计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product Description
N-acetylglucosaminidase cleaves all non-reducing terminal β-linked N-acetylglucosamine residues from complex carbohydrates and glycoproteins.
The cleavage rates of different linkages of GlcNAc on bi-, tri- and tetraantennary oligosaccharides is greatly dependent on the steric hindrance by neighboring residues. The β(1-2)GlcNAc residue linked to the β(1-3)-linked mannose is cleaved at the highest rate and the β(1-2) GlcNAc residue linked to the β(1-6)-linked mannose at the lowest rate for all three oligosaccharides. The β(1-6) GlcNAc residue, when present, is removed at the second highest rate and the β(1-4) GlcNAc, third. On a triantennary structure, this residue is removed at the second highest rate. A bisecting β(1-4) GlcNAc linked to the β-linked mannose severely hinders cleavage of other GlcNAc residues–high concentrations of enzymes and prolonged incubation times are required for cleavage.
Contents
N-Acetylglucosaminidase in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
included with 20 µL and 60 µL pack sizes:
1 vial: 5x Reaction buffer
250mM Sodium phosphate, pH 5
Molecular Weight
~140,000 daltons
pH optimum
5.0, active over the range 5-7
Specificity
All non-reducing terminal ß-linked N-acetylglucosamine. Bisecting GlcNAc slows the reaction.
Specific Activity Assay
One unit of N-acetylglucosaminidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37°C, pH 5.0 from p-nitrophenyl-ß-D-N-acetyl-glucosaminide.
Formulation
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Purity
N-acetylglucosaminidase is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases.
Directions for use
1. Add up to 100 µg of asialogalacto-glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add de-ionized water to a total of 14 µl.
3. Add 4 µl 5x Reaction Buffer 5.0.
4. Add 2 µl N-acetylglucosaminidase
5. Incubate at 37°C for 3 hours. If bisecting GlcNAc is present, incubation time should be increased to 12 hours.
Progress may be monitored by SDS-PAGE if the size differential between native and de-glycosylated protein is sufficient for detection.
Application
•Structural analysis of oligosaccharides
•Distinguishing different N-acetyl glucosamine link-ages
•Distinguishing between N-acetyl glucosamine and N-acetylgalactosamine
•Removing heterogeneity from glycoproteins
规格
产品名称
N-Acetylglucosaminidase
别名
N-乙酰葡萄糖胺酶
英文别名
ß-N-acetylglucosaminidase | glucosaminidase | N-acetyl-β-d-glycosaminide | hexosaminidase | N-acetylglucosaminohydrolase
规格或纯度
Specific Activity＞80 U/mg;Activity＞50 U/ml
生物活性
>50 U/ml
酶学委员会编号
3.2.1.52
分子类型
肽
产品线
生物产品
储存与运输
浓度
Specific Activity＞80 U/mg;Activity＞50 U/ml
储存条件
2-8°C储存
运输条件
低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
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产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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