产品内容
| N669983 | Component | 240 T | Storage | | N669983A | Index N501 Primers for Illumina | 240 μL | -20℃. Avoid freeze/thaw cycle. | | N669983B | Index N973-N996 Primers for Illumina | 24×10 μL | -20℃. Avoid freeze/thaw cycle. |
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产品简介
本试剂盒是转座酶法二代测序快速DNA建库试剂盒专用的配套试剂盒，专为
Illumina平台建库设计，每个试剂盒包含1种N5端引物和24种N7端引物，可用于制备24
种不同的单端Index文库。试剂盒中提供的所有试剂都经过严格的质量控制和功能验证，
最大程度上保证了文库构建的稳定性和重复性。制备的文库可用于HiSeq X-10/4000/2500
/2000和MiSeq等Illumina平台测序。
自备仪器、试剂和耗材
1．磁力架：建议使用DynaMagTM-2 。
2．DNA纯化回收试剂盒：建议使用磁珠法DNA纯化回收试剂盒。
3．DNA建库试剂盒：建议使用世纪转座酶法二代测序快速DNA建库试剂盒。
4．无水乙醇。
5．反应管：建议使用低吸附的PCR管与1.5 ml离心管；
枪头：建议使用高质量过滤枪头防止试剂盒、文库样本污染。
实验前准备及重要注意事项
开盖前请短暂离心，使液体收集到管底，以免不同引物间交叉污染。
操作步骤
转座酶法二代测序多样本引物试剂盒使用方法请按照转座酶法二代测
序快速DNA建库试剂盒protocol进行。
Index N501 Primer for Illumina
| Index Primer for Illumina | N501 | 5′-AATGATACGGCGACCACCGAGATCTACACTAGATCGCTCGTCGGCAGCGTC-3′ |
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Index N901-N996 Primer for Illumina
| Index Primers for Illumina | Index | N901 | 5′-CAAGCAGAAGACGGCATACGAGAT AACGTGATGTCTCGTGGGCTCGG-3′ | ATCACGTT | N902 | 5′-CAAGCAGAAGACGGCATACGAGAT AAACATCGGTCTCGTGGGCTCGG-3′ | CGATGTTT | N903 | 5′-CAAGCAGAAGACGGCATACGAGAT ATGCCTAAGTCTCGTGGGCTCGG-3′ | TTAGGCAT | N904 | 5′-CAAGCAGAAGACGGCATACGAGAT AGTGGTCAGTCTCGTGGGCTCGG-3′ | TGACCACT | N905 | 5′-CAAGCAGAAGACGGCATACGAGAT ACCACTGTGTCTCGTGGGCTCGG-3′ | ACAGTGGT | N906 | 5′-CAAGCAGAAGACGGCATACGAGAT ACATTGGCGTCTCGTGGGCTCGG-3′ | GCCAATGT |
| Index Primers for Illumina | Index | N907 | 5′-CAAGCAGAAGACGGCATACGAGAT CAGATCTGGTCTCGTGGGCTCGG-3′ | CAGATCTG | N908 | 5′-CAAGCAGAAGACGGCATACGAGAT CATCAAGTGTCTCGTGGGCTCGG-3′ | ACTTGATG | N909 | 5′-CAAGCAGAAGACGGCATACGAGAT CGCTGATCGTCTCGTGGGCTCGG-3′ | GATCAGCG | N910 | 5′-CAAGCAGAAGACGGCATACGAGAT ACAAGCTAGTCTCGTGGGCTCGG-3′ | TAGCTTGT | N911 | 5′-CAAGCAGAAGACGGCATACGAGAT CTGTAGCCGTCTCGTGGGCTCGG-3′ | GGCTACAG | N912 | 5′-CAAGCAGAAGACGGCATACGAGAT AGTACAAGGTCTCGTGGGCTCGG-3′ | CTTGTACT | N913 | 5′-CAAGCAGAAGACGGCATACGAGAT AACAACCAGTCTCGTGGGCTCGG-3′ | TGGTTGTT | N914 | 5′-CAAGCAGAAGACGGCATACGAGAT AACCGAGAGTCTCGTGGGCTCGG-3′ | TCTCGGTT | N915 | 5′-CAAGCAGAAGACGGCATACGAGAT AACGCTTAGTCTCGTGGGCTCGG-3′ | TAAGCGTT | N916 | 5′-CAAGCAGAAGACGGCATACGAGAT AAGACGGAGTCTCGTGGGCTCGG-3′ | TCCGTCTT | N917 | 5′-CAAGCAGAAGACGGCATACGAGAT AAGGTACAGTCTCGTGGGCTCGG-3′ | TGTACCTT | N918 | 5′-CAAGCAGAAGACGGCATACGAGAT ACACAGAAGTCTCGTGGGCTCGG-3′ | TTCTGTGT | N919 | 5′-CAAGCAGAAGACGGCATACGAGAT ACAGCAGAGTCTCGTGGGCTCGG-3′ | TCTGCTGT | N920 | 5′-CAAGCAGAAGACGGCATACGAGAT ACCTCCAAGTCTCGTGGGCTCGG-3′ | TTGGAGGT | N921 | 5′-CAAGCAGAAGACGGCATACGAGAT ACGCTCGAGTCTCGTGGGCTCGG-3′ | TCGAGCGT | N922 | 5′-CAAGCAGAAGACGGCATACGAGAT ACGTATCAGTCTCGTGGGCTCGG-3′ | TGATACGT | N923 | 5′-CAAGCAGAAGACGGCATACGAGAT ACTATGCAGTCTCGTGGGCTCGG-3′ | TGCATAGT | N924 | 5′-CAAGCAGAAGACGGCATACGAGAT AGAGTCAAGTCTCGTGGGCTCGG-3′ | TTGACTCT |
