N666055	Component	96 T	Storage
N666055A	Adaptor for Illumina	480 μL	-20℃. Avoid freeze/thaw cycle.
N666055B	i7 Index Primers D701-D712	12×20 μL	-20℃. Avoid freeze/thaw cycle.
N666055C	i5 Index Primers D501–D508	8×30 μL	-20℃. Avoid freeze/thaw cycle.

产品简介

NGS Combinatorial Dual Index Primers Kit for Illumina (Set I)是 Illumina 高通量测序平台文库构建专用index接头引物试剂盒。本试剂盒包含通用接头DNA Adaptor for Illumina、8 种 i5 Index Primers 以及 12 种 i7 Index Primers，配合Fast DNA Library Prep Set for Illumina & MGI和NGS Frag Fast DNA Library Prep Set for Illumina使用，可用于构建多至 96 种不同组合的双端Index标记二代测序文库。制备的文库可用于NovaSeq，MiSeq，HiSeq 2000/2500/3000/4000，MiniSeq 和NextSeq等测序平台的测序。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证，最大程度上保证了文库构建的稳定性和重复性。

适用范围

适用于Illumina高通量测序平台双端 Index标记文库构建。推荐与Fast DNA Library Prep Set for Illumina & MGI和NGS Frag Fast DNA Library Prep Set for Illumina配合使用。

产品组分

用途	组分	(96 rxn)	(192 rxn)
Illumina通用接头	DNA Adaptor for Illumina	480 μl	2 × 480 μl
i7 Index Primer	D701	20 μl	40 μl
i7 Index Primer	D702	20 μl	40 μl
i7 Index Primer	D703	20 μl	40 μl
i7 Index Primer	D704	20 μl	40 μl
i7 Index Primer	D705	20 μl	40 μl
i7 Index Primer	D706	20 μl	40 μl
i7 Index Primer	D707	20 μl	40 μl
i7 Index Primer	D708	20 μl	40 μl
i7 Index Primer	D709	20 μl	40 μl
i7 Index Primer	D710	20 μl	40 μl
i7 Index Primer	D711	20 μl	40 μl
i7 Index Primer	D712	20 μl	40 μl
i5 Index Primer	D501	30 μl	60 μl
i5 Index Primer	D502	30 μl	60 μl
i5 Index Primer	D503	30 μl	60 μl
i5 Index Primer	D504	30 μl	60 μl
i5 Index Primer	D505	30 μl	60 μl
i5 Index Primer	D506	30 μl	60 μl
i5 Index Primer	D507	30 μl	60 μl
i5 Index Primer	D508	30 μl	60 μl

注：单个文库DNA Adapter for Illumina的使用量依据起始模板投入量而定， i7 Index Primers 和i5 Index Primers使用量均为2.5 μl。

序列信息

DNA Adapter for Illumina

5´-/Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´

5´-ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´

(*表示硫代, Phos表示磷酸化)

i5 Index Primers 5´-AATGATACGGCGACCACCGAGATCTACAC [i5]ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´
i7 Index Primers

5´-CAAGCAGAAGACGGCATACGAGAT

[i7]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC*T-3´

*表示硫代)

[i5]表示8 bp i5 Index序列，[i7]表示8 bp i7 Index序列。

各引物对应的Index名称、引物中包含的Index序列以及测序时Sample Sheet中输入的 Index序列信息如下表所示：

		i5 Bases for Sample Sheet	i5 Bases for Sample Sheet
i5 Index Name	Index Bases in i5 Index Primer	NovaSeq, MiSeq, HiSeq 2000/2500	MiniSeq, NextSeq, HiSeq 3000/4000
D501	TATAGCCT	TATAGCCT	AGGCTATA
D502	ATAGAGGC	ATAGAGGC	GCCTCTAT
D503	CCTATCCT	CCTATCCT	AGGATAGG
D504	GGCTCTGA	GGCTCTGA	TCAGAGCC
D505	AGGCGAAG	AGGCGAAG	CTTCGCCT
D506	TAATCTTA	TAATCTTA	TAAGATTA
D507	CAGGACGT	CAGGACGT	ACGTCCTG
D508	GTACTGAC	GTACTGAC	GTCAGTAC

i7 Index Name	Index Bases in i7 Index Primer	i7 Bases for Sample Sheet
D701	CGAGTAAT	ATTACTCG
D702	TCTCCGGA	TCCGGAGA
D703	AATGAGCG	CGCTCATT
D704	GGAATCTC	GAGATTCC
D705	TTCTGAAT	ATTCAGAA
D706	ACGAATTC	GAATTCGT
D707	AGCTTCAG	CTGAAGCT
D708	GCGCATTA	TAATGCGC
D709	CATAGCCG	CGGCTATG
D710	TTCGCGGA	TCCGCGAA
D711	GCGCGAGA	TCTCGCGC
D712	CTATCGCT	AGCGATAG

