二代测序多样本接头引物试剂盒 I

货号: N665658
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级别和纯度: for Illumina
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规格
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价格
数量
24T
N665658-24T
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¥1,047.90
96T
N665658-96T
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¥3,747.90
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为什么选择此级别

,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

产品内容

N665658Component24 T96 TStorage
N665658AAdaptor for Illumina60 μL240 μL-20°C. Avoid freeze/thaw cycle.
N665658BUniversal Primer for Illumina30 μL120 μL-20°C. Avoid freeze/thaw cycle.
N665658CIndex 1–12 Primers for Illumina12×10 μL12×20 μL-20°C. Avoid freeze/thaw cycle.

产品简介

本试剂盒提供了二代测序建库中使用的adaptor与primer,专为illumina平台建库设计,index primer的设计可满足多重高通量测序时制备多个文库样本的需要,制备的文库可用于Illumina GAIIx,HiSacnSQ、HiSeq 2500/2000/1000和MiSeq sequencing等测序平台的测序。

自备仪器、试剂和耗材 

1.磁力架:建议使用DynaMagTM-2。 

2.DNA纯化回收试剂盒。 

3.DNA建库试剂盒。 

4.无水乙醇。 

5.反应管:建议使用低吸附的PCR管与1.5 ml离心管; 

枪头:建议使用高质量过滤枪头防止试剂盒、文库样本污染。

实验前准备及重要注意事项 

1.Adaptor为双链结构,请勿将其置于室温温度以上,以免发生解链、影响使用。 

2.Index primer开盖前请短暂离心,使液体收集到管底,以免不同引物间交叉污染。

使用流程

 

操作步骤 

二代测序多样本接头引物试剂盒使用方法请按照二代测序快速DNA建 库试剂盒protocol进行。 

Adaptor for Illumina: 

5´-/5Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´ 

5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´ 

Universal Primer for Illumina: 

5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T -3´ 

Index 1–12 Primers for Illumina:

Index Primers for Illumina

Index

Index 1 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATCGTGATGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

ATCACGAT

Index 2 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATACATCGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

CGATGTAT

Index 3 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATGCCTAAGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

TTAGGCAT

Index 4 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATTGGTCAGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

TGACCAAT

Index 5 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATCACTGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

ACAGTGAT

Index 6 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATATTGGCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

GCCAATAT

Index 7 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATGATCTGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

CAGATCAT

Index 8 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATTCAAGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

ACTTGAAT

Index 9 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATCTGATCGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

GATCAGAT

Index 10 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATAAGCTAGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

TAGCTTAT

Index 11 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATGTAGCCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

GGCTACAT

Index 12 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATTACAAGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

CTTGTAAT

Product content

N665658Component24 T96 TStorage
N665658AAdaptor for Illumina60 μL240 μL-20°C. Avoid freeze/thaw cycle.
N665658BUniversal Primer for Illumina30 μL120 μL-20°C. Avoid freeze/thaw cycle.
N665658CIndex 1–12 Primers for Illumina12×10 μL12×20 μL-20°C. Avoid freeze/thaw cycle.

Product Introduction

This kit provides the adaptor and primer used in second-generation sequencing library construction, designed for illumina platform library construction, index primer design can meet the needs of multiple high-throughput sequencing to prepare multiple library samples, the prepared library can be used for Illumina GAIIx, HiSacnSQ, HiSeq 2500/ 2000/1000 and MiSeq sequencing sequencing platform sequencing. 2000/1000 and MiSeq sequencing.

Provide your own instruments, reagents and consumables

1. Magnetic frame: DynaMagTM-2 is recommended.

2. DNA purification recovery kit.

3. DNA building kit.

4. Anhydrous ethanol.

5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;

6. Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.

Pre-experiment Preparation and Important Notes

1. Adaptor is a double chain structure, do not leave it above room temperature to avoid unraveling and affecting the use.

2. Please centrifuge the Index primer briefly before uncapping so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
Usage process

Operation steps

Please follow the protocol of the second-generation sequencing rapid DNA library building kit for the use of the second-generation sequencing multi sample connector primer kit.

Adaptor for Illumina:

5´-/5Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´

5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´

Universal Primer for Illumina:

5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T -3´

Index 1–12 Primers for Illumina:

Index Primers for Illumina

Index

Index 1 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATCGTGATGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

ATCACGAT

Index 2 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATACATCGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

CGATGTAT

Index 3 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATGCCTAAGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

TTAGGCAT

Index 4 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATTGGTCAGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

TGACCAAT

Index 5 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATCACTGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

ACAGTGAT

Index 6 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATATTGGCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

GCCAATAT

Index 7 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATGATCTGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

CAGATCAT

Index 8 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATTCAAGTGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

ACTTGAAT

Index 9 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATCTGATCGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

GATCAGAT

Index 10 Primer

for Illumina

5-CAAGCAGAAGACGGCATACGAGATAAGCTAGTGA

CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

TAGCTTAT

Index 11 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATGTAGCCGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

GGCTACAT

Index 12 Primer for Illumina

5-CAAGCAGAAGACGGCATACGAGATTACAAGGTGA CTGGAGTTCAGACGTGTGCTCTTCCGATC-s-T-3´

 

CTTGTAAT

规格

规格或纯度
for Illumina
英文名称
NGS Multiplex Oligos for Illumina (Index Primers Set I)
储存与运输
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
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