计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
|
产品简介
本试剂盒提供了MGI平台二代测序所需的Adaptor及Index primer Mix，专为MGI测
序平台设计。Adaptor在MGI平台原基础上进行了改进，改良后的Adaptor为通用型，
没有Barcode序列，便于自动化操作，含有Universal primer及Index primer结合位点，
通过Index primer引入Barcode序列。改进后的接头可显著提高文库连接效率、转化效
率及扩增效率。本套系试剂盒共有三组，分别是二代测序多样本接头引物
试剂盒Ⅰ（MGI平台Barcode 1-48）、二代测序多样本接头引物试剂盒Ⅱ
（MGI平台Barcode 49-96） 、二代测序多样本接头引物试剂盒Ⅲ（MGI平
台Barcode 97-128）。涵盖MGI测序平台1-128号Barcode序列。制备的文库可用于MGI
平台BGISEQ-500RS (PE50/PE100/PE150)、MGISEQ-2000RS (PE100/PE150)、
MGISEQ-200RS (PE100)等测序仪的测序。
自备仪器、试剂和耗材
1．磁力架：建议使用DynaMagTM-2。
2．DNA纯化回收试剂盒：建议使用磁珠法DNA纯化回收试剂盒。
3．DNA建库试剂盒：建议使用cfDNA文库快速制备试剂盒（MGI平台），DNA文库快速制备试剂盒，含片段化酶的二代测序 快速DNA建库试剂盒。
4．无水乙醇。
5．反应管：建议使用低吸附的PCR管与1.5 ml离心管； 枪头：建议使用高质量过滤枪 头防止试剂盒、文库样本污染。
实验前准备及重要注意事项
1．Adaptor为双链结构，请勿将其置于室温温度以上，以免发生解链、影响使用。
2．Index primer Mix开盖前请短暂离心，使液体收集到管底，以免不同引物间交叉污染。
操作流程
操作步骤
二代测序多样本接头引物试剂Ⅲ使用方法按照DNA文库快速制备试剂盒（MGI平
台）protocol进行。
Index primer Mix 是由Universal primer和Index primer配制而成，其中Index primer
有32种。可以根据不同混合测序策略，每个样品加入不同的 Index，或一个样本加入一
套Index，要求最终混合后的文库保证所有Index都以套为单位凑齐。Index 97-128中，
8个为一套，分别为97-104，105-112，113-120及121-128。
Table: Barcode 97-128 for MGI
|
Products content
|
Products Introduction
This kit provides the Adaptor and Index primer Mix for MGI platform second generation sequencing, which is specially designed for MGI sequencing platform, the Adaptor is improved on the basis of the original MGI platform, the improved Adaptor is a universal type without Barcode sequence, which is convenient for automation, and it contains Universal primer and Index primer binding site, which is introduced into Barcode sequence by Index primer. It contains Universal primer and Index primer binding site, and the Barcode sequence is introduced by Index primer. The improved connector can significantly improve the library ligation efficiency, transformation efficiency and amplification efficiency. There are three sets of this kit, namely: Second Generation Sequencing Multi-sample Junction Primer Kit I (Barcode 1-48 of MGI platform), Second Generation Sequencing Multi-sample Junction Primer Kit II (Barcode 49-96 of MGI platform), and Second Generation Sequencing Multi-sample Junction Primer Kit III (Barcode 97-128 of MGI platform). These libraries cover Barcode 1-128 of MGI sequencing platform. The prepared libraries can be used for sequencing on BGISEQ-500RS (PE50/PE100/PE150), MGISEQ-2000RS (PE100/PE150), MGISEQ-200RS (PE100) and other sequencers of MGI platform.
Provide your own instruments, reagents and consumables
1．Magnetic frame: DynaMagTM-2 is recommended.
2．DNA Purification and Recovery Kit: We recommend the use of the Kangwei Magnetic Bead Method DNA Purification and Recovery Kit.
DNA Library Building Kit: Kangwei cfDNA Library Rapid Preparation Kit (MGI platform), DNA Library Rapid Preparation Kit, Second Generation Sequencing with Fragmentation Enzyme Rapid DNA Library Building Kit is recommended.
3．Anhydrous ethanol.
4．Reaction Tubes: It is recommended to use PCR tubes with low adsorption and 1.5 ml centrifuge tubes; Tips: It is recommended to use high-quality filter tips to prevent contamination of kits and libraries.
Pre-experiment Preparation and Important Notes
1.Adaptor is a double-chain structure, please do not leave it above room temperature to avoid unchaining and affecting the use.
2．Please centrifuge the Index primer Mix briefly before uncapping so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
workflow
procedure
The second-generation sequencing multi-sample junction primer kit II was used according to the DNA Library Rapid Preparation Kit (MGI platform) protocol.
Index primer Mix is composed of Universal primer and Index primer, of which there are 32 Index primers. According to different mixing strategies, different Indexes can be added to each sample, or one set of Indexes can be added to each sample, and the final mixed library is required to ensure that all Indexes are assembled in a set. 8 of Indexes 97-128 are in a set, which are 97-104, 105-112, 113-120 and 121-128 respectively.
Table: Barcode 97-128 for MGI
规格
规格或纯度
64 rxns
英文名称
NGS Multiplex Oligos for MGI (Index primer mix Set III)
储存与运输
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）