计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
产品内容
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产品简介
本试剂盒是转座酶法二代测序快速DNA建库试剂盒专用的配套试剂盒,专为 Illumina平台建库设计,其中包含8种N5端引物和12种N7端引物,可用于制备96种不同 的双端Index文库。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证,最大 程度上保证了文库构建的稳定性和重复性。制备的文库可用 H i S e q X-10/4000/2500/2000和MiSeq等Illumina平台测序。
自备仪器、试剂和耗材
1.磁力架:建议使用DynaMagTM-2。
2.DNA纯化回收试剂盒:建议使用磁珠法DNA纯化回收试剂盒。
3.DNA建库试剂盒:建议使用转座酶法二代测序快速DNA建库试剂盒。
4.无水乙醇。
5.反应管:建议使用低吸附的PCR管与1.5 ml离心管; 枪头:建议使用高质量过滤枪头防止试剂盒、文库样本污染。
实验前准备及重要注意事项
开盖前请短暂离心,使液体收集到管底,以免不同引物间交叉污染。
操作步骤
转座酶法二代测序多样本引物试剂盒使用方法请按照转座酶法二代测 序快速DNA建库试剂盒protocol进行。
Index N501-N508 Primers for Illumina
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Index N701-N712 Primers for Illumina
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Products content
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Products Introduction
This kit is a companion kit for the transposase-based second-generation sequencing Rapid DNA Library Construction Kit, designed for Illumina platform library construction, which contains 8 primers at the N5 end and 12 primers at the N7 end, which can be used to prepare 96 different bipartite Index libraries. All reagents provided in the kit have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The prepared libraries can be sequenced on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq.
Provide your own instruments, reagents and consumables
1. Magnetic frame: DynaMagTM-2 is recommended.
2. DNA purification and recovery kit: It is recommended to use Kangwei DNA purification and recovery kit by magnetic bead method.
3. DNA building kit: It is recommended to use the Kangwei Century transposase method second-generation sequencing rapid DNA building kit.
4. Anhydrous ethanol.
5. Reaction tubes: It is recommended to use low adsorption PCR tubes and 1.5 ml centrifuge tubes; tips: It is recommended to use high-quality filtration tips to prevent contamination of reagent kits and library samples.
Pre-experiment Preparation and Important Notes
Please centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
For the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.
Index N501-N508 Primers for Illumina

Index N701-N712 Primers for Illumina