| Index Primers for Illumina | Index | N925 | 5′-CAAGCAGAAGACGGCATACGAGAT AGATCGCAGTCTCGTGGGCTCGG-3′ | TGCGATCT | N926 | 5′-CAAGCAGAAGACGGCATACGAGAT AGCAGGAAGTCTCGTGGGCTCGG-3′ | TTCCTGCT | N927 | 5′-CAAGCAGAAGACGGCATACGAGAT AGTCACTAGTCTCGTGGGCTCGG-3′ | TAGTGACT | N928 | 5′-CAAGCAGAAGACGGCATACGAGAT ATCCTGTAGTCTCGTGGGCTCGG-3′ | TACAGGAT | N929 | 5′-CAAGCAGAAGACGGCATACGAGAT ATTGAGGAGTCTCGTGGGCTCGG-3′ | TCCTCAAT | N930 | 5′-CAAGCAGAAGACGGCATACGAGAT CAACCACAGTCTCGTGGGCTCGG-3′ | TGTGGTTG | N931 | 5′-CAAGCAGAAGACGGCATACGAGAT GACTAGTAGTCTCGTGGGCTCGG-3′ | TACTAGTC | N932 | 5′-CAAGCAGAAGACGGCATACGAGAT CAATGGAAGTCTCGTGGGCTCGG-3′ | TTCCATTG | N933 | 5′-CAAGCAGAAGACGGCATACGAGAT CACTTCGAGTCTCGTGGGCTCGG-3′ | TCGAAGTG | N934 | 5′-CAAGCAGAAGACGGCATACGAGAT CAGCGTTAGTCTCGTGGGCTCGG-3′ | TAACGCTG | N935 | 5′-CAAGCAGAAGACGGCATACGAGAT CATACCAAGTCTCGTGGGCTCGG-3′ | TTGGTATG | N936 | 5′-CAAGCAGAAGACGGCATACGAGAT CCAGTTCAGTCTCGTGGGCTCGG-3′ | TGAACTGG | N937 | 5′-CAAGCAGAAGACGGCATACGAGAT CCGAAGTAGTCTCGTGGGCTCGG-3′ | TACTTCGG | N938 | 5′-CAAGCAGAAGACGGCATACGAGAT CCGTGAGAGTCTCGTGGGCTCGG-3′ | TCTCACGG | N939 | 5′-CAAGCAGAAGACGGCATACGAGAT CCTCCTGAGTCTCGTGGGCTCGG-3′ | TCAGGAGG | N940 | 5′-CAAGCAGAAGACGGCATACGAGAT CGAACTTAGTCTCGTGGGCTCGG-3′ | TAAGTTCG | N941 | 5′-CAAGCAGAAGACGGCATACGAGAT CGACTGGAGTCTCGTGGGCTCGG-3′ | TCCAGTCG | N942 | 5′-CAAGCAGAAGACGGCATACGAGAT CGCATACAGTCTCGTGGGCTCGG-3′ | TGTATGCG |
| Index Primers for Illumina | Index | N943 | 5′-CAAGCAGAAGACGGCATACGAGAT CTCAATGAGTCTCGTGGGCTCGG-3′ | TCATTGAG | N944 | 5′-CAAGCAGAAGACGGCATACGAGAT CTGAGCCAGTCTCGTGGGCTCGG-3′ | TGGCTCAG | N945 | 5′-CAAGCAGAAGACGGCATACGAGAT CTGGCATAGTCTCGTGGGCTCGG-3′ | TATGCCAG | N946 | 5′-CAAGCAGAAGACGGCATACGAGAT GAATCTGAGTCTCGTGGGCTCGG-3′ | TCAGATTC | N947 | 5′-CAAGCAGAAGACGGCATACGAGAT CAAGACTAGTCTCGTGGGCTCGG-3′ | TAGTCTTG | N948 | 5′-CAAGCAGAAGACGGCATACGAGAT GAGCTGAAGTCTCGTGGGCTCGG-3′ | TTCAGCTC | N949 | 5′-CAAGCAGAAGACGGCATACGAGAT GATAGACAGTCTCGTGGGCTCGG-3′ | TGTCTATC | N950 | 5′-CAAGCAGAAGACGGCATACGAGAT GCCACATAGTCTCGTGGGCTCGG-3′ | TATGTGGC | N951 | 5′-CAAGCAGAAGACGGCATACGAGAT GCGAGTAAGTCTCGTGGGCTCGG-3′ | TTACTCGC | N952 | 5′-CAAGCAGAAGACGGCATACGAGAT GCTAACGAGTCTCGTGGGCTCGG-3′ | TCGTTAGC | N953 | 5′-CAAGCAGAAGACGGCATACGAGAT GCTCGGTAGTCTCGTGGGCTCGG-3′ | TACCGAGC | N954 | 5′-CAAGCAGAAGACGGCATACGAGAT GGAGAACAGTCTCGTGGGCTCGG-3′ | TGTTCTCC | N955 | 5′-CAAGCAGAAGACGGCATACGAGAT GGTGCGAAGTCTCGTGGGCTCGG-3′ | TTCGCACC | N956 | 5′-CAAGCAGAAGACGGCATACGAGAT GTACGCAAGTCTCGTGGGCTCGG-3′ | TTGCGTAC | N957 | 5′-CAAGCAGAAGACGGCATACGAGAT GTCGTAGAGTCTCGTGGGCTCGG-3′ | TCTACGAC | N958 | 5′-CAAGCAGAAGACGGCATACGAGAT GTCTGTCAGTCTCGTGGGCTCGG-3′ | TGACAGAC | N959 | 5′-CAAGCAGAAGACGGCATACGAGAT GTGTTCTAGTCTCGTGGGCTCGG-3′ | TAGAACAC | N960 | 5′-CAAGCAGAAGACGGCATACGAGAT TAGGATGAGTCTCGTGGGCTCGG-3′ | TCATCCTA |
| Index Primers for Illumina | Index | N961 | 5′-CAAGCAGAAGACGGCATACGAGAT TATCAGCAGTCTCGTGGGCTCGG-3′ | TGCTGATA | N962 | 5′-CAAGCAGAAGACGGCATACGAGAT TCCGTCTAGTCTCGTGGGCTCGG-3′ | TAGACGGA | N963 | 5′-CAAGCAGAAGACGGCATACGAGAT TCTTCACAGTCTCGTGGGCTCGG-3′ | TGTGAAGA | N964 | 5′-CAAGCAGAAGACGGCATACGAGAT TGAAGAGAGTCTCGTGGGCTCGG-3′ | TCTCTTCA | N965 | 5′-CAAGCAGAAGACGGCATACGAGAT TGGAACAAGTCTCGTGGGCTCGG-3′ | TTGTTCCA | N966 | 5′-CAAGCAGAAGACGGCATACGAGAT TGGCTTCAGTCTCGTGGGCTCGG-3′ | TGAAGCCA | N967 | 5′-CAAGCAGAAGACGGCATACGAGAT TGGTGGTAGTCTCGTGGGCTCGG-3′ | TACCACCA | N968 | 5′-CAAGCAGAAGACGGCATACGAGAT TTCACGCAGTCTCGTGGGCTCGG-3′ | TGCGTGAA | N969 | 5′-CAAGCAGAAGACGGCATACGAGAT AACTCACCGTCTCGTGGGCTCGG-3′ | GGTGAGTT | N970 | 5′-CAAGCAGAAGACGGCATACGAGAT AAGAGATCGTCTCGTGGGCTCGG-3′ | GATCTCTT | N971 | 5′-CAAGCAGAAGACGGCATACGAGAT AAGGACACGTCTCGTGGGCTCGG-3′ | GTGTCCTT | N972 | 5′-CAAGCAGAAGACGGCATACGAGAT AATCCGTCGTCTCGTGGGCTCGG-3′ | GACGGATT | N973 | 5′-CAAGCAGAAGACGGCATACGAGAT AATGTTGCGTCTCGTGGGCTCGG-3′ | GCAACATT | N974 | 5′-CAAGCAGAAGACGGCATACGAGAT ACACGACCGTCTCGTGGGCTCGG-3′ | GGTCGTGT | N975 | 5′-CAAGCAGAAGACGGCATACGAGAT ACAGATTCGTCTCGTGGGCTCGG-3′ | GAATCTGT | N976 | 5′-CAAGCAGAAGACGGCATACGAGAT AGATGTACGTCTCGTGGGCTCGG-3′ | GTACATCT | N977 | 5′-CAAGCAGAAGACGGCATACGAGAT AGCACCTCGTCTCGTGGGCTCGG-3′ | GAGGTGCT | N978 | 5′-CAAGCAGAAGACGGCATACGAGAT AGCCATGCGTCTCGTGGGCTCGG-3′ | GCATGGCT |
| Index Primers for Illumina | Index | N979 | 5′-CAAGCAGAAGACGGCATACGAGAT AGGCTAACGTCTCGTGGGCTCGG-3′ | GTTAGCCT | N980 | 5′-CAAGCAGAAGACGGCATACGAGAT ATAGCGACGTCTCGTGGGCTCGG-3′ | GTCGCTAT | N981 | 5′-CAAGCAGAAGACGGCATACGAGAT ATCATTCCGTCTCGTGGGCTCGG-3′ | GGAATGAT | N982 | 5′-CAAGCAGAAGACGGCATACGAGAT ATTGGCTCGTCTCGTGGGCTCGG-3′ | GAGCCAAT | N983 | 5′-CAAGCAGAAGACGGCATACGAGAT CAAGGAGCGTCTCGTGGGCTCGG-3′ | GCTCCTTG | N984 | 5′-CAAGCAGAAGACGGCATACGAGAT CACCTTACGTCTCGTGGGCTCGG-3′ | GTAAGGTG | N985 | 5′-CAAGCAGAAGACGGCATACGAGAT CCATCCTCGTCTCGTGGGCTCGG-3′ | GAGGATGG | N986 | 5′-CAAGCAGAAGACGGCATACGAGAT CCGACAACGTCTCGTGGGCTCGG-3′ | GTTGTCGG | N987 | 5′-CAAGCAGAAGACGGCATACGAGAT CCTAATCCGTCTCGTGGGCTCGG-3′ | GGATTAGG | N988 | 5′-CAAGCAGAAGACGGCATACGAGAT CCTCTATCGTCTCGTGGGCTCGG-3′ | GATAGAGG | N989 | 5′-CAAGCAGAAGACGGCATACGAGAT CGACACACGTCTCGTGGGCTCGG-3′ | GTGTGTCG | N990 | 5′-CAAGCAGAAGACGGCATACGAGAT CGGATTGCGTCTCGTGGGCTCGG-3′ | GCAATCCG | N991 | 5′-CAAGCAGAAGACGGCATACGAGAT CTAAGGTCGTCTCGTGGGCTCGG-3′ | GACCTTAG | N992 | 5′-CAAGCAGAAGACGGCATACGAGAT GAACAGGCGTCTCGTGGGCTCGG-3′ | GCCTGTTC | N993 | 5′-CAAGCAGAAGACGGCATACGAGAT GACAGTGCGTCTCGTGGGCTCGG-3′ | GCACTGTC | N994 | 5′-CAAGCAGAAGACGGCATACGAGAT GAGTTAGCGTCTCGTGGGCTCGG-3′ | GCTAACTC | N995 | 5′-CAAGCAGAAGACGGCATACGAGAT GATGAATCGTCTCGTGGGCTCGG-3′ | GATTCATC | N996 | 5′-CAAGCAGAAGACGGCATACGAGAT GCCAAGACGTCTCGTGGGCTCGG-3′ | GTCTTGGC |
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Products content
| N669983 | Component | 240 T | Storage | | N669983A | Index N501 Primers for Illumina | 240 μL | -20℃. Avoid freeze/thaw cycle. | | N669983B | Index N973-N996 Primers for Illumina | 24×10 μL | -20℃. Avoid freeze/thaw cycle. |
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Products Introduction
This kit is a companion kit to the transposase-based Rapid DNA Library Construction Kit for Illumina platform library construction. Each kit contains one N5 primer and 24 N7 primers, which can be used to prepare 24 different single-ended Index libraries. All reagents provided in the kits have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The libraries can be used for sequencing on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq.
Provide your own instruments, reagents and consumables
1. Magnetic frame: DynaMagTM-2 is recommended.
2. DNA purification and recovery kit: It is recommended to use DNA purification and recovery kit by magnetic bead method.
3. DNA building kit: It is recommended to use the Century transposase method second-generation sequencing rapid DNA building kit.
4. Anhydrous ethanol.
5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;
Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.
Pre-experiment Preparation and Important Notes
Please centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.
Procedure
For the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.
Index N501 Primer for Illumina
Index N901-N996 Primer for Illumina
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