库恩对哈姆氏 F-12 的改造是为培养通过病毒融合产生的杂交细胞而开发的。修饰包括使氨基酸和丙酮酸加倍,并包括抗坏血酸。盐浓度也发生了改变。配方中含有 0.863 mg/L 硫酸锌,这可能使其不适合培养小鼠 L -细胞。
Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.
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用于细胞培养, powder, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, Coon′s Modification