number) 证书类型 货号
D2610549D2610549 分析证书 N485930
溶液计算器