转座酶法二代测序多样本引物试剂盒 I(Illumina)

货号: N665968
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级别和纯度: 96 rxns
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规格
库存
价格
数量
96T
N665968-96T
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¥1,947.90
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为什么选择此级别

,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

产品内容 

N665968Component96 TStorage
N665968Adex N501-N508 Primers for Illumina    8×12 μL-20℃. Avoid freeze/thaw cycle.
N665968BIndex N701-N712 Primers for Illumina 12×8 μL-20℃. Avoid freeze/thaw cycle.

产品简介 

本试剂盒是转座酶法二代测序快速DNA建库试剂盒专用的配套试剂盒,专为 Illumina平台建库设计,其中包含8种N5端引物和12种N7端引物,可用于制备96种不同 的双端Index文库。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证,最大 程度上保证了文库构建的稳定性和重复性。制备的文库可用 H i S e q X-10/4000/2500/2000和MiSeq等Illumina平台测序。

自备仪器、试剂和耗材

1.磁力架:建议使用DynaMagTM-2。 

2.DNA纯化回收试剂盒:建议使用磁珠法DNA纯化回收试剂盒。 

3.DNA建库试剂盒:建议使用转座酶法二代测序快速DNA建库试剂盒。 

4.无水乙醇。 

5.反应管:建议使用低吸附的PCR管与1.5 ml离心管; 枪头:建议使用高质量过滤枪头防止试剂盒、文库样本污染。

  实验前准备及重要注意事项

  开盖前请短暂离心,使液体收集到管底,以免不同引物间交叉污染。 

操作步骤

  转座酶法二代测序多样本引物试剂盒使用方法请按照转座酶法二代测 序快速DNA建库试剂盒protocol进行。 

Index N501-N508 Primers for Illumina 

Index Primers for Illumina

Index

 

N501

5´-AATGATACGGCGACCACCGAGATCTA CACTAGATCGCTCGTCGGCAGCGTC-3´

 

TAGATCGC

 

N502

5´-AATGATACGGCGACCACCGAGATCTA

CACCTCTCTATTCGTCGGCAGCGTC-3´

 

CTCTCTAT

 

N503

5´-AATGATACGGCGACCACCGAGATCTA

CACTATCCTCTTCGTCGGCAGCGTC-3´

 

TATCCTCT

 

N504

5´-AATGATACGGCGACCACCGAGATCTA CACAGAGTAGATCGTCGGCAGCGTC-3´

 

AGAGTAGA

 

N505

5´-AATGATACGGCGACCACCGAGATCTA CACGTAAGGAGTCGTCGGCAGCGTC-3´

 

GTAAGGAG

 

N506

5´-AATGATACGGCGACCACCGAGATCTA CACACTGCATATCGTCGGCAGCGTC-3´

 

ACTGCATA

 

N507

5´-AATGATACGGCGACCACCGAGATCTA CACAAGGAGTATCGTCGGCAGCGTC-3´

 

AAGGAGTA

 

N508

5´-AATGATACGGCGACCACCGAGATCTA

CACCTAAGCCTTCGTCGGCAGCGTC-3´

 

CTAAGCCT

Index N701-N712 Primers for Illumina 

Index Primers for Illumina

Index

 

N701

5′-CAAGCAGAAGACGGCATACGAGAT

TCGCCTTAGTCTCGTGGGCTCGG-3′

 

TAAGGCGA

 

N702

5′-CAAGCAGAAGACGGCATACGAGAT

CTAGTACGGTCTCGTGGGCTCGG-3′

 

CGTACTAG

 

N703

5′-CAAGCAGAAGACGGCATACGAGAT

TTCTGCCTGTCTCGTGGGCTCGG-3′

 

AGGCAGAA

 

N704

5′-CAAGCAGAAGACGGCATACGAGAT

GCTCAGGAGTCTCGTGGGCTCGG-3′

 

TCCTGAGC

 

N705

5′-CAAGCAGAAGACGGCATACGAGAT

AGGAGTCCGTCTCGTGGGCTCGG-3′

 

GGACTCCT

 

N706

5′-CAAGCAGAAGACGGCATACGAGAT

CATGCCTAGTCTCGTGGGCTCGG-3′

 

TAGGCATG

 

N707

5′-CAAGCAGAAGACGGCATACGAGAT

GTAGAGAGGTCTCGTGGGCTCGG-3′

 

CTCTCTAC

 

N708

5′-CAAGCAGAAGACGGCATACGAGAT

CCTCTCTGGTCTCGTGGGCTCGG-3′

 

CAGAGAGG

 

N709

5′-CAAGCAGAAGACGGCATACGAGAT

AGCGTAGCGTCTCGTGGGCTCGG-3′

 

GCTACGCT

 

N710

5′-CAAGCAGAAGACGGCATACGAGAT

CAGCCTCGGTCTCGTGGGCTCGG-3′

 

CGAGGCTG

 

N711

5′-CAAGCAGAAGACGGCATACGAGAT

TGCCTCTTGTCTCGTGGGCTCGG-3′

 

AAGAGGCA

 

N712

5′-CAAGCAGAAGACGGCATACGAGAT

TCCTCTACGTCTCGTGGGCTCGG-3′

 

GTAGAGGA


Products content

N665968Component96 TStorage
N665968Adex N501-N508 Primers for Illumina    8×12 μL-20℃. Avoid freeze/thaw cycle.
N665968BIndex N701-N712 Primers for Illumina 12×8 μL-20℃. Avoid freeze/thaw cycle.

Products Introduction

This kit is a companion kit for the transposase-based second-generation sequencing Rapid DNA Library Construction Kit, designed for Illumina platform library construction, which contains 8 primers at the N5 end and 12 primers at the N7 end, which can be used to prepare 96 different bipartite Index libraries. All reagents provided in the kit have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The prepared libraries can be sequenced on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq.

 

Provide your own instruments, reagents and consumables

1. Magnetic frame: DynaMagTM-2 is recommended.

2. DNA purification and recovery kit: It is recommended to use Kangwei DNA purification and recovery kit by magnetic bead method.

3. DNA building kit: It is recommended to use the Kangwei Century transposase method second-generation sequencing rapid DNA building kit.

4. Anhydrous ethanol.

5. Reaction tubes: It is recommended to use low adsorption PCR tubes and 1.5 ml centrifuge tubes; tips: It is recommended to use high-quality filtration tips to prevent contamination of reagent kits and library samples.

 

Pre-experiment Preparation and Important Notes

Please centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.

For the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.

Index N501-N508 Primers for Illumina


Index N701-N712 Primers for Illumina


规格

规格或纯度
96 rxns
英文名称
NGS TP Index Kit for Illumina (Index Primers Set I)
储存与运输
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
溶液计算器