产品内容
| N665978 | Component | 384 T | Storage | | N665978A | Index N502-N522 Primers for Illumina | 16×24 μL | -20℃. Avoid freeze/thaw cycle. | | N665978B | Index N701-N729 Primers for Illumina | 24×16 μL | -20℃. Avoid freeze/thaw cycle. |
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产品简介
本试剂盒是转座酶法二代测序快速DNA建库试剂盒专用的配套试剂盒,专为
Illumina平台建库设计,其中包含16种N5端引物和24种N7端引物,可用于制备384种
不同的双端Index文库。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证,
最大程度上保证了文库构建的稳定性和重复性。制备的文库可用于HiSeq X-10/4000/
2500/2000和MiSeq等Illumina平台测序。
自备仪器、试剂和耗材
1.磁力架:建议使用DynaMagTM-2。
2.DNA纯化回收试剂盒:建议使用磁珠法DNA纯化回收试剂盒。
3.DNA建库试剂盒:建议使用世纪转座酶法二代测序快速DNA建库试剂盒。
4.无水乙醇。
5.反应管:建议使用低吸附的PCR管与1.5 ml离心管;
枪头:建议使用高质量过滤枪头防止试剂盒、文库样本污染。
实验前准备及重要注意事项
开盖前请短暂离心,使液体收集到管底,以免不同引物间交叉污染。
操作步骤
转座酶法二代测序多样本引物试剂盒使用方法请按照转座酶法二代测
序快速DNA建库试剂盒protocol进行。
Index N502-N522 Primers for Illumina
| Index Primers for IIlumina | Index | N502 | 5'-AATGATACGGCGACCACCGAGATCTA CACCTCTCTATTCGTCGGCAGCGTC-3' | CTCTCTAT | N503 | 5'-AATGATACGGCGACCACCGAGATCTA CACTATCCTCTTCGTCGGCAGCGTC-3' | TATCCTCT | N505 | 5'-AATGATACGGCGACCACCGAGATCTA CACGTAAGGAGTCGTCGGCAGCGTC-3' | GTAAGGAG | N506 | 5'-AATGATACGGCGACCACCGAGATCTA CACACTGCATATCGTCGGCAGCGTC-3' | ACTGCATA | N507 | 5'-AATGATACGGCGACCACCGAGATCTA CACAAGGAGTATCGTCGGCAGCGTC-3' | AAGGAGTA | N508 | 5'-AATGATACGGCGACCACCGAGATCTA CACCTAAGCCTTCGTCGGCAGCGTC-3' | CTAAGCCT | N510 | 5'-AATGATACGGCGACCACCGAGATCTA CACCGTCTAATTCGTCGGCAGCGTC-4' | CGTCTAAT | N511 | 5'-AATGATACGGCGACCACCGAGATCTA CACTCTCTCCGTCGTCGGCAGCGTC-5' | TCTCTCCG |
| Index Primers for IlIum ina | Index | N513 | 5'-AATGATACGGCGACCACCGAGATCTA CACTCGACTAGTCGTCGGCAGCGTC-6' | TCGACTAG | N515 | 5'-AATGATACGGCGACCACCGAGATCTA CACTTCTAGCTTCGTCGGCAGCGTC-7' | TTCTAGCT | N516 | 5'-AATGATACGGCGACCACCGAGATCTA CACCCTAGAGTTCGTCGGCAGCGTC-8' | CCTAGAGT | N517 | 5'-AATGATACGGCGACCACCGAGATCTA CACGCGTAAGATCGTCGGCAGCGTC-3' | GCGTAAGA | N518 | 5'-AATGATACGGCGACCACCGAGATCTA CACCTATTAAGTCGTCGGCAGCGTC-4' | CTATTAAG | N520 | 5'-AATGATACGGCGACCACCGAGATCTA CACAAGGCTATTCGTCGGCAGCGTC-5' | AAGGCTAT | N521 | 5'-AATGATACGGCGACCACCGAGATCTA CACGAGCCTTATCGTCGGCAGCGTC-6' | GAGCCTTA | N522 | 5'-AATGATACGGCGACCACCGAGATCTA CACTTATGCGATCGTCGGCAGCGTC-7' | TTATGCGA |
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Index N701-N729 Primers for IIlumina
| Index Primers for IlIum ina | Index | N701 | 5'-CAAGCAGAAGACGGCATACGAGAT TCGCCTTAGTCTCGTGGGCTCGG-3' | TAAGGCGA | N702 | 5'-CAAGCAGAAGACGGCATACGAGAT CTAGTACGGTCTCGTGGGCTCGG-3' | CGTACTAG | N703 | 5'-CAAGCAGAAGACGGCATACGAGAT TTCTGCCTGTCTCGTGGGCTCGG-3' | AGGCAGAA | N704 | 5'-CAAGCAGAAGACGGCATACGAGAT GCTCAGGAGTCTCGTGGGCTCGG-3' | TCCTGAGC | N705 | 5'-CAAGCAGAAGACGGCATACGAGAT AGGAGTCCGTCTCGTGGGCTCGG-3' | GGACTCCT | N706 | 5'-CAAGCAGAAGACGGCATACGAGAT CATGCCTAGTCTCGTGGGCTCGG-3' | TAGGCATG | N707 | 5'-CAAGCAGAAGACGGCATACGAGAT GTAGAGAGGTCTCGTGGGCTCGG-3' | CTCTCTAC | N710 | 5'-CAAGCAGAAGACGGCATACGAGAT CAGCCTCGGTCTCGTGGGCTCGG-3' | CGAGGCTG |
