Human Factor Alpha XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. Human Factor XII and prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates prekallikrein to kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. ERL offers Factor α-XIIa which is activated by the autoactivation process with Dextran Sulfate and re-purified to remove the activator. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.
Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
Extinction Coefficient (1%) = 14.1
Molecular Weight = 80,000 daltons
The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
规格
产品名称
凝血因子Alpha XIIa
别名
人源凝血因子Alpha XIIa
英文别名
Human Factor Alpha XIIa | Human Factor Alpha-XIIa | Human Factor αXIIa | Activated Hageman Factor | Coagulation Factor α-XIIa | Coagulation factor | Coagulation factor XII | F12 | HAF | HAE3 | HAEX
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Post-translational: Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
生物活性
见COA
种属
人(Human)
来源
天然
预测分子量
80 kDa
酶学委员会编号
EC 3.4.21.38
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 4 mM Sodium acetate-HCl, 0.15 M NaCl, pH 5.3.
浓度
来源于人血浆; 冻干前浓度：见COA
复溶
We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.
危险品化学品经营许可证（带存储）