化学结构:氨基糖苷类
应用:
新霉素溶液可用于:
作为骨髓移植用高压灭菌酸化水的补充剂
筛选稳定的克隆
作为人工海水(ASWO)培养基的组分,以从 O. tauri 培养物中去除细菌
免责声明
将该溶液配制为含有10 mg/mL新霉素的0.9%氯化钠溶液。在37 °C下可稳定保存5天,否则应在2-8°C下保存。
Chemical structure: aminoglycoside
Application
Neomycin solution has been used:
as a supplement of autoclaved acidified water for bone marrow transplantation
for the selection of stable clones
as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures
Biochem/physiol Actions
Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria
Disclaimer
This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.