建库流程和文库结构

本试剂盒与Fast DNA Library Prep Set for Illumina & MGI和NGS Frag Fast DNA Library Prep Set for Illumina配合使用，文库构建流程示意图如下：

构建好的文库结构如下

5’- AATGATACGGCGACCACCGAGATCTACAC [i5] ACACTCTTTCCCTACACGACGCTCTTCCGATCT [DNA insert] AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC [i7] ATCTCGTATGCCGTCTTCTGCTTG-3’ i5：i5 index, 8 bases i7：i7 index, 8 bases DNA insert：插入的目标测序序列

N666055	Component	96 T	Storage
N666055A	Adaptor for Illumina	480 μL	-20℃. Avoid freeze/thaw cycle.
N666055B	i7 Index Primers D701-D712	12×20 μL	-20℃. Avoid freeze/thaw cycle.
N666055C	i5 Index Primers D501–D508	8×30 μL	-20℃. Avoid freeze/thaw cycle.

Products Introduction

The NGS Combinatorial Dual Index Primers Kit for Illumina (Set I) is an index primer kit for library construction on the Illumina high-throughput sequencing platform. This kit contains the Universal Junction DNA Adaptor for Illumina, 8 i5 Index Primers, and 12 i7 Index Primers for use with the Fast DNA Library Prep Set for Illumina & MGI and the NGS Frag Fast DNA Library Prep Set for Illumina. Library Prep Set for Illumina, 8 i5 Index Primers, and 12 i7 Index Primers can be used with the Fast DNA Library Prep Set for Illumina & MGI and the NGS Frag Fast DNA Library Prep Set for Illumina to build up to 96 different combinations of bipartite Index-tagged second generation sequencing libraries. The prepared libraries can be used for sequencing on NovaSeq, MiSeq, HiSeq 2000/2500/3000/4000, MiniSeq and NextSeq sequencing platforms. All the reagents provided in the kit have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of the library construction.

Scope of application

For use with Illumina High-Throughput Sequencing Platform Double-Ended Index Labeled Library Construction. Recommended for use with Fast DNA Library Prep Set for Illumina & MGI and NGS Frag Fast DNA Library Prep Set for Illumina.

product components

Note: The amount of individual library DNA Adapter for Illumina used depends on the amount of starting template input. i7 Index Primers and i5 Index Primers both use 2.5 μl.

Sequence information

DNA Adapter for Illumina

5´-/Phos/ GATCGGAAGAGCACACGTCTGAACTCCAGT*C -3´

5´-ACACTCTTTCCCTACACGACGCTCTCTTCCGATC*T-3´

(* denotes thiolation, Phos denotes phosphorylation)

i5 Index Primers 5´-AATGATACGGCGACCACCGAGATCTACAC [i5]ACACTCTTTCCCTACACGACGCTCTTCCGATC*T-3´

i7 Index Primers

5´-CAAGCAGAAGACGGCATACGAGAT

[i7]GTGACTGGAGTTCAGACGTGTGCTCTTCCGATC*T-3´.

* denotes thio)

[i5] denotes an 8 bp i5 Index sequence and [i7] denotes an 8 bp i7 Index sequence.

The Index name corresponding to each primer, the Index sequence contained in the primer, and the Index entered in the Sample Sheet during sequencing.

Library building process and library structure

This kit is used in conjunction with Fast DNA Library Prep Set for Illumina & MGI and NGS Frag Fast DNA Library Prep Set for Illumina, and the library construction process is summarized below:

The structure of the constructed library is as follows

5'- AATGATACGGCGACCACCGAGATCTACAC [i5] ACACTCTTTCCCTACACGACGCTCTTCCGATCT [DNA insert] AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC [i7] ATCTCGTATGCCGTCTTCTGCTTG-3' i5: i5 index, 8 bases i7: i7 index, 8 bases DNA insert: inserted target sequencing sequence

规格

别名

Illumina 的 NGS 组合双索引引物试剂盒(套件 I)

英文名称

NGS Combinatorial Dual Index Primers Kit for Illumina (Set I)

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

产品线

生物产品

名称和识别符

分子类型

试剂盒

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

ngs combinatorial dual index primers kit for illumina (set i).pdf

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

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