计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
NADPH oxidase-IN-1 is an orally active NADPH oxidase ( Nox ) inhibitor, related with neuronal inflammation. NADPH oxidase-IN-1 can cross the blood-brain barrier (BBB), inhibits Nox2 and Nox4 with IC 50 s of 1.9 μM and 2.47 μM, respectively. NADPH oxidase-IN-1 suppresses pro-inflammatory cytokines production and LPS-mediated microglial migration, also has in vivo efficacy
In Vitro
NADPH oxidase-IN-1 (compound 11) can cross cross the blood-brain barrier (BBB) with P e value of 13.6 (10 -6 cm/s), determined by parallel artificial membrane permeability assay (PAMPA assay). NADPH oxidase-IN-1 (1 nM-10 mM; 30 min) LPS-induced ROS generation in BV2 microglial cells in a dose-dependent manner. NADPH oxidase-IN-1 (10 μM; 24 h) inhibits the mRNA expression of pro-inflammatory cytokines in BV2 cells. NADPH oxidase-IN-1 (1 nM-10 mM; 24 h) also inhibits activation and migration of BV2 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. RT-PCRCell Line: BV2 microglial cells Concentration: 10 μM Incubation Time: 24 hours Result: Inhibited the mRNA expression of pro-inflammatory cytokines (iNOS, IL-1β, TNFα) in BV2 cells. Cell Migration AssayCell Line: BV2 microglial cells Concentration: 10 μM Incubation Time: 24 hours Result: Ameliorated inflammatory response and migration of microglia.
In Vivo
NADPH oxidase-IN-1 (compound 11) (30 mg/kg; p.o.; daily for 4 wk) attenuates MPTP induced microglia activation and diminishes dopaminergic neuronal damage in Parkinson's disease (PD) mice model . NADPH oxidase-IN-1 is safe in both male and female mice following IV injection (10-300 mg/kg; single dose) and oral gavage (10-1000 mg/kg; single dose), respectively . Pharmacokinetic profile in rats Parameters C 0 (μg/mL) C max (μg/mL) t 1/2 (h) T max (h) AUC last (μg/h/mL) k e (1/h) V d (L) V d /F (L) Cl (L/h) Cl/F (L/h) F (%) IV (2 mg/kg) 1.70 0.79 0.468 1.32 0.211 0.217 PO (10 mg/kg) 0.609 5.01 [0.083-2] 1.31 0.170 1.99 0.341 56.0 PO (20 mg/kg) 0.783 6.13 [0.67-2] 4.16 0.159 1.82 0.217 88.9 MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
IC50& Target:NOX2 1.9 μM (IC 50 ) NOX4 2.47 μM (IC 50 )
规格
别名
NADPH 氧化酶-IN-1
英文名称
NADPH oxidase-IN-1
生化机理
NADPH 氧化酶-IN-1 是一种口服活性 NADPH 氧化酶（Nox）抑制剂，与神经元炎症有关。NADPH 氧化酶-IN-1 可穿过血脑屏障（BBB），抑制 Nox2 和 Nox4，其 IC 50 s 分别为 1.9 μM 和 2.47 μM。NADPH 氧化酶
储存条件
-20°C储存
运输条件
超低温运输
作用类型
抑制剂
名称和识别符
分子类型
小分子
分子量
373.51
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）