| Index Primers for IIlumina | Index | N711 | 5'-CAAGCAGAAGACGGCATACGAGAT TGCCTCTTGTCTCGTGGGCTCGG-3' | AAGAGGCA | N712 | 5'-CAAGCAGAAGACGGCATACGAGAT TCCTCTACGTCTCGTGGGCTCGG-3' | GTAGAGGA | N714 | 5'-CAAGCAGAAGACGGCATACGAGAT TCATGAGCGTCTCGTGGGCTCGG-3' | GCTCATGA | N715 | 5'-CAAGCAGAAGACGGCATACGAGAT CCTGAGATGTCTCGTGGGCTCGG-3' | | | N716 | 5'-CAAGCAGAAGACGGCATACGAGAT TAGCGAGTGTCTCGTGGGCTCGG-3' | ACTCGCTA | N718 | 5'-CAAGCAGAAGACGGCATACGAGAT GTAGCTCCGTCTCGTGGGCTCGG-3' | GGAGCTAC | N719 | 5'-CAAGCAGAAGACGGCATACGAGAT TACTACGCGTCTCGTGGGCTCGG-3' | GCGTAGTA | N720 | 5CAAGCAGAAGACGGCATACGAGAT AGGCTCCGGTCTCGTGGGCTCGG-3' | CGGAGCCT | N721 | 5'-CAAGCAGAAGACGGCATACGAGAT GCAGCGTAGTCTCGTGGGCTCGG-3' | TACGCTGC | N722 | 5'-CAAGCAGAAGACGGCATACGAGAT CTGCGCATGTCTCGTGGGCTCGG-3' | ATGCGCAG | N723 | 5'-CAAGCAGAAGACGGCATACGAGAT GAGCGCTAGTCTCGTGGGCTCGG-3' | TAGCGCTC | N724 | 5'-CAAGCAGAAGACGGCATACGAGAT CGCTCAGTGTCTCGTGGGCTCGG-3' | ACTGAGCG | N726 | 5'-CAAGCAGAAGACGGCATACGAGAT GTCTTAGGGTCTCGTGGGCTCGG-3' | CCTAAGAC | N727 | 5'-CAAGCAGAAGACGGCATACGAGAT ACTGATCGGTCTCGTGGGCTCGG-3' | CGATCAGT | N728 | 5'-CAAGCAGAAGACGGCATACGAGAT TAGCTGCAGTCTCGTGGGCTCGG-3' | TGCAGCTA | N729 | 5'-CAAGCAGAAGACGGCATACGAGAT GACGTCGAGTCTCGTGGGCTCGG-3' | TCGACGTC |
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Products content
| N665978 | Component | 384 T | Storage | | N665978A | Index N502-N522 Primers for Illumina | 16×24 μL | -20℃. Avoid freeze/thaw cycle. | | N665978B | Index N701-N729 Primers for Illumina | 24×16 μL | -20℃. Avoid freeze/thaw cycle. |
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Products Introduction
This kit is a companion to the transposase-based rapid DNA library construction kit, designed for Illumina platform library construction. It contains 16 N5 primers and 24 N7 primers, which can be used to prepare 384 different bipartite Index libraries. All reagents provided in the kit have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The libraries can be used for sequencing on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq.
Provide your own instruments, reagents and consumables
1. Magnetic frame: DynaMagTM-2 is recommended.
2. DNA purification and recovery kit: It is recommended to use Kangwei DNA purification and recovery kit by magnetic bead method.
3. DNA building kit: It is recommended to use the Kangwei Century transposase method second-generation sequencing rapid DNA building kit.
4. Anhydrous ethanol.
5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;
Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.
Pre-experiment Preparation and Important Notes
Please centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
procedure
For the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.
Index N502-N522 Primers for Illumina


Index N701-N729 Primers for Illumina


