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Northern Blot Stripping Solution (Mild)
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库存信息
| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| N743253-250ml |
250ml |
期货  |
|
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基本描述
| 别名 | Northern Blot 剥离液(温和) |
|---|---|
| 稳定性与储存 | 室温或4℃保存，一年有效。 |
| 英文名称 | Northern Blot Stripping Solution (Mild) |
| 储存温度 | 2-8°C储存 |
| 运输条件 | 冰袋运输 |
| 产品介绍 |
产品介绍： 阿拉丁生产的Northern Blot Stripping Solution (Mild)，即Northern印迹检测探针去除液(低强度)，也称Northern检测探针去除液或Northern探针去除液(低强度)，是一种用于Northern第一轮检测完成后需要再次进行内参RNA等的检测时用于高效去除结合在膜上的探针的溶液。本产品为DNase/RNase/Protease-free的无菌产品。本产品不仅可以去除RNA探针，也可以去除DNA探针；不仅可以去除生物素或地高辛标记探针，也可以去除同位素标记探针。通常短链探针的去除会相对更加容易，长链探针的去除会相对困难一点。Northern第一轮检测完成后，需要去除探针以再次进行内参RNA等的检测时，推荐使用阿拉丁生产的Northern Blot Stripping Solution (Mild) 、Northern Blot Stripping Solution (Moderate) 或Northern Blot Stripping Solution (Harsh) 这三种Northern探针去除液之一进行探针的去除。优先推荐尝试Northern Blot Stripping Solution (Moderate) ，如果去除效果欠佳可以尝试去除效果更强但操作不是很方便的Northern Blot Stripping Solution (Harsh) 。如有需要也可以使用去除效果更加温和的Northern Blot Stripping Solution (Mild) 。本产品可以和Biotin Northern Blot Kit 以及Biotin Northern Blot Kit (for Small RNA) 配合使用。本产品经DEPC (Diethyl pyrocarbonate，焦碳酸二乙酯)处理并高温高压灭菌处理，确保无DNase、RNase和Protease，即无DNA酶、RNA酶和蛋白酶。室温或4℃保存，一年有效。如果在低温时出现浑浊或沉淀，可以适当加热溶解，并充分混匀后使用。操作过程中，如果膜放置在容器中，需要确保膜的样品面向上。膜的取放推荐使用平头镊，推荐选购阿拉丁的 WB专用镊子(12cm, 翘方头)，每次取放膜不能触及有样品处。对于需要去除探针，再次进行Northern检测内参等RNA的情况，在第一轮印迹检测完毕之后，需要确保膜不会干燥。膜干燥后对于去除标记探针会带来很大的困难。由于环境中存在较多的RNase，使用过程中容易出现RNase污染而影响本产品的后续使用。操作过程中须严格注意避免RNase污染。宜戴口罩吸取本产品，并尽量减少本产品暴露于空气中的时间，取用后宜立即盖上盖子。本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。为了您的安全和健康，请穿实验服并戴一次性手套操作。使用说明： 1.对于需要去除探针，再次进行Northern检测内参等RNA的情况，在第一轮印迹检测完毕之后，需要确保膜不会干燥。膜干燥后对于去除标记探针会带来很大的困难。2.使用TE (Tris-EDTA缓冲液, DNase/RNase free)或SSC (2X)洗涤尼龙膜约5min，去除液体，并吸净残留液体。3.Stripping Solution在60℃预热。4.把膜转移至杂交管或杂交袋中，加入适量Stripping Solution，60℃孵育60min。孵育期间如果能适当摇动，通常能更好地去除结合的探针。5.取出尼龙膜，TE (Tris-EDTA缓冲液, DNase/RNase free)或SSC (2X)洗涤尼龙膜约5min，去除液体，并吸净残留液体。6.后续就可以再次进行Northern检测，或者加入适量TE (Tris-EDTA缓冲液, DNase/RNase free)或SSC (2X)后在4℃短期内保持，也可以干燥后室温长期保存。一旦干燥后，如果还有未充分去除的探针将很难去除。7.探针去除效果的检测，建议参考Biotin Northern Blot Kit 以及Biotin Northern Blot Kit (for Small RNA) 的说明书按照杂交完成后的所有步骤进行操作，最后观察是否还有原有探针的信号。8.去除探针后的Northern检测，推荐参考Biotin Northern Blot Kit 以及Biotin Northern Blot Kit (for Small RNA) 的说明书进行。Precautions： If turbidity or precipitation occurs in this product at low temperatures, the solution can be heated appropriately for dissolution and mixed well before use.During the operation, if the membrane is placed in a container, it is necessary to ensure that the sample side of the membrane is facing upwards. We recommend using ’s WB Special Tweezers (12cm, pointed flat head) for picking up and placing the membrane. Do not touch the sample area when handling the membrane.After the completion of the first blotting, do not allow the membrane dry out. Otherwise, it will be much more difficult to remove the labeled probes.Avoid RNase contamination during operation. Wear a disposable mask when using this product and minimize its exposure to air. After use, the lid should be immediately covered and tightened.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use： 1.Wash the nylon membrane with TE (Tris-EDTA buffer, DNase/RNase-free) or SSC (2X) for ~5 minutes, then discard all the liquid.2.Preheat the Northern Blot Stripping Solution at 60℃.3.Transfer the membrane to an appropriate container (e.g., hybridization tube or bag), add an appropriate amount of Stripping Solution, and incubate at 60℃ for 60 minutes. Gentle shaking is recommended to remove the bound probe more efficiently.4.Take the nylon membrane and wash it with TE (Tris-EDTA buffer, DNase/RNase-free) or SSC (2X) for ~5 minutes, then discard all the liquid.5.The stripped membrane can be hybridized again subsequently, or stored at 4℃ temporarily in an appropriate amount of TE (Tris-EDTA buffer, DNase/RNase-free) or SSC (2X), or stored at room temperature for a longer period of time after drying. Once dried, it will be difficult to remove any remaining probes on the membrane.6.To test the effectiveness of probe removal, follow all the steps after hybridization according to the instructions of Quick Hybridization Solution500mlR0251-500mlLow Stringency Wash Solution (DNase/RNase free)500mlR0253-500mlHigh Stringency Wash Solution (DNase/RNase free)500mlR0262-250mlNorthern Blot Stripping Solution (Mild)250mlR0266-250mlNorthern Blot Stripping Solution (Moderate)250mlR0268-250mlNorthern Blot Stripping Solution (Harsh)250mlA0303HRP-labeled Streptavidin0.2mlP0018F Ultrapure Water (DNase/RNase-Free, Sterile)100/500mlR0021DEPC-water (DNase、RNase free)100mlR0022DEPC-water (DNase、RNase free)500mlST004LAgarose50gST004B40% Acr-Bis (19:1, Nuclease free)100mlR0123RNase and DNase Away250mlR0125RNase, DNase and DNA Away250mlR0127RNase, DNase, DNA and RNA Away250ml |
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