计算溶液所需的质量、体积或浓度。
原果胶 (SP)提取试剂
有货
为什么选择此级别
生物活性 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
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文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
天然果胶类物质以原果胶、果胶 (Pectin)、果胶酸的形态广泛存在于植物的果实、根、茎、叶中，是细胞壁的一种组成成分，它们伴随纤维素而存在，构成相邻细胞中间层粘结物，使植物组织细胞紧紧黏结在一起。原果胶是不溶于水的物质，但可在酸、碱、盐等化学试剂及酶的作用下，加水分解转变成水溶性果胶。果胶 (Pectin)又称多聚半乳糖醛酸，是由D-半乳糖醛酸以α-1,4糖苷键连接形成的直链状聚合物，本质上是一种线形的多糖聚合物，含有数百至约1000个脱水半乳糖醛酸残基，其相应的平均相对分子质量为50000-150000。原果胶是一种非水溶性的物质，在未成熟的果实或植物组织中果胶物质大多与纤维素结合以原果胶的形式存在，原果胶能使果实或其他植物组织坚硬、变脆。
原果胶 (SP)提取试剂主要用于提取植物组织或果实中可溶性果胶和原果胶。该试剂仅用于科研领域，不宜用于临床诊断或其他用途。
产品组成：
|
自备材料：
1、蒸馏水
2、实验材料：桃子、李子、苹果、杏等果实或其他植物组织
3、天平、剪刀、研钵或匀浆器、离心机、水浴锅、试管或离心管
操作步骤 (仅供参考)：
1、取果实或其他植物组织，洗净擦干，称取剪碎的新鲜样品0.2 g，置于研钵或匀浆器。
2、加入1-2 ml可溶性果胶提取试剂，充分研磨或匀浆后转入10 ml试管中。用可溶性果胶提取试剂冲洗研钵或匀浆器并转移至试管中，补加可溶性果胶提取试剂至10 ml。
3、沸水浴30 min，在煮沸过程中及时补加可溶性果胶提取试剂至10 ml，取出冷却至室温，8000 r/min离心15 min，弃上清。重复该步骤2次，以去除样品中的糖分及其他物质。
4、取含有沉淀的试管，加入4 ml蒸馏水，50℃水浴30 min以溶解果胶。取出冷却至室温，8000 r/min离心15 min，将上清液转移至新试管中。用少量蒸馏水洗涤沉淀，8000 r/min再次离心15 min，一并将上清液转移至上述新试管中，加蒸馏水定容至10 ml，即为可溶性果胶提取液。
5、向上述经蒸馏水洗涤后的沉淀物中加入3 ml原果胶提取试剂，沸水浴1 h，取出冷却至室温，8000 r/min离心15 min，加蒸馏水定容至10 ml，即为原果胶提取液。
注意事项：
1、取样量、试剂用量应根据果胶含量适当调整。
2、可溶性糖对测定结果有较大影响，应彻底去除样品中的可溶性糖。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Natural pectin substances exist extensively in the fruits, roots, stems and leaves of plants in the forms of protopectin, pectin and pectic acid, and serve as a component of plant cell walls. They coexist with cellulose and form the cementing substance in the middle lamella between adjacent cells, binding plant tissue cells tightly together. Protopectin is water-insoluble, but it can be hydrolyzed into water-soluble pectin under the action of chemical reagents such as acids, alkalis and salts, as well as enzymes with the addition of water. Pectin, also known as polygalacturonic acid, is a linear polymer formed by the linkage of D-galacturonic acid via α-1,4 glycosidic bonds and is essentially a linear polysaccharide polymer. It contains hundreds to about 1000 anhydrogalacturonic acid residues, with a corresponding average relative molecular mass of 50,000-150,000. Protopectin is a water-insoluble substance; in immature fruits or plant tissues, most pectin substances combine with cellulose and exist in the form of protopectin, which imparts hardness and crispness to fruits and other plant tissues.
Protopectin (SP) Extraction Reagent is mainly used for the extraction of soluble pectin and protopectin from plant tissues or fruits. This reagent is for research use only (RUO) and not intended for clinical diagnosis or other applications.
Product Composition
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Materials Provided by User
1. Distilled water
2. Experimental materials: fruits such as peaches, plums, apples, apricots, or other plant tissues
3. Balance, scissors, mortar or homogenizer, centrifuge, water bath, test tubes or centrifuge tubes
Operating Procedures (For Reference Only)
1. Take the fruit or other plant tissues, wash and dry them thoroughly. Weigh 0.2 g of the cut fresh sample and place it in a mortar or homogenizer.
2. Add 1-2 mL of Soluble Pectin Extraction Reagent, grind or homogenize the sample fully, and then transfer the mixture to a 10 mL test tube. Rinse the mortar or homogenizer with Soluble Pectin Extraction Reagent and transfer the rinse solution to the same test tube, then make up the volume to 10 mL with the same reagent.
3. Incubate the test tube in a boiling water bath for 30 minutes; replenish Soluble Pectin Extraction Reagent in a timely manner to maintain the volume at 10 mL during boiling. Take out the test tube and cool it to room temperature, then centrifuge at 8000 r/min for 15 minutes and discard the supernatant. Repeat this step twice to completely remove sugars and other impurities from the sample.
4. Take the test tube containing the precipitate, add 4 mL of distilled water, and incubate in a 50 ℃ water bath for 30 minutes to dissolve the pectin. Take out the test tube and cool it to room temperature, centrifuge at 8000 r/min for 15 minutes, and transfer the supernatant to a new test tube. Wash the precipitate with a small amount of distilled water, centrifuge again at 8000 r/min for 15 minutes, transfer the resulting supernatant to the aforementioned new test tube, and make up the volume to 10 mL with distilled water. This is the soluble pectin extract.
5. Add 3 mL of Protopectin Extraction Reagent to the precipitate washed with distilled water in the previous step, incubate in a boiling water bath for 1 hour, take out the test tube and cool it to room temperature, then centrifuge at 8000 r/min for 15 minutes. Make up the volume to 10 mL with distilled water; this is the protopectin extract.
Precautions
1. The sample amount and reagent volume should be adjusted appropriately according to the pectin content in the sample.
2. Soluble sugars have a significant impact on the determination results; thus, soluble sugars in the sample must be completely removed.
3. Please use the reagent as soon as possible after opening the package to avoid affecting the results of subsequent experiments.
4. For your safety and health, wear a lab coat and disposable gloves during the operation.
规格
别名
原果胶提取试剂 | SP提取试剂
英文别名
Protopectin Extraction Reagent | SP Extraction Reagent
规格或纯度
生物活性,Suitable for plant cell and tissue extracts
稳定性与储存
各组分在相应的储存条件下保质期2年。
英文名称
Protopectin (SP) Extraction Reagent
储存条件
室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434099 |
分析证书 | O1509542 |
技术文档和文章
溶液计算器
常见问题
本产品应如何储存？
请于室温条件下保存。